A site-directed mutagenesis method particularly useful for creating otherwise difficult-to-make mutants and alanine scanning

Analytical Biochemistry

Volumn 420, Issue 2, 2012, Pages 163-170

  Wan, Haisu ^a   Li, Yongwen ^a   Fan, Yu ^a   Meng, Fanrong ^a   Chen, Chen ^a   Zhou, Qinghua ^a  

^a TIANJIN MEDICAL UNIVERSITY   (China)

Author keywords

Difficult to make mutants; Gene structure; Mutagenic fragments; Mutagenic primers; Site directed mutagenesis

Indexed keywords

AMINO ACIDS; ENZYMES; GENES; MOLECULAR BIOLOGY; MUTAGENESIS;

DIFFICULT-TO-MAKE MUTANTS; GENE STRUCTURE; MUTAGENIC FRAGMENTS; MUTAGENIC PRIMERS; SITE DIRECTED MUTAGENESIS;

POLYMERASE CHAIN REACTION;

ALANINE; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

AMINO ACID ANALYSIS; ARTICLE; MUTAGENICITY; MUTANT; MUTATION RATE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

EID: 81155128586     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.09.019     Document Type: Article

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