Efficient production of adenovirus vector lacking genes of virus-associated RNAs that disturb cellular RNAi machinery

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Maekawa, Aya ^a   Pei, Zheng ^a   Suzuki, Mariko ^a   Fukuda, Hiromitsu ^a,b   Ono, Yohei ^a   Kondo, Saki ^a   Saito, Izumu ^a   Kanegae, Yumi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b Japan Animal Referral Medical Center   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

MICRORNA; VIRUS RNA;

ADENOVIRUS; ARTICLE; CELL LINE; CELL STRAIN HEK293; GENE VECTOR; GENETICS; HELA CELL; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RNA INTERFERENCE;

ADENOVIRIDAE; BASE SEQUENCE; CELL LINE; GENETIC VECTORS; HEK293 CELLS; HELA CELLS; HUMANS; MICRORNAS; MOLECULAR SEQUENCE DATA; RNA INTERFERENCE; RNA, VIRAL;

MLCS; MLOWN;

EID: 84873156800     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep01136     Document Type: Article

References (34)

1. Miyake, S. et al. Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA 93, 1320-1324 (1996).
2. Anton, M. & Graham, F. L. Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: amolecular switch for control of gene expression. J. Virol. 69, 4600-4606 (1995).
3. Lu, S. & Cullen, B. R. Adenovirus VA1 noncoding RNA can inhibit small interfering RNA and MicroRNA biogenesis. J. Virol. 78, 12868-12876 (2004). (Pubitemid 39507795)
4. Carnero, E., Sutherland, J. D. & Fortes, P. Adenovirus and miRNAs. Biochim. Biophys. Acta 1809, 660-667 (2011).
5. Aparicio, O. et al. Adenovirus VA RNA-derived miRNAs target cellular genes involved in cell growth, gene expression and DNA repair. Nucleic Acids Res 38, 750-763 (2010).
6. Bhat, R. A., Domer, P. H. & Thimmappaya, B. Structural requirements of adenovirus VAI RNA for its translation enhancement function. Mol. Cell Biol. 5, 187-196 (1985). (Pubitemid 15164285)
7. Machitani, M. et al. Development of an adenovirus vector lacking the expression of virus-associated RNAs. J. Control Release 154, 285-289 (2011).
8. Takata, Y., Kondo, S., Goda, N., Kanegae, Y. & Saito, I. Comparison of efficiency between FLPe and Cre for recombinase-mediated cassette exchange in vitro and in adenovirus vector production. Genes Cells 16, 765-777 (2011).
9. Buchholz, F., Angrand, P. O. & Stewart, A. F. Improved properties of FLP recombinase evolved by cycling mutagenesis. Nat. Biotechnol. 16, 657-662 (1998). (Pubitemid 28334324)
10. Kanegae, Y. et al. High-level expression by tissue/cancer-specific promoter with strict specificity using a single-adenoviral vector. Nucleic Acids Res. 39, e7 (2011).
11. Kondo, S., Takata, Y.,Nakano, M., Saito, I. & Kanegae, Y. Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol. 390, 221-230 (2009).
12. Pei, Z., Kondo, S., Kanegae, Y. & Saito, I. Copy number of adenoviral vector genome transduced into target cells can be measured using quantitative PCR: application to vector titration. Biochem. Biophys. Res. Commun. 417, 945-950 (2012).
13. Ng, P., Evelegh, C., Cummings, D. & Graham, F. L. Cre levels limit packaging signal excision efficiency in the Cre/loxP helper-dependent adenoviral vector system. J. Virol. 76, 4181-4189 (2002). (Pubitemid 34309553)
14. Ng, P., Parks, R. J. & Graham, F. L. Preparation of helper-dependent adenoviral vectors. Methods Mol. Med. 69, 371-388 (2002).
15. Loonstra, A. et al. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc. Natl. Acad. Sci. USA 98, 9209-9214 (2001). (Pubitemid 32743895)
16. Pfeifer, A., Brandon, E. P., Kootstra, N., Gage, F. H. & Verma, I. M. Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Proc. Natl. Acad. Sci. USA 98, 11450-11455 (2001). (Pubitemid 32928749)
17. Silver, D. P. & Livingston, D. M. Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity. Mol. Cell 8, 233-243 (2001). (Pubitemid 32772921)
18. Baba, Y., Nakano, M., Yamada, Y., Saito, I. & Kanegae, Y. Practical range of effective dose for Cre recombinase-expressing recombinant adenovirus without cell toxicity in mammalian cells. Microbiol. Immunol. 49, 559-570 (2005). (Pubitemid 40943836)
19. Saito, I., Oya, Y., Yamamoto, K., Yuasa, T. & Shimojo, H. Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome. J. Virol. 54, 711-719 (1985). (Pubitemid 15014703)
20. Nakai, M. et al. Expression of pIX gene induced by transgene promoter: possible cause of host immune response in first-generation adenoviral vectors. Hum. Gene Ther. 18, 925-936 (2007). (Pubitemid 47587319)
21. Lee, G. & Saito, I. Role of nucleotide sequences of loxP spacer region in Cremediated recombination. Gene 216, 55-65 (1998). (Pubitemid 28389102)
22. Schlake, T. & Bode, J. Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry 33, 12746-12751 (1994). (Pubitemid 24352862)
23. Nakano, M., Ishimura, M., Chiba, J., Kanegae, Y. & Saito, I. DNA substrates influence the recombination efficiency mediated by FLP recombinase expressed in mammalian cells. Microbiol. Immunol. 45, 657-665 (2001). (Pubitemid 32895925)
24. Graham, F. L., Smiley, J., Russell, W. C.&Nairn, R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36, 59-74 (1977).
25. Precious, B.&Russell, W. C. Growth, purification and titration of adenoviruses. In Virology: a practical approach. (ed. Mahy, B. W. J.) 128-152 (IRL press, Oxford; 1991).
26. Kanegae, Y., Makimura, M. & Saito, I. A simple and efficient method for purification of infectious recombinant adenovirus. Jpn J. Med. Sci. Biol. 47, 157-166 (1994). (Pubitemid 24330700)
27. Nakano, M. et al. Efficient gene activation in cultured mammalian cells mediated by FLP recombinase-expressing recombinant adenovirus. Nucleic Acids Res. 29, E40 (2001).
28. Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y. & Saito, I. Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol. 50, 643-654 (2006). (Pubitemid 44285292)
29. Kim, D. W.,Uetsuki, T., Kaziro, Y., Yamaguchi, N. & Sugano, S. Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system. Gene 91, 217-223 (1990). (Pubitemid 20269505)
30. Chiyo, T. et al. Conditional gene expression in hepatitis C virus transgenic mice without induction of severe liver injury using a non-inflammatory Cre-expressing adenovirus. Virus Res. 160, 89-97 (2011).
31. Kanegae, Y. et al. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res. 23, 3816-3821 (1995).
32. Takebe, Y. et al. SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell Biol. 8, 466-472 (1988). (Pubitemid 18015592)
33. Saito, I., Groves, R., Giulotto, E., Rolfe, M. & Stark, G. R. Evolution and stability of chromosomal DNA coamplified with the CAD gene. Mol. Cell Biol. 9, 2445-2452 (1989).
34. Nakano, M. et al. Production of viral vectors using recombinase-mediated cassette exchange. Nucleic Acids Res. 33, e76 (2005).