DNA substrates influence the recombination efficiency mediated by FLP recombinase expressed in mammalian cells

Microbiology and Immunology

Volumn 45, Issue 9, 2001, Pages 657-665

  Nakano, M ^a   Ishimura, M ^a   Chiba, J ^a   Kanegae, Y ^a   Saito, I ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cre loxP system; FLP FRT system; Mutant FRT; Site specific recombinase

Indexed keywords

DNA; RECOMBINASE;

ADENOVIRUS; ANIMAL CELL; ARTICLE; BACTERIOPHAGE; DNA RECOMBINATION; GENE CONTROL; GENETIC ANALYSIS; MAMMAL; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; VIRUS RECOMBINANT;

ANIMALS; CELLS, CULTURED; DNA; DNA NUCLEOTIDYLTRANSFERASES; GENE TRANSFER TECHNIQUES; MAMMALS; RECOMBINATION, GENETIC;

EID: 0034837588     PISSN: 03855600     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2001.tb01299.x     Document Type: Article

References (23)

1. The FLP recombinase of the 2 micron circle DNA of yeast: Interaction with its target sequences
2. Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: A molecular switch for control of gene expression
3. Crystal structure of a Flp recombinase-holliday junction complex: Assembly of an active oligomer by helix swapping
4. Structure and mechanism in site-specific recombination
5. Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse
6. Asymmetric DNA bending in the Cre-loxP site-specific recombination synapse
7. The cis-trans paradox of integrase
8. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase
9. Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase
10. Site-specific recombinases: Tools for genome engineering
11. Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination
12. Identification of the crossover site during FLP-mediated recombination in the Saccharomyces cerevisiae plasmid 2 microns circle
13. Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome
14. Efficient gene activation in cultured mammalian cells mediated by FLP recombinase-expressing recombinant adenovirus
15. Development of a flp/frt system for generating helper-dependent adenoviral vectors
16. Efficient selection for high-expression transfectants with a novel eukaryotic vector
17. Site-specific recombination: Developments and applications
18. Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci
19. Double-reciprocal crossover mediated by FLP-recombinase: A concept and an assay
20. The FLP recombinase of the yeast 2-micron plasmid: Characterization of its recombination site
21. Directionality in FLP protein-promoted site-specific recombination is mediated by DNA-DNA pairing
22. Efficient FLPe recombinase enables scalable production of helper-dependent adenoviral vectors with negligible helper-virus contamination
23. The functional significance of DNA sequence structure in a site-specific genetic recombination reaction