Activities of Various FLP Recombinases Expressed by Adenovirus Vectors in Mammalian Cells

Journal of Molecular Biology

Volumn 390, Issue 2, 2009, Pages 221-230

  Kondo, Saki ^a   Takata, Yuki ^a,c   Nakano, Masakazu ^a,b   Saito, Izumu ^a   Kanegae, Yumi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b KYOTO PREFECTURAL UNIVERSITY OF MEDICINE   (Japan)

^c Kokando Co Ltd   (Japan)

Author keywords

adenovirus vector; FLP; FLPe; mammalian cell; recombinase

Indexed keywords

ADENOVIRUS VECTOR; CRE RECOMBINASE; RECOMBINASE;

ARTICLE; CELL LINE; CODON USAGE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; GENE CONSTRUCT; GENE DELETION; GENETIC TRANSDUCTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MAMMAL CELL; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; STEADY STATE; TEMPERATURE; THERMOSTABILITY; TRANSGENIC MOUSE; VIRUS GENOME; WILD TYPE;

ADENOVIRIDAE; ANIMALIA; MAMMALIA; MUS MUSCULUS;

EID: 67349130781     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2009.04.057     Document Type: Article

References (43)

1. Luo H., Lyznik L.A., Gidoni D., and Hodges T.K. FLP-mediated recombination for use in hybrid plant production. Plant J. 23 (2000) 423-430
2. Riou-Khamlichi C., Huntley R., Jacqmard A., and Murray J.A. Cytokinin activation of Arabidopsis cell division through a D-type cyclin. Science 283 (1999) 1541-1544
3. Golic K.G., and Lindquist S. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell 59 (1989) 499-509
4. Rong Y.S., and Golic K.G. Gene targeting by homologous recombination in Drosophila. Science 288 (2000) 2013-2018
5. Aladjem M.I., Brody L.L., O'Gorman S., and Wahl G.M. Positive selection of FLP-mediated unequal sister chromatid exchange products in mammalian cells. Mol. Cell. Biol. 17 (1997) 857-861
6. Aladjem M.I., Rodewald L.W., Kolman J.L., and Wahl G.M. Genetic dissection of a mammalian replicator in the human beta-globin locus. Science 281 (1998) 1005-1009
7. O'Gorman S., Fox D.T., and Wahl G.M. Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science 251 (1991) 1351-1355
8. Vooijs M., van der Valk M., te Riele H., and Berns A. Flp-mediated tissue-specific inactivation of the retinoblastoma tumor suppressor gene in the mouse. Oncogene 17 (1998) 1-12
9. Dymecki S.M. FLP recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice. Proc. Natl Acad. Sci. USA 93 (1996) 6191-6196
10. Dymecki S.M., and Tomasiewicz H. Using Flp-recombinase to characterize expansion of Wnt1-expressing neural progenitors in the mouse. Dev. Biol. 201 (1998) 57-65
11. Barinaga M. Knockout mice: round two. Science 265 (1994) 26-28
12. Fukushige S., and Sauer B. Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proc. Natl Acad. Sci. USA 89 (1992) 7905-7909
13. Rossant J., and Nagy A. Genome engineering: the new mouse genetics. Nat. Med. 1 (1995) 592-594
14. Sauer B., and Henderson N. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Proc. Natl Acad. Sci. USA 85 (1988) 5166-5170
15. Loonstra A., Vooijs M., Beverloo H.B., Allak B.A., van Drunen E., Kanaar R., et al. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc. Natl Acad. Sci. USA 98 (2001) 9209-9214
16. Pfeifer A., Brandon E.P., Kootstra N., Gage F.H., and Verma I.M. Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Proc. Natl Acad. Sci. USA 98 (2001) 11450-11455
17. Silver D.P., and Livingston D.M. Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity. Mol. Cell 8 (2001) 233-243
18. Baba Y., Nakano M., Yamada Y., Saito I., and Kanegae Y. Practical range of effective dose for Cre recombinase-expressing recombinant adenovirus without cell toxicity in mammalian cells. Microbiol. Immunol. 49 (2005) 559-570
19. Buchholz F., Ringrose L., Angrand P.O., Rossi F., and Stewart A.F. Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination. Nucleic Acids Res. 24 (1996) 4256-4262
20. Buchholz F., Angrand P.O., and Stewart A.F. Improved properties of FLP recombinase evolved by cycling mutagenesis. Nat. Biotechnol. 16 (1998) 657-662
21. Beard C., Hochedlinger K., Plath K., Wutz A., and Jaenisch R. Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Genesis 44 (2006) 23-28
22. Lauth M., Spreafico F., Dethleffsen K., and Meyer M. Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases. Nucleic Acids Res. 30 (2002) e115
23. Schaft J., Ashery-Padan R., van der Hoeven F., Gruss P., and Stewart A.F. Efficient FLP recombination in mouse ES cells and oocytes. Genesis 31 (2001) 6-10
24. Farley F.W., Soriano P., Steffen L.S., and Dymecki S.M. Widespread recombinase expression using FLPeR (flipper) mice. Genesis 28 (2000) 106-110
25. Kanki H., Suzuki H., and Itohara S. High-efficiency CAG-FLPe deleter mice in C57BL/6J background. Exp. Anim. 55 (2006) 137-141
26. Rodriguez C.I., Buchholz F., Galloway J., Sequerra R., Kasper J., Ayala R., et al. High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP. Nat. Genet. 25 (2000) 139-140
27. Andreas S., Schwenk F., Kuter-Luks B., Faust N., and Kuhn R. Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells. Nucleic Acids Res. 30 (2002) 2299-2306
28. Raymond C.S., and Soriano P. High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. PLoS ONE 2 (2007) e162
29. Anton M., and Graham F.L. Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression. J. Virol. 69 (1995) 4600-4606
30. Kanegae Y., Lee G., Sato Y., Tanaka M., Nakai M., Sakaki T., et al. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res. 23 (1995) 3816-3821
31. Kondo S., Okuda A., Sato H., Tachikawa N., Terashima M., Kanegae Y., and Saito I. Simultaneous on/off regulation of transgenes located on a mammalian chromosome with Cre-expressing adenovirus and a mutant loxP. Nucleic Acids Res. 31 (2003) e76
32. Shibata H., Toyama K., Shioya H., Ito M., Hirota M., Hasegawa S., et al. Rapid colorectal adenoma formation initiated by conditional targeting of the Apc gene. Science 278 (1997) 120-123
33. Shintani Y., Yotsuyanagi H., Moriya K., Fujie H., Tsutsumi T., Kanegae Y., et al. Induction of apoptosis after switch-on of the hepatitis B virus X gene mediated by the Cre/loxP recombination system. J. Gen. Virol. 80 Pt12 (1999) 3257-3265
34. Nakano M., Odaka K., Ishimura M., Kondo S., Tachikawa N., Chiba J., et al. Efficient gene activation in cultured mammalian cells mediated by FLP recombinase-expressing recombinant adenovirus. Nucleic Acids Res. 29 (2001) e40
35. Kondo S., Takahashi Y., Shiozawa S., Ichise H., Yoshida N., Kanegae Y., and Saito I. Efficient sequential gene regulation via FLP-and Cre-recombinase using adenovirus vector in mammalian cells including mouse ES cells. Microbiol. Immunol. 50 (2006) 831-843
36. Takashima Y., Era T., Nakano K., Kondo S., Kasuga M., Smith A.G., and Nishikawa S. Neuroepithelial cells supply an initial transient wave of MSC differentiation. Cell 129 (2007) 1377-1388
37. Kanegae Y., Makimura M., and Saito I. A simple and efficient method for purification of infectious recombinant adenovirus. Jpn. J. Med. Sci. Biol. 47 (1994) 157-166
38. Graham F.L., Smiley J., Russell W.C., and Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36 (1977) 59-74
39. Fukuda H., Terashima M., Koshikawa M., Kanegae Y., and Saito I. Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol. 50 (2006) 643-654
40. Kozak M. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 9 (1981) 5233-5252
41. Niwa H., Yamamura K., and Miyazaki J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108 (1991) 193-199
42. Wada K., Aota S., Tsuchiya R., Ishibashi F., Gojyobori T., and Ikemura T. Codon usage tabulated from the GenBank genetic sequence data. Nucleic Acids Res. 25 (1990) 2367-2411
43. Chen Y., Narendra U., Iype L.E., Cox M.M., and Rice P.A. Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping. Mol. Cell 6 (2000) 885-897