An efficient approach for site-directed mutagenesis using central overlapping primers

Analytical Biochemistry

Volumn 418, Issue 2, 2011, Pages 304-306

  Wang, Huiping ^a   Zhou, Nan ^a   Ding, Feng ^a   Li, Zhaofang ^a   Chen, Ruichuan ^a   Han, Aidong ^a   Liu, Runzhong ^a  

^a XIAMEN UNIVERSITY   (China)

Author keywords

Central overlapping primers (COP); QuikChange; Site directed mutagenesis

Indexed keywords

ESCHERICHIA COLI; NUCLEIC ACIDS;

CENTRAL OVERLAPPING PRIMERS (COP); ESCHERICHIA COLI CELLS; MUTATION RATES; NOVEL STRATEGIES; PRIMER DESIGN; QUIKCHANGE; SITE DIRECTED MUTAGENESIS; STANDARD PROTOCOLS;

MUTAGENESIS;

CYCLIN DEPENDENT KINASE 9; PRIMER DNA; TRANSCRIPTION ELONGATION FACTOR;

ANALYTIC METHOD; ARTICLE; CENTRAL OVERLAPPING PRIMER; DNA SEQUENCE; ESCHERICHIA COLI; GENE; GENE AMPLIFICATION; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 81155162596     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.07.008     Document Type: Article

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