A one-step PCR-based method for rapid and efficient site-directed fragment deletion, insertion, and substitution mutagenesis

Journal of Virological Methods

Volumn 149, Issue 1, 2008, Pages 85-90

  Qi, Dong ^a   Scholthof, Karen Beth G ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Partially complementary primer; Protein genetic engineering; Site directed fragment mutagenesis

Indexed keywords

CAPSID PROTEIN;

ARTICLE; CONTROLLED STUDY; GENE DELETION; GENE INSERTION; MOSAIC VIRUS; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SUBSTITUTION REACTION; VIRUS CELL INTERACTION;

CAPSID PROTEINS; DNA PRIMERS; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; PLANTS; POLYMERASE CHAIN REACTION; SATELLITE VIRUSES; SEQUENCE DELETION; TOMBUSVIRIDAE;

MIRIDAE; PANICUM MOSAIC SATELLITE VIRUS;

EID: 40849089190     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.01.002     Document Type: Article

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