Sex selection of sperm in farm animals: Status report and developmental prospects

Reproduction

Volumn 145, Issue 1, 2013, Pages

  Rath, D ^a   Barcikowski, S ^b   Graaf, S De ^c   Garrels, W ^a   Grossfeld, R ^a   Klein, S ^a   Knabe, W ^d   Knorr, C ^e   Kues, W ^a   Meyer, H ^f   Michl, J ^e   Moench Tegeder, G ^a   Rehbock, C ^b   Taylor, U ^a   Washausen, S ^d  

^a Institute of Farm Animal Genetics   (Germany)

^b UNIVERSITY OF DUISBURG ESSEN   (Germany)

^c UNIVERSITY OF SYDNEY   (Australia)

^d UNIVERSITY OF MÜNSTER   (Germany)

^e UNIVERSITY OF GÖTTINGEN   (Germany)

^f LASER ZENTRUM HANNOVER E V   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GOLD NANOPARTICLE;

FARM ANIMAL; FERTILIZATION IN VITRO; HYBRIDIZATION; INTERNALIZATION; LASER; LIFESPAN; NANOTECHNOLOGY; NONHUMAN; OVIDUCT; PRIORITY JOURNAL; REVIEW; SEX DETERMINATION; SEX SELECTION; SPERM; SPERMATOZOON; STATIC ELECTRICITY; Y CHROMOSOME;

ANIMALS; ANIMALS, DOMESTIC; BREEDING; CATTLE; DNA; FEMALE; FLOW CYTOMETRY; INSEMINATION, ARTIFICIAL; MALE; METAL NANOPARTICLES; SEX PRESELECTION; SHEEP, DOMESTIC; SPERMATOZOA; X CHROMOSOME; Y CHROMOSOME;

EID: 84873030229     PISSN: 14701626     EISSN: 17417899     Source Type: Journal    
DOI: 10.1530/REP-12-0151     Document Type: Review

References (170)

1. Abeydeera LR, Johnson LA, Welch GR, Wang WH, Boquest AC, Cantley TC, Rieke A & Day BN 1998 Birth of piglets preselected for gender following in vitro fertilization of in vitro matured pig oocytes by X and Y chromosome bearing spermatozoa sorted by high speed flow cytometry. Theriogenology 50 981-988. (doi:10.1016/S0093-691X (98)00201-5).
2. Aitken RJ, Clarkson JS & Fishel S 1989 Generation of reactive oxygen species, lipid peroxidation, and human sperm function. Biology of Reproduction 41 183-197. (doi:10.1095/biolreprod41.1.183).
3. Alvarez JG, Touchstone JC, Blasco L & Storey BT 1987 Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity. Journal of Andrology 8 338-348.
4. André J 1962 Contribution à la connaissance du chondriome. Journal of Ultrastructure Research 6 (Supplement 1) 1-185. (doi:10.1016/S0889- 1605(62)80002-0).
5. Arvizo RR, Miranda OR, Thompson MA, Pabelick CM, Bhattacharya R, Robertson JD, Rotello VM, Prakash YS & Mukherjee P 2010 Effect of nanoparticle surface charge at the plasma membrane and beyond. Nano Letters 10 2543-2548. (doi:10.1021/nl101140t).
6. Balao da Silva C, Macias-Garcia B, Morillo Rodriguez A, Gallardo Bolanos JM, Tapia JA, Aparicio IM, Morrell JM, Rodriguez- Martinez H, Ortega-Ferrusola C & Pena FJ 2012 Effect of Hoechst 33342 on stallion spermatozoa incubated in KMT or tyrodes modified INRA96. Animal Reproduction Science 131 165-171. (doi:10.1016/j. anireprosci.2012.01.003).
7. Balasubramanian SK, Jittiwat J, Manikandan J, Choon-Nam O, Liya EY & Wei-Yi O 2010 Biodistribution of gold nanoparticles and gene expression changes in the liver and spleen after intravenous administration in rats. Biomaterials 31 2034-2042. (doi:10.1016/j.biomaterials. 2009.11.079).
8. Barcelo-Fimbres M, Campos-Chillon LF & Seidel GE Jr 2011 In vitro fertilization using non-sexed and sexed bovine sperm: sperm concentration, sorter pressure, and bull effects. Reproduction in Domestic Animals 46 495-502. (doi:10.1111/j.1439-0531.2010.01696.x).
9. Bar-Ilan O, Albrecht RM, Fako VE & Furgeson DY 2009 Toxicity assessments of multi-sized gold and silver nanoparticles in zebrafish embryos. Small 16 1897-1910. (doi:10.1002/smll.200801716).
10. Beilby KH, Grupen CG, Thomson PC, Maxwell WMC & Evans G 2009 The effect of insemination time and sperm dose on pregnancy rate using sex-sorted ram sperm. Theriogenology 71 829-835. (doi:10.1016/j.theriogenology.2008.10.005) .
11. Beilby KH, de Graaf SP & Grupen CG 2010 The effect of sperm and cryoprotectant concentration on the freezing success of sex sorted ram sperm for in vitro fertilization. Theriogenology 74 786-794. (doi:10.1016/j. theriogenology.2010.04.003).
12. Beilby KH, de Graaf SP, Evans G, Maxwell WMC, Wilkening S, Wrenzycki C & Grupen CG 2011 Quantitative mRNA expression in ovine blastocysts produced from X- and Y-chromosome bearing sperm, both in vitro and in vivo. Theriogenology 76 471-481. (doi:10.1016/j. theriogenology.2011.02.024).
13. Bermejo-Alvarez P, Rizos D, Rath D, Lonergan P & Gutierrez-Adan A 2008 Can bovine in vitro-matured oocytes selectively process X- or Y-sorted sperm differentially? Biology of Reproduction 79 594-597. (doi:10.1095/ biolreprod.108.070169).
14. Bermejo-Alvarez P, Lonergan P, Rath D, Gutierrez-Adan A & Rizos D 2010 Developmental kinetics and gene expression in male and female bovine embryos produced in vitro with sex-sorted spermatozoa. Reproduction, Fertility, and Development 22 426-436. (doi:10.1071/RD09142).
15. Berry CC, de la Fuente JM, Mullin M, Chu SWL & Curtis ASG 2007 Nuclear localization of HIV-1 tat functionalized gold nanoparticles. IEEE Transactions on Nanobioscience 6 262-269. (doi:10.1109/TNB.2007. 908973).
16. Beyhan Z, Johnson LA & First NL 1999 Sexual dimorphism in IVM-IVF bovine embryos produced from X and Y chromosome-bearing spermatozoa sorted by high speed flow cytometry. Theriogenology 52 35-48. (doi:10.1016/S0093-691X(99) 00108-9).
17. Bonde JP 2010 Male reproductive organs are at risk from environmental hazards. Asian Journal of Andrology 12 152-156. (doi:10.1038/aja. 2009.83).
18. Buchanan BR, Seidel GE Jr, McCue PM, Schenk JL, Herickhoff LA & Squires EL 2000 Insemination of mares with low numbers of either unsexed or sexed spermatozoa. Theriogenology 53 1333-1344. (doi:10.1016/S0093-691X(00) 00276-4).
19. Buchini S & Leumann CJ 2003 New nucleoside analogues for the recognition of pyrimidine-purine inversion sites. Nucleosides, Nucleotides & Nucleic Acids 22 1199-1201. (doi:10.1081/NCN-120022835).
20. Carvalho JO, Sartori R, Machado GM, Mourão GB & Dode MA 2010 Quality assessment of bovine cryopreserved sperm after sexing by flow cytometry and their use in in vitro embryo production. Theriogenology 74 1521-1530. (doi:10.1016/j.theriogenology.2010.06.030).
21. Carvalho JO, Michalczechen-Lacerda VA, Sartori R, Rodrigues FC, Bravim O, Franco MM & Dode MA 2012 The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting. Molecular Reproduction and Development 79 77-84. (doi:10.1002/mrd.21410).
22. Chinoy NJ, Sequeira E & Narayana MV 1991 Effects of vitamin C and calcium on the reversibility of fluoride-induced alterations in spermatozoa of rabbits. Fluoride 24 29-39.
23. Chithrani BD & Chan WCW 2007 Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes. Nano Letters 7 1542-1550. (doi:10.1021/nl070363y).
24. Chithrani BD, Ghazani AA & Chan WCW 2006 Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Letters 6 662-668. (doi:10.1021/nl052396o).
25. Clulow JR, Mansfield LJ, Morris LH, Evans G & Maxwell WM 2008 A comparison between freezing methods for the cryopreservation of stallion spermatozoa. Animal Reproduction Science 108 298-308. (doi:10.1016/j. anireprosci.2007.08.014).
26. Clulow JR, Evans G, Morris LH & Maxwell WM 2009 Factors influencing the "sortability" of stallion spermatozoa into X- and Y-chromosome bearing populations. Animal Reproduction Science 113 220-228. (doi:10.1016/j.anireprosci.2008.08.019).
27. Cran DG & Johnson LA 1996 The predetermination of embryonic sex using flow cytometrically separated X and Y spermatozoa. Human Reproduction Update 2 355-363. (doi:10.1093/humupd/2.4.355).
28. Cran DG, Johnson LA, Miller NG, Cochrane D & Polge C 1993 Production of bovine calves following separation of X- and Y-chromosome bearing sperm and in vitro fertilisation. Veterinary Record 132 40-41. (doi:10.1136/vr.132.2.40).
29. De Ambrogi M, Spinaci M, Galeati G & Tamanini C 2006 Viability and DNAfragmentation in differently sorted boar spermatozoa. Theriogenology 66 1994-2000. (doi:10.1016/j.theriogenology.2006.05.017).
30. Dean PN, Pinkel D & Mendelsohn ML 1978 Hydrodynamic orientation of sperm heads for flow cytometry. Biophysical Journal 23 7-13. (doi:10.1016/S0006-3495(78)85428-9).
31. De Cecco M, Spinaci M, Zannoni A, Bernardini C, Seren E, Forni M & Bacci ML 2010 Coupling sperm mediated gene transfer and sperm sorting techniques: a new perspective for swine transgenesis. Theriogenology 74 856-862. (doi:10.1016/j.theriogenology.2010.04.010).
32. DeJarnette JM, Nebel RL & Marshall CE 2009 Evaluating the success of sexsorted semen in U.S. dairy herds from on farm records. Theriogenology 71 49-58. (doi:10.1016/j.theriogenology.2008.09.042).
33. Dejarnette JM, Leach MA, Nebel RL, Marshall CE, McCleary CR & Moreno JF 2011 Effects of sex-sorting and sperm dosage on conception rates of Holstein heifers: is comparable fertility of sex-sorted and conventional semen plausible? Journal of Dairy Science 94 3477-3483. (doi:10.3168/jds.2011-4214).
34. De Jong WH, Hagens WI, Krystek P, Burger MC, Sips AJ & Gertsma RE 2008 Particle size dependent organ distribution of gold nanoparticles after intravenous administration. Biomaterials 29 1912-1919. (doi:10.1016/j. biomaterials.2007.12.037).
35. De Martino C, Floridi A, Marcante M, MalorniW, Barcellona P, Bellocci M & Silvestrini B 1979 Morphological, histochemical and biochemical studies on germ cell mitochondria of normal rats. Cell and Tissue Research 196 1-22.
36. Den Daas JH, De Jong G, Lansbergen LM & Van Wagtendonk-De Leeuw AM 1998 The relationship between the number of spermatozoa inseminated and the reproductive efficiency of individual dairy bulls. Journal of Dairy Science 81 1714-1723. (doi:10.3168/jds.S0022-0302 (98)75739-X).
37. Derossi D, Joliot AH, Chassaing G & Prochiantz A 1994 The third helix of the antennapedia homeodomain translocates through biological membranes. Journal of Biological Chemistry 269 10444-10450.
38. De Vries A, Overton M, Fetrow J, Leslie K, Eicker S & Rogers G 2008 Exploring the impact of sexed semen on the structure of the dairy industry. Journal of Dairy Science 91 847-856. (doi:10.3168/jds.2007-0536).
39. Ema M, Kobayashi N, Naya M, Hanai S & Nakanishi J 2010 Reproductive and developmental toxicity studies of manufactured nanomaterials. Reproductive Toxicology 30 343-352. (doi:10.1016/j.reprotox.2010.06. 002).
40. Everts M, Saini V, Leddon JL, Kok RJ, Stoff-Khalili M, Preuss MA, Millican CL, Perkins G, Brown JM, Bagaria H et al. 2006 Covalently linked Au nanoparticles to a viral vector: potential for combined photothermal and gene cancer therapy. Nano Letters 6 587-591. (doi:10.1021/nl0500555).
41. de la Fuente JM & Berry CC 2005 Tat peptide as an efficient molecule to translocate gold nanoparticles into the cell nucleus. Bioconjugate Chemistry 16 1176-1180. (doi:10.1021/bc050033C).
42. Fulwyler MJ 1977 Hydrodynamic orientation of cells. Journal of Histochemistry and Cytochemistry 25 781-783. (doi:10.1177/25.7.330728).
43. Gagne MB, Pothier F & Sirard MA 1991 Electroporation of bovine spermatozoa to carry foreign DNA in oocytes. Molecular Reproduction and Development 29 6-15. (doi:10.1002/mrd.1080290103).
44. Gao HJ, Shi WD & Freund LB 2005 Mechanics of receptor-mediated endocytosis. PNAS 102 9469-9474. (doi:10.1073/pnas.0503879102).
45. Garner DL 2009 Hoechst 33342: the dye that enabled differentiation of living X-and Y-chromosome bearing mammalian sperm. Theriogenology 71 11-21. (doi:10.1016/j.theriogenology.2008.09.023).
46. Garner DL, Gledhill BL, Pinkel D, Lake S, Stephenson D, Van Dilla MA & Johnson LA 1983 Quantification of the X- and Y-chromosome-bearing spermatozoa of domestic animals by flow cytometry. Biology of Reproduction 28 312-321. (doi:10.1095/biolreprod28.2.312).
47. Garrels W, Holler S, Taylor U, Herrmann D, Struckmann C, Klein S, Barg-Kues B, Nowak-Imialek M, Ehling C, Rath D et al. 2011 Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa. PLoS ONE 6 e27563. (doi:10.1371/journal.pone. 0027563).
48. Gibb Z, Morris LH, Maxwell WM & Grupen CG 2011 Use of a defined diluent increases the sex-sorting efficiency of stallion sperm. Theriogenology 75 610-619. (doi:10.1016/j.theriogenology.2010.10.001).
49. Gledhill BL, Pinkel D, Garner DL & Van Della MA 1982 Identifying X- and Y-chromosome-bearing sperm by DNA content: retrospective perspectives and prospective opinions. In Prospects for Sexing Mammalian Sperm, pp 177-191. Eds RP Amann& GE Seidel. Boulder, CO, USA: Colorado Associated University Press.
50. de Graaf SP, Evans G, MaxwellWMC & O'Brien JK 2006 In vitro function of fresh and frozen-thawed ram spermatozoa after sex-sorting and re-freezing. Reproduction, Fertility, and Development 18 867-874. (doi:10.1071/RD06061).
51. de Graaf SP, Beilby KH, O'Brien JK, Osborn D, Downing JA, Maxwell WMC & Evans G 2007a Embryo production from superovulated sheep inseminated with sex-sorted ram spermatozoa. Theriogenology 67 550-555. (doi:10.1016/j. theriogenology.2006.09.002).
52. de Graaf SP, Evans G, Maxwell WMC, Cran DG & O'Brien JK 2007b Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa. Theriogenology 67 391-398. (doi:10.1016/j.theriogenology.2006.08. 005).
53. de Graaf SP, Evans G, Maxwell WMC, Downing JA & O'Brien JK 2007c Successful low dose insemination of flow cytometrically sorted ram spermatozoa in sheep. Reproduction in Domestic Animals 42 648-653. (doi:10.1111/j.1439-0531. 2006.00837.x).
54. de Graaf SP, Beilby KH, Underwood SL, Evans G & Maxwell WMC 2009 Sperm sexing in sheep and cattle: the exception and the rule. Theriogenology 71 89-97. (doi:10.1016/j.theriogenology.2008.09.014).
55. Green M & Loewenstein PM 1988 Autonomous functional domains of chemically synthesized human immunodeficiency virus tat transactivator protein. Cell 55 1179-1188. (doi:10.1016/0092-8674(88)90262-0).
56. Grossfeld R, Klinc P, Sieg B & Rath D 2005 Production of piglets with sexed semen employing a non-surgical insemination technique. Theriogenology 63 2269-2277. (doi:10.1016/j.theriogenology.2004.10.004).
57. Grossfeld R, Strothmeyer M & Rath D 2011 Low dose sperm intra fallopian transfer (SIFT) in dairy cows. Reproduction in Domestic Animals 46 (Suppl 3) 109 (Abstr). (doi:10.1111/j.1439-0531.2011.01839.x).
58. Guthrie HD, Johnson LA, Garrett WM, Welch GR & Dobrinsky JR 2002 Flow cytometric sperm sorting: effects of varying laser power on embryo development in swine. Molecular Reproduction and Development 61 87-92. (doi:10.1002/mrd. 1134).
59. Hayakawa H, Hirai T, Takimoto A, Ideta A & Aoyagi Y 2009 Superovulation and embryo transfer in Holstein cattle using sexed sperm. Theriogenology 71 68-73. (doi:10.1016/j.theriogenology.2008.09.016).
60. Heer P 2007 Anpassung der Konservierungsprozesse fuer Hengstsperma an die Beltsville Sperm Sexing Technology [Adjustment of the cryopreservation of stallion spermatozoa to the Beltsville Sperm Sexing Technology]. PhD Thesis. University of Veterinary Medicine Hannover, Germany.
61. Hollinshead FK, O'Brien JK, Maxwell WMC & Evans G 2002 Production of lambs of predetermined sex after the insemination of ewes with low numbers of frozen-thawed sorted X- or Y-chromosome-bearing spermatozoa. Reproduction, Fertility, and Development 14 503-508. (doi:10.1071/RD02034).
62. Hollinshead FK, Gillan L, O'Brien JK, Evans G & Maxwell WMC 2003 In vitro and in vivo assessment of functional capacity offlow cytometrically sorted ramspermatozoa after freezing and thawing. Reproduction, Fertility, and Development 15 351-359. (doi:10.1071/RD03060).
63. Hollinshead FK, Evans G, Evans KM, Catt SL, Maxwell WMC & O'Brien JK 2004 Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen-thawed sex-sorted and re-frozen-thawed ram spermatozoa. Reproduction 127 557-568. (doi:10.1530/rep.1.00049).
64. Hoogsteen K 1963 Crystal and molecular structure of a hydrogen-bonded complex between 1-methylthymine and 9-methyladenine. Acta Crystallographica 16 907-916. (doi:10.1107/S0365110X63002437).
65. Jain PK, El-Sayed IH & El-Sayed MA 2007 Au nanoparticles target cancer. Nano Today 2 18-29. (doi:10.1016/S1748-0132(07)70016-6).
66. Jiang W, Kim BYS, Rutka JT & Chan WCW 2008 Nanoparticle-mediated cellular response is size-dependent. Nature Nanotechnology 3 145-150. (doi:10.1038/nnano.2008.30).
67. Johnson LA & Pinkel D 1986 Modification of a laser-based flow cytometer for high-resolution DNA analysis of mammalian spermatozoa. Cytometry 7 268-273. (doi:10.1002/cyto.990070307).
68. Johnson LA & Clarke RN 1988 Flow sorting of X and Y chromosomebearing mammalian sperm: activation and pronuclear development of sorted bull, boar, and ram sperm microinjected into hamster oocytes. Gamete Research 21 335-343. (doi:10.1002/mrd.1120210402).
69. Johnson MD & Fresco JR 1999a Third-strand in situ hybridization (TISH) to non-denatured metaphase spreads and interphase nuclei. Chromosoma 108 181-189. (doi:10.1007/s004120050367).
70. Johnson LA & Welch GR 1999b Sex preselection: high-speed flow cytometric sorting of X and Y sperm for maximum efficiency. Theriogenology 52 1323-1341. (doi:10.1016/S0093-691X(99)00220-4).
71. Johnson LA, Flook JP & Look MV 1987a Flow cytometry of X and Y chromosome-bearing sperm for DNA using an improved preparation method and staining with Hoechst 33342. Gamete Research 17 203-212. (doi:10.1002/mrd. 1120170303).
72. Johnson LA, Flook JP, Look MV & Pinkel D 1987b Flow sorting of X and Y chromosome-bearing spermatozoa into two populations. Gamete Research 16 1-9. (doi:10.1002/mrd.1120160102).
73. Johnson LA, Welch GR & Rens W 1999 The Beltsville Sperm Sexing Technology: high-speed sperm sorting gives improved sperm output for in vitro fertilization and AI. Journal of Animal Science 77 (Suppl 2) 213-220.
74. Kawarasaki T, Welch GR, Long CR, Yoshida M & Johnson LA 1998 Verification of flow cytometrically-sorted X- and Y-bearing porcine spermatozoa and reanalysis of spermatozoa for DNA content using the fluorescence in situ hybridization (FISH) technique. Theriogenology 50 625-635. (doi:10.1016/S0093- 691X(98)00167-8).
75. Klein S, Petersen S, Taylor U, Barcikowski S & Rath D 2010 Quantitative visualization of colloidal and intracellular gold nanoparticles by confocal microscopy. Journal of Biomedical Optics 15 036015. (doi:10.1117/1.3461170).
76. Klinc P 2005 Improved fertility of flowcytometrically sex selected bull spermatozoa. PhD Thesis. University of Veterinary Medicine Hannover, Germany.
77. Klinc P & Rath D 2007 Reduction of oxidative stress in bovine spermatozoa during flow cytometric sorting. Reproduction in Domestic Animals 42 63-67. (doi:10.1111/j.1439-0531.2006.00730.x).
78. Klinc P, Frese D, Osmers H & Rath D 2007 Insemination with sex sorted fresh bovine spermatozoa processed in the presence of antioxidative substances. Reproduction in Domestic Animals 42 58-62. (doi:10.1111/j.1439-0531.2006.00729. x).
79. Krueger C & Rath D 2000 Intrauterine insemination in sows with reduced sperm number. Reproduction, Fertility, and Development 12 113-117. (doi:10.1071/RD00035).
80. KuesW, Sudheer S, Herrmann D, Carnwath JW, Havlicek V, Besenfelder U, Lehrach H, Adjaye J & Niemann H 2008a Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo. PNAS 105 19768-19773. (doi:10.1073/pnas. 0805616105).
81. Kues W, Rath D & Niemann H 2008b Reproductive biotechnology in farm animals goes genomics. CAB Reviews 3 036. (doi:10.1079/PAVS NNR20083036).
82. de Lamirande E, Jiang H, Zini A, Kodama H & Gagnon C 1997 Reactive oxygen species and sperm physiology. Reviews of Reproduction 2 48-54.
83. Leahy T, Celi P, Bathgate R, Evans G, Maxwell WM & Marti JI 2010 Flow-sorted ram spermatozoa are highly susceptible to hydrogen peroxide damage but are protected by seminal plasma and catalase. Reproduction, Fertility, and Development 22 1131-1140. (doi:10.1071/RD09286).
84. Lindsey AC, Morris LH, Allen WR, Schenk JL, Squires EL & Bruemmer JE 2002 Hysteroscopic insemination of mares with low numbers of nonsorted or flow sorted spermatozoa. Equine Veterinary Journal 34 128-132. (doi:10.2746/ 042516402776767178).
85. Liu HT, Niu RY, Wang JM, He Y & Wang JD 2008 Changes caused by fluoride and lead in energy metabolic enzyme activities in the reproductive system of male offspring rats. Flouride 41 184-191.
86. Lu KH, Cran DG & Seidel GE Jr 1999 In vitro fertilization with flowcytometrically- sorted bovine sperm. Theriogenology 52 1393-1405. (doi:10.1016/S0093-691X(99)00225-3).
87. Lundberg M & Johansson M 2002 Positively charged DNA-binding proteins cause apparent cell membrane translocation. Biochemical and Biophysical Research Communications 291 367-371. (doi:10.1006/bbrc.2002.6450).
88. Mandal D, Maran A, Yaszemski MJ, Bolander ME & Sarkar G 2009 Cellular uptake of gold nanoparticles directly cross-linked with carrier peptides by osteosarcoma cells. Journal of Materials Science: Materials in Medicine 20 347-350. (doi:10.1007/s10856-008-3588-x).
89. Martinez EA, Vazquez JM, Roca J, Lucas X, GilMA & Vazquez JL 2001 Deep intrauterine insemination and embryo transfer in pigs. Reproduction Supplement 58 301-311.
90. Maxwell WM & Johnson LA 1997 Chlortetracycline analysis of boar spermatozoa after incubation, flow cytometric sorting, cooling, or cryopreservation. Molecular Reproduction and Development 46 408-418. (doi:10.1002/(SICI)1098-2795(199703)46:3!408::AID-MRD21 O3.0.CO;2-T).
91. McKenzie F, Faulds K & Graham D 2008 LNA functionalized gold nanoparticles as probes for double strandedDNA through triplex formation. Chemical Communications 20 2367-2369. (doi:10.1039/b802163e).
92. Meinhardt A, McFarlane JR, Seitz J & de Kretser DM 2000 Activin maintains the condensed type of mitochondria in germ cells. Molecular and Cellular Endocrinology 168 111-117. (doi:10.1016/S0303-7207 (00)00308-7).
93. Merton JS, Haring RM, Stap J, Hoebe RA & Aten JA 1997 Effect of flow cytometrically sorted sperm on success rate of in vitro bovine embryo production. Theriogenology 47 295. (doi:10.1016/S0093-691X (97)82422-3).
94. Moench-Tegeder G 2008 Auswirkungen verschiedener spermaaufbereitungen auf die lebensfähigkeit geschlechtsspezifisch differenzierter spermatozoen. Masters Thesis. Faculty of Agricultural Sciences, University of Gö ttingen, Germany.
95. Moench-Tegeder G 2011 Einfluss Verschiedener Ejakulatbehandlungen auf die Lebensfähigkeit und das Befruchtungspotential Geschlechtsspezifisch Differenzierter Bullenspermien [Effect of different ejaculate treatments on viability and fertilizing capacity of sex sorted bull spermatozoa]. PhD Thesis. Faculty of Agricultural Sciences, University of Gö ttingen, Germany.
96. Morrell JM, Keeler KD, Noakes DE, Mackenzie NM & Dresser DW 1988 Sexing of sperm by flow cytometry. Veterinary Record 122 322-324. (doi:10.1136/vr.122.14.322).
97. Morris LH, Hunter RH & Allen WR 2000 Hysteroscopic insemination of small numbers of spermatozoa at the uterotubal junction of preovulatory mares. Journal of Reproduction and Fertility 118 95-100. (doi:10.1530/reprod/118.1.95).
98. Morton KM, Bathgate R, Evans G & Maxwell WM 2007a Cryopreservation of epididymal alpaca (Vicugna pacos) sperm: a comparison of citrate-, tris- and lactose-based diluents and pellets and straws. Reproduction, Fertility, and Development 19 792-796. (doi:10.1071/RD07049).
99. Morton KM, Herrmann D, Sieg B, Struckmann C, Maxwell WM, Rath D, Evans G, Lucas-Hahn A, Niemann H & Wrenzycki C 2007b Altered mRNA expression patterns in bovine blastocysts after fertilisation in vitro using flow-cytometrically sex-sorted sperm. Molecular Reproduction and Development 74 931-940. (doi:10.1002/mrd.20573).
100. van Munster EB 2002 Interferometry in flow to sort unstained X- and Y-chromosome-bearing bull spermatozoa. Cytometry 47 192-199. (doi:10.1002/cyto.10064).
101. Nativo P, Prior IA & Brust M 2008 Uptake and intracellular fate of surfacemodified gold nanoparticles. ACS Nano 2 1639-1644. (doi:10.1021/ nn800330a).
102. Nel AE, Madler L, Velegol D, Xia T, Hoek EMV, Somasundaran P, Klaessig F, Castranova V & Thompson M 2009 Understanding biophysicochemical interactions at the nano-bio interface. Nature Materials 8 543-557. (doi:10.1038/nmat2442).
103. Niemann H, Kuhla B & Flachowsky G 2011 Perspectives for feedefficient animal production. Journal of Animal Science 89 4344-4363. (doi:10.2527/jas. 2011-4235).
104. Norman HD, Hutchison JL & Miller RH 2010 Use of sexed semen and its effect on conception rate, calf sex, dystocia, and stillbirth of Holstein in the United States. Journal of Dairy Science 93 3880-3890. (doi:10.3168/jds.2009- 2781).
105. O'Brien JK, Hollinshead FK, Evans KM, Evans G & Maxwell WMC 2003 Flow cytometric sorting of frozen-thawed spermatozoa in sheep and non-human primates. Reproduction, Fertility, and Development 15 367-375. (doi:10.1071/RD03065).
106. O'Brien JK, Steinman KJ & Robeck TR 2009 Application of sperm sorting and associated reproductive technology for wildlife management and conservation. Theriogenology 71 98-107. (doi:10.1016/j.theriogenology. 2008.09.052).
107. Okunade GW, Miller ML, Pyne GJ, Sutliff RL, O'Connor KT, Neumann JC, Andringa A, Miller DA, Prasad V, Doetschman T et al. 2004 Targeted ablation of plasma membrane Ca2C-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. Journal of Biological Chemistry 279 33742-33750. (doi:10.1074/jbc.M40462 8200).
108. Palma GA, Olivier NS, Neumuller C & Sinowatz F 2008 Effects of sex-sorted spermatozoa on the efficiency of in vitro fertilization and ultrastructure of in vitro produced bovine blastocysts. Anatomia, Histologia, Embryologia 37 67-73.
109. Pelliccione F, Micillo A, Cordeschi G, D'Angeli A, Necozione S, Gandini L, Lenzi A, Francavilla F & Francavilla S 2011 Altered ultrastructure of mitochondrial membranes is strongly associated with unexplained asthenozoospermia. Fertility and Sterility 95 641-646. (doi:10.1016/j. fertnstert.2010.07.1086).
110. Petersen S, Barchanski A, Taylor U, Klein S, Rath D & Barcikowski S 2011 Penetratin-conjugated gold nanoparticles: design of cell penetrating nanomarker by laser ablation. Bioconjugate Chemistry 115 5152-5159. (doi:10.1021/jp1093614).
111. Pinkel D, Gledhill BL, Lake S, Stephenson D & Van Dilla MA 1982 Sex preselection in mammals? Separation of sperm bearing Y and "O" chromosomes in the vole Microtus oregoni Science 218 904-906. (doi:10.1126/science.6753153).
112. Piomboni P, Focarelli R, Stendardi A, Ferramosca A & Zara V 2012 The role of mitochondria in energy production for human sperm motility. International Journal of Andrology 35 109-124. (doi:10.1111/j.1365- 2605.2011.01218.x).
113. Probst S & Rath D 2003 Production of piglets using intracytoplasmic sperm injection (ICSI) with flowcytometrically sorted boar semen and artificially activated oocytes. Theriogenology 59 961-973. (doi:10.1016/S0093- 691X(02)01135-4).
114. Rahmy TR & Ayoub MA 2002 In vitro action of cobra venom on goat spermatozoa ultrastructure by transmission and scanning electron microscopy. Journal of Venomous Animals and Toxins 8 127-159. (doi:10.1590/S0104- 79302002000100011).
115. Rath D & Johnson LA 2008 Application and commercialization of flow cytometrically sex-sorted semen. Reproduction in Domestic Animals 43 (Suppl 2) 338-346. (doi:10.1111/j.1439-0531.2008.01182.x).
116. Rath D, Johnson LA & Welch GR 1993 In vitro culture of porcine embryos: development to blastocysts after in vitro fertilization (IVF) with flow cytometrically sorted and unsorted semen. Theriogenology 39 293 (Abstr). (doi:10.1016/0093-691X(93)90148-X).
117. Rath D, Johnson LA, Welch GR & Niemann H 1994 Successful gamete intrafallopian transfer (GIFT) in the porcine. Theriogenology 41 1173-1179. (doi:10.1016/S0093-691X(05)80039-1).
118. Rath D, Johnson LA, Dobrinsky JR, Welch GR & Niemann H 1997 Production of piglets preselected for sex following in vitro fertilization with X and Y chromosome-bearing spermatozoa sorted by flow cytometry. Theriogenology 47 795-800. (doi:10.1016/S0093-691X(97) 00035-6).
119. Rath D, Long CR, Dobrinsky JR, Welch GR, Schreier LL & Johnson LA 1999 In vitro production of sexed embryos for gender preselection: high-speed sorting of X-chromosome-bearing sperm to produce pigs after embryo transfer. Journal of Animal Science 77 3346-3352.
120. Rath D, Ruiz S & Sieg B 2003 Birth of female piglets following intrauterine insemination of a sow using flow cytometrically sexed boar semen. Veterinary Record 152 400-401. (doi:10.1136/vr.152.13.400).
121. Rath D, Bathgate R, Rodriguez-Martinez H, Roca J, Strzezek J & Waberski D 2009a Recent advances in boar semen cryopreservation. Society of Reproduction and Fertility Supplement 66 51-66.
122. Rath D, Moench-Tegeder G, Taylor U & Johnson LA 2009b Improved quality of sex-sorted sperm: a prerequisite for wider commercial application. Theriogenology 71 22-29. (doi:10.1016/j.theriogenology. 2008.09.027).
123. Rens W, Welch GR & Johnson LA 1998 A novel nozzle for more efficient sperm orientation to improve sorting efficiency of X and Y chromosomebearing sperm. Cytometry 33 476-481. (doi:10.1002/(SICI)1097- 0320(19981201)33:4!476:: AID-CYTO12O3.0.CO;2-B).
124. Rigby SL, Brinsko SP, CochranM, Blanchard TL, Love CC & Varner DD 2001 Advances in cooled semen technologies: seminal plasma and semen extender. Animal Reproduction Science 68 171-180. (doi:10.1016/S0378- 4320(01)00154-3).
125. Salmaso S, Caliceti P, Amendola V, Meneghetti M, Magnusson JP, Pasparakis G & Alexander C 2009 Cell up-take control of gold nanoparticles functionalized with a thermoresponsive polymer. Journal of Materials Chemistry 19 1608-1615. (doi:10.1039/b816603j).
126. Sauer H, Bekhite MM, Hescheler J &Wartenberg M 2005 Redox control of angiogenic factors and CD31-positive vessel-like structures in mouse embryonic stem cells after direct current electrical field stimulation. Experimental Cell Research 304 380-390. (doi:10.1016/j.yexcr.2004. 11.026).
127. Schenk JL & Seidel GE Jr 2007 Pregnancy rates in cattle with cryopreserved sexed spermatozoa: effects of laser intensity, staining conditions and catalase. Society of Reproduction and Fertility Supplement 64 165-177.
128. Schenk JL, Suh TK & Seidel GE Jr 2006 Embryo production from superovulated cattle following insemination of sexed sperm. Theriogenology 65 299-307. (doi:10.1016/j.theriogenology.2005.04.026).
129. Schenk JL, Cran DG, Everett RW & Seidel GE Jr 2009 Pregnancy rates in heifers and cows with cryopreserved sexed sperm: effects of sperm numbers per inseminate, sorting pressure and sperm storage before sorting. Theriogenology 71 717-728. (doi:10.1016/j.theriogenology. 2008.08.016).
130. Schuberth HJ, Taylor U, Zerbe H, Waberski D, Hunter R & Rath D 2008 Immunological responses to semen in the female genital tract. Theriogenology 70 1174-1181. (doi:10.1016/j.theriogenology.2008. 07.020).
131. Schrand AM, Rahman MF, Hussain SM, Schlager JJ, Smith DA & Syed AF 2010 Metal-based nanoparticles and their toxicity assessment. Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology 2 544-568. (doi:10.1002/wnan.103).
132. Schwarz-Finsterle J, Stein S, Grossmann C, Schmitt E, Trakhtenbrot L, Rechavi G, Amariglio N, Cremer C & Hausmann M 2007 Comparison of triple helical COMBO-FISH and standard FISH by means of quantitative microscopic image analysis of abl/bcr positions in cell nuclei. Journal of Biochemical and Biophysical Methods 70 397-406. (doi:10.1016/j. jbbm.2006.09.004).
133. Seidel GE Jr, Schenk JL, Herickhoff LA, Doyle SP, Brink Z, Green RD & Cran DG 1999 Insemination of heifers with sexed sperm. Theriogenology 52 1407-1420. (doi:10.1016/S0093-691X(99)00226-5).
134. Seidman MM & Glazer PM 2003 The potential for gene repair via triple helix formation. Journal of Clinical Investigation 112 487-494.
135. Sharpe JC & Evans KM 2009 Advances in flow cytometry for sperm sexing. Theriogenology 71 4-10. (doi:10.1016/j.theriogenology.2008.09.021).
136. Shi XG, Wang SH, Meshinchi S, Van Antwerp ME, Bi XD, Lee IH & Baker JR 2007 Dendrimer-entrapped gold nanoparticles as a platform for cancercell targeting and Imaging. Small 3 1245-1252. (doi:10.1002/smll. 200700054).
137. Shi TY, Chen G, Huang X, Yuan Y, Wu X, Wu B, Li Z, Shun F, Chen H & Shi H 2012 Effects of reactive oxygen species from activated leucocytes on human sperm motility, viability and morphology. Andrologia 44 (Suppl 1) 696-703.
138. Shukla R, Bansal V, Chaudhary M, Basu A, Bhonde RR & Sastry M 2000 Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview. Langmuir: The ACS Journal of Surfaces and Colloids 21 10644-10654. (doi:10.1021/la0513712).
139. Simpson AM, Swan MA &White IG 1986 Action of phosphatidylcholine in protecting ram sperm from cold shock. Gamete Research 15 43-56. (doi:10.1002/mrd.1120150106).
140. Simpson AM, Swan MA &White IG 1987 Calcium uptake, respiration, and ultrastructure of sperm exposed to ionophore A23187. Archives of Andrology 19 5-18. (doi:10.3109/01485018708986795).
141. Spinaci M, Volpe S, Bernardini C, de Ambrogi M, Tamanini C, Seren E & Galeati G 2006 Sperm sorting procedure induces a redistribution of Hsp70 but not Hsp60 and Hsp90 in boar spermatozoa. Journal of Andrology 27 899-907. (doi:10.2164/jandrol.106.001008).
142. Spinaci M, Vallorani C, Bucci D, Bernardini C, Tamanini C, Seren E & Galeati G 2010 Effect of liquid storage on sorted boar spermatozoa. Theriogenology 74 741-748. (doi:10.1016/j.theriogenology. 2010.03.027).
143. Stap J, Hoebe RA, Merton JS, Haring RM, Bakker PJM & Aten JA 1998 Improving the resolution of cryopreserved X- and Y-sperm during DNA flow cytometric analysis with the addition of Percoll to quench the fluorescence of dead sperm. Journal of Animal Science 76 1896-1902.
144. Stewart KM, Horton KL & Kelley SO 2008 Cell-penetrating peptides as delivery vehicles for biology and medicine. Organic & Biomolecular Chemistry 6 2242-2255. (doi:10.1039/b719950c).
145. Storey BT 2008 Mammalian sperm metabolism: oxygen and sugar, friend and foe. International Journal of Developmental Biology 52 427-437. (doi:10.1387/ijdb.072522bs).
146. Stovel RT, Sweet RG & Herzenberg LA 1978 A means for orienting flat cells in flow systems. Biophysical Journal 23 1-5. (doi:10.1016/S0006-3495 (78)85427-7).
147. Sun Z, Niu R, Su K, Wang B, Wang J, Zhang J & Wang J 2010 Effects of sodium fluoride on hyperactivation and Ca2C signalling pathways in sperm from mice: an in vivo study. Archives of Toxicology 84 351-361. (doi:10.1007/s00204- 009-0508-x).
148. Tomkins PT & Houghton JA 1988 The rapid induction of the acrosome reaction of human spermatozoa by electropermeabilization. Fertility and Sterility 50 329-336.
149. Taylor U, Rath D, Zerbe H & Schuberth HJ 2008 Interaction of intact porcine spermatozoa with epithelial cells and neutrophilic granulocytes during uterine passage. Reproduction in Domestic Animals 43 166-175. (doi:10.1111/j.1439-0531.2007.00872.x).
150. Taylor U, Schuberth HJ, Rath D, Michelmann HW, Sauter-Louis C & Zerbe H 2009a Influence of inseminate components on porcine leucocyte migration in vitro and in vivo after pre- and post-ovulatory insemination. Reproduction in Domestic Animals 44 180-188. (doi:10.1111/j.1439-0531.2007.01015.x).
151. Taylor U, Zerbe H, Seyfert HM, Rath D, Baulain U, Langner KF & Schuberth HJ 2009b Porcine spermatozoa inhibit post-breeding cytokine induction in uterine epithelial cells in vivo. Animal Reproduction Science 115 279-289. (doi:10.1016/j.anireprosci.2008.11.019).
152. Taylor U, Zerbe H, Seyfert HM, Rath D & Schuberth HJ 2009c Binding of porcine spermatozoa to uterine epithelial cells modulates the female immune response andmight indicate the formation of a pre-oviductal sperm reservoir. Society of Reproduction and Fertility Supplement 66 83-84.
153. Taylor U, Klein S, Petersen S, Kues W, Barcikowski S & Rath D 2010 Nonendosomal cellular uptake of ligand-free, positively charged gold nanoparticles. Cytometry. Part A 77 439-446. (doi:10.1002/cyto. a.20846).
154. Taylor U, Barchanski A, Garrels W, Klein S, Kues W, Barcikowski S & Rath D 2012 Toxicity of gold nanoparticles on somatic and reproductive cells. Advances in Experimental Medicine and Biology 733 125-133. (doi:10.1007/978-94- 007-2555-3-12).
155. Tkachenko AG, Xie H, Liu YL, Coleman D, Ryan J, Glomm WR, Shipton MK, Franzen S & Feldheim DL 2004 Cellular trajectories of peptide-modified gold particle complexes: comparison of nuclear localization signals and peptide transduction domains. Bioconjugate Chemistry 15 482-490. (doi:10.1021/bc034189q) .
156. Underwood SL, Bathgate R, Maxwell WMC & Evans G 2009a Development of procedures for sex-sorting frozen-thawed bovine spermatozoa. Reproduction in Domestic Animals 44 460-466. (doi:10.1111/j.1439- 0531.2008.01127.x).
157. Underwood SL, Bathgate R, Maxwell WMC & Evans G 2009b In vitro characteristics of frozen-thawed, sex-sorted bull sperm after refreezing or incubation at 15 or 37 8C. Theriogenology 72 1001-1008. (doi:10.1016/j. theriogenology.2009.06.023).
158. Underwood SL, Bathgate R, Ebsworth M, Maxwell WMC & Evans G 2010a Pregnancy loss in heifers after artificial insemination with frozen-thawed, sex-sorted, re-frozen-thawed dairy bull sperm. Animal Reproduction Science 118 7-12. (doi:10.1016/j.anireprosci.2009.06.004).
159. Underwood SL, Bathgate R, Maxwell WMC & Evans G 2010b Birth of offspring after artificial insemination of heifers with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm. Animal Reproduction Science 118 171-175. (doi:10.1016/j.anireprosci.2009.08.007).
160. Underwood SL, Bathgate R, Pereira DC, Castro A, Thomson PC, Maxwell WMC & Evans G 2010c Embryo production after in vitro fertilization with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm. Theriogenology 73 97-102. (doi:10.1016/j.theriogenology. 2009.08.005).
161. Van Dilla MA, Gledhill BL, Lake S, Dean PN, Gray JW, Kachel V, Barlogie B & Gohde W 1977 Measurement of mammalian sperm deoxyribonucleic acid by flow cytometry. Problems and approaches. Journal of Histochemistry and Cytochemistry 25 763-773. (doi:10.1177/25.7.70455).
162. Vazquez JM, Martinez EA, Parrilla I, Roca J, GilMA & Vazquez JL 2003 Birth of piglets after deep intrauterine insemination with flow cytometrically sorted boar spermatozoa. Theriogenology 59 1605-1614. (doi:10.1016/S0093- 691X(02)01198-6).
163. Vazquez JM, Martinez EA, Roca J, Gil MA, Parrilla I, Cuello C, Carvajal G, Lucas X & Vazquez JL 2005 Improving the efficiency of sperm technologies in pigs: the value of deep intrauterine insemination. Theriogenology 63 536-547. (doi:10.1016/j.theriogenology. 2004.09.043).
164. Vazquez JM, Roca J, Gil MA, Cuello C, Parrilla I, Caballero I, Vazquez JL & Martinez EA 2008 Low-dose insemination in pigs: problems and possibilities. Reproduction in Domestic Animals 43 (Suppl 2) 347-354. (doi:10.1111/j.1439-0531.2008.01183.x).
165. Verma A, Uzun O, Hu YH, Hu Y, Han HS, Watson N, Chen SL, Irvine DJ & Stellacci F 2008 Surface structure-regulated cell-membrane penetration by monolayer protected nanoparticles. Nature Materials 7 588-595. (doi:10.1038/nmat2202).
166. Weiss L & Zeigel R 1971 Cell surface negativity and binding of positively charged particles. Journal of Cellular Physiology 77 179-186. (doi:10.1002/jcp.1040770208).
167. Xu J, Guo Z, Su L, Nedambale TL, Zhang J, Schenk J, Moreno JF, Dinnyés A, Ji W, Tian XC et al. 2006 Developmental potential of vitrified Holstein cattle embryos fertilized in vitro with sex-sorted sperm. Journal of Dairy Science 89 2510-2518. (doi:10.3168/jds. S0022-0302(06)72326-8).
168. Zakrzewska H & Udala J 2006 In vitro influence of sodium fluoride on adenosine triphosphate (ATP) content in ram semen. Annales Academiae Medicae Stetinensis 52 (Suppl 1) 109-111.
169. Zakrzewska H, Udala J & Blaszczykb B 2002 In vitro influence of sodium fluoride on ram semen quality and enzyme activities. Fluoride 35 153-160. (doi:10.2147/IJN.S8428).
170. Zhang XD, Wu HY, Wu D, Wang YY, Chang JH, Zhai ZB, Meng AM, Liu PX, Zhang LA & Fan FY 2010 Toxicologic effects of gold nanoparticles in vivo by different administration routes. International Journal of Nanomedicine 5 771-781. (doi:10.2147/IJN.S8428).