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Volumn 38, Issue 6, 2005, Pages 864-868

Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DNA; DNA POLYMERASE; MUTANT PROTEIN; PHENFORMIN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; DNA DIRECTED DNA POLYMERASE; PFU DNA POLYMERASE; PRIMER DNA;

EID: 27744439858     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05386BM03     Document Type: Article
Times cited : (28)

References (7)
  • 3
    • 16544395270 scopus 로고    scopus 로고
    • Site-directed, ligase-independent mutagenesis (SLIM): A single-tube methodology approaching 100% efficiency in 4 h
    • Chiu, J., P.E. March, R. Lee, and D. Tillett. 2004. Site-directed, ligase-independent mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. Nucleic Acids Res. 32:e 174.
    • (2004) Nucleic Acids Res. , vol.32
    • Chiu, J.1    March, P.E.2    Lee, R.3    Tillett, D.4
  • 4
    • 0025325983 scopus 로고
    • The megaprimer method of site-directed mutagenesis
    • Sarkar, G. and S.S. Sommer. 1990. The "megaprimer" method of site-directed mutagenesis. BioTechniques 8:404-407.
    • (1990) BioTechniques , vol.8 , pp. 404-407
    • Sarkar, G.1    Sommer, S.S.2
  • 5
    • 0346992128 scopus 로고    scopus 로고
    • A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product
    • Nagy, Z.B., F. Felfoldi, L. Tamas, and L.G. Puskas. 2004. A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product. Anal. Biochem. 524:301-303.
    • (2004) Anal. Biochem. , vol.524 , pp. 301-303
    • Nagy, Z.B.1    Felfoldi, F.2    Tamas, L.3    Puskas, L.G.4
  • 6
    • 0026786146 scopus 로고
    • Enzymatic inverse PCR: A restriction site independent, single fragment method for high-efficiency, site-directed mutagenesis
    • Stemmer, W.P. and S.K. Morris. 1992. Enzymatic inverse PCR: a restriction site independent, single fragment method for high-efficiency, site-directed mutagenesis. BioTechniques 13:214-220.
    • (1992) BioTechniques , vol.13 , pp. 214-220
    • Stemmer, W.P.1    Morris, S.K.2
  • 7
    • 1542375914 scopus 로고    scopus 로고
    • Versatile PCR-mediated insertion or deletion mutagenesis
    • Lee, J., H.J. Lee, M.K. Shin, and W.S. Ryu. 2004. Versatile PCR-mediated insertion or deletion mutagenesis. BioTechniques 36:398-400.
    • (2004) BioTechniques , vol.36 , pp. 398-400
    • Lee, J.1    Lee, H.J.2    Shin, M.K.3    Ryu, W.S.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.