Fluorescent ligands to investigate GPCR binding properties and oligomerization

Biochemical Society Transactions

Volumn 41, Issue 1, 2013, Pages 148-153

  Cottet, Martin ^a,b,c   Faklaris, Orestis ^a,b,c   Falco, Amadine ^a,b,c   Trinquet, Eric ^d   Pin, Jean Philippe ^a,b,c   Mouillac, Bernard ^a,b,c   Durroux, Thierry ^a,b,c  

^a CNRS   (France)

^b INSTITUT DE GÉNOMIQUE FONCTIONNELLE   (France)

^c UNIV MONTPELLIER   (France)

^d Cisbio Bioassays ^*  (France)

Author keywords

Europium; Fluorescent ligand; G protein coupled receptor; Lanthanide; Terbium; Timeresolved FRET

Indexed keywords

ALEXA FLUOR 647; DY 647; EUROPIUM; FLUORESCEIN; G PROTEIN COUPLED RECEPTOR; LANTHANIDE; OXYTOCIN DERIVATIVE; OXYTOCIN RECEPTOR; TERBIUM; TERBIUM CRYPTATE; UNCLASSIFIED DRUG;

ANIMAL TISSUE; BINDING ASSAY; CHEMICAL LABELING; CONFERENCE PAPER; DRUG SCREENING; FEMALE; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; LIGAND BINDING; LUMINESCENCE; MAMMARY GLAND; MOLECULAR INTERACTION; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; RECEPTOR AFFINITY; RECEPTOR BINDING; SIGNAL NOISE RATIO; WILD TYPE;

BIOPOLYMERS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; LIGANDS; PROTEIN BINDING; RECEPTORS, G-PROTEIN-COUPLED;

EID: 84873103005     PISSN: 03005127     EISSN: 14708752     Source Type: Journal    
DOI: 10.1042/BST20120237     Document Type: Conference Paper

References (40)

1. Mouillac, B., Manning, M. and Durroux, T. (2008) Fluorescent agonists and antagonists for vasopressin/oxytocin G protein-coupled receptors: usefulness in ligand screening assays and receptor studies. Mini-Rev. Med. Chem. 8, 996-1005
2. Durroux, T., Peter, M., Turcatti, G., Chollet, A., Balestre, M. N., Barberis, C. and Seyer, R. (1999) Fluorescent pseudo-peptide linear vasopressin antagonists: design, synthesis, and applications. J. Med. Chem. 42, 1312-1319 (Pubitemid 29181153)
3. 1a receptors. J. Med. Chem. 45, 2579-2588 (Pubitemid 34595228)
4. Kirk, K. L., Buku, A. and Eggena, P. (1987) Cell specificity of vasopressin binding in renal collecting duct: computer-enhanced imaging of a fluorescent hormone analog. Proc. Natl. Acad. Sci. U. S. A. 84, 6000-6004
5. Lutz, W. H., Londowski, J. M. and Kumar, R. (1990) The synthesis and biological activity of four novel fluorescent vasopressin analogs. J. Biol. Chem. 265, 4657-4663
6. Förster, T. (1948) Zwischenmolekulare Energiewanderung und Fluoreszenz. Annalen des Physik 2, 55-75
7. Ayoub, M. A. and Pfleger, K. D. (2010) Recent advances in bioluminescence resonance energy transfer technologies to study GPCR heteromerization. Curr. Opin. Pharmacol. 10, 44-52
8. Cottet, M., Faklaris, O., Maurel, D., Scholler, P., Doumazane, E., Trinquet, E., Pin, J. P. and Durroux, T. (2012) BRET and Time-resolved FRET strategy to study GPCR oligomerization: from cell lines toward native tissues. Front. Endocrinol. 3, 92
9. Lohse, M. J., Nuber, S. and Hoffmann, C. (2012) Fluorescence/ bioluminescence resonance energy transfer techniques to study G-protein-coupled receptor activation and signaling. Pharmacol. Rev. 64, 299-336
10. Selvin, P. R. (2002) Principles and biophysical applications of lanthanide-based probes. Annu. Rev. Biophys. Biomol. Struct. 31, 275-302 (Pubitemid 34666834)
11. Bazin, H., Trinquet, E. and Mathis, G. (2002) Time resolved amplification of cryptate emission: a versatile technology to trace biomolecular interactions. J. Biotechnol. 82, 233-250 (Pubitemid 34179603)
12. Mathis, G. (1995) Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer. Clin. Chem. 41, 1391-1397
13. Xu, J., Corneillie, T. M., Moore, E. G., Law, G. L., Butlin, N. G. and Raymond, K. N. (2011) Octadentate cages of Tb (III) 2-hydroxyisophthalamides: a new standard for luminescent lanthanide labels. J. Am. Chem. Soc. 133, 19900-19910
14. Cottet, M., Faklaris, O., Zwier, J. M., Trinquet, E., Pin, J.-P. and Durroux, T. (2011) Original fluorescent ligand-based assays open new perspectives in G-protein coupled receptor drug screening pharmaceuticals. 4, 202-214
15. Albizu, L., Teppaz, G., Seyer, R., Bazin, H., Ansanay, H., Manning, M., Mouillac, B. and Durroux, T. (2007) Toward efficient drug screening by homogeneous assays based on the development of new fluorescent vasopressin and oxytocin receptor ligands. J. Med. Chem. 50, 4976-4985 (Pubitemid 47527131)
16. Owicki, J. C. (2000) Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer. J. Biomol. Screening 5, 297-306
17. Abel, A., Wittau, N., Wieland, T., Schultz, G. and Kalkbrenner, F. (2000) Cell cycle-dependent coupling of the vasopressin V1a receptor to different G proteins. J. Biol. Chem. 275, 32543-32551
18. Albers, H. E., Karom, M. and Smith, D. (2002) Serotonin and vasopressin interact in the hypothalamus to control communicative behavior. NeuroReport 13, 931-933 (Pubitemid 34518211)
19. Allen, M., Reeves, J. and Mellor, G. (2000) High throughput fluorescence polarization: a homogeneous alternative to radioligand binding for cell surface receptors. J. Biomol. Screening 5, 63-69 (Pubitemid 30243875)
20. Ilien, B., Franchet, C., Bernard, P., Morisset, S., Weill, C. O., Bourguignon, J. J., Hibert, M. and Galzi, J. L. (2003) Fluorescence resonance energy transfer to probe human M1 muscarinic receptor structure and drug binding properties. J. Neurochem. 85, 768-778 (Pubitemid 36531940)
21. Bonnet, B., Ilien, B., Galzi, J. L., Riche, S., Antheaune, C. and Hibert, M. (2006) A rapid and versatile method to label receptor ligands using "click" chemistry: Validation with the muscarinic M1 antagonist pirenzepine. Bioconjugate Chem. 17, 1618-1623 (Pubitemid 44851510)
22. Zwier, J. M., Roux, T., Cottet, M., Durroux, T., Douzon, S., Bdioui, S., Gregor, N., Bourrier, E., Oueslati, N., Nicolas, L. et al. (2010) A fluorescent ligand-binding alternative using Tag-lite® technology. J. Biomol. Screening 15, 1248-1259
23. Maurel, D., Kniazeff, J., Mathis, G., Trinquet, E., Pin, J. P. and Ansanay, H. (2004) Cell surface detection of membrane protein interaction with homogeneous time-resolved fluorescence resonance energy transfer technology. Anal. Biochem. 329, 253-262 (Pubitemid 38680506)
24. Gronemeyer, T., Chidley, C., Juillerat, A., Heinis, C. and Johnsson, K. (2006) Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling. Protein Eng Des Sel 19, 309-316 (Pubitemid 43999641)
25. Juillerat, A., Gronemeyer, T., Keppler, A., Gendreizig, S., Pick, H., Vogel, H. and Johnsson, K. (2003) Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo. Chem. Biol. 10, 313-317 (Pubitemid 36516649)
26. Juillerat, A., Heinis, C., Sielaff, I., Barnikow, J., Jaccard, H., Kunz, B., Terskikh, A. and Johnsson, K. (2005) Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells. ChemBioChem 6, 1263-1269 (Pubitemid 40978188)
27. Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H. and Johnsson, K. (2003) A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat. Biotechnol. 21, 86-89 (Pubitemid 36055833)
28. Keppler, A., Pick, H., Arrivoli, C., Vogel, H. and Johnsson, K. (2004) Labeling of fusion proteins with synthetic fluorophores in live cells. Proc. Natl. Acad. Sci. U. S. A. 101, 9955-9959 (Pubitemid 38891177)
29. Gautier, A., Juillerat, A., Heinis, C., Correa, I. R., Jr., Kindermann, M., Beaufils, F. and Johnsson, K. (2008) An engineered protein tag for multiprotein labeling in living cells. Chem. Biol. 15, 128-136 (Pubitemid 351253620)
30. Comps-Agrar, L., Kniazeff, J., Norskov-Lauritsen, L., Maurel, D., Gassmann, M., Gregor, N., Prezeau, L., Bettler, B., Durroux, T., Trinquet, E. and Pin, J. P. (2011) The oligomeric state sets GABA (B) receptor signalling efficacy. EMBO J. 30, 2336-2349
31. Doumazane, E., Scholler, P., Zwier, J. M., Eric, T., Rondard, P. and Pin, J. P. (2011) A new approach to analyze cell surface protein complexes reveals specific heterodimeric metabotropic glutamate receptors. FASEB J. 25, 66-77
32. Maurel, D., Comps-Agrar, L., Brock, C., Rives, M. L., Bourrier, E., Ayoub, M. A., Bazin, H., Tinel, N., Durroux, T., Prezeau, L. et al. (2008) Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies: application to GPCR oligomerization. Nat. Methods 5, 561-567 (Pubitemid 351761765)
33. a Bouvier, M. (2001) Oligomerization of G-protein-coupled transmitter receptors. Nat. Rev. Neurosci. 2, 274-286 (Pubitemid 33673068)
34. Terrillon, S. and Bouvier, M. (2004) Roles of G-protein-coupled receptor dimerization. EMBO Rep. 5, 30-34 (Pubitemid 38228524)
35. Milligan, G. (2010) The role of dimerisation in the cellular trafficking of G-protein-coupled receptors. Curr. Opin. Pharmacol. 10, 23-29
36. Guo, W., Urizar, E., Kralikova, M., Mobarec, J. C., Shi, L., Filizola, M. and Javitch, J. A. (2008) Dopamine D2 receptors form higher order oligomers at physiological expression levels. EMBO J. 27, 2293-2304
37. Fotiadis, D., Jastrzebska, B., Philippsen, A., Muller, D. J., Palczewski, K. and Engel, A. (2006) Structure of the rhodopsin dimer: a working model for G-protein-coupled receptors. Curr. Opin. Struct. Biol. 16, 252-259
38. Fotiadis, D., Liang, Y., Filipek, S., Saperstein, D. A., Engel, A. and Palczewski, K. (2003) Atomic-force microscopy: Rhodopsin dimers in native disc membranes. Nature 421, 127-128 (Pubitemid 36090969)
39. Albizu, L., Cottet, M., Kralikova, M., Stoev, S., Seyer, R., Brabet, I., Roux, T., Bazin, H., Bourrier, E., Lamarque, L. et al. (2010) Time-resolved FRET between GPCR ligands reveals oligomers in native tissues. Nat. Chem. Biol. 6, 587-594
40. Albizu, L., Balestre, M. N., Breton, C., Pin, J. P., Manning, M., Mouillac, B., Barberis, C. and Durroux, T. (2006) Probing the existence of G protein-coupled receptor dimers by positive and negative ligand-dependent cooperative binding. Mol. Pharmacol. 70, 1783-1791