Improved quality of sex-sorted sperm: A prerequisite for wider commercial application

Theriogenology

Volumn 71, Issue 1, 2009, Pages 22-29

  Rath, D ^a   Moench Tegeder, G ^a   Taylor, U ^a   Johnson, L A ^b  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^b Mount Airy   (United States)

Author keywords

Improved sorting technology; Minimum standards; Sperm quality; Sperm sorting

Indexed keywords

ANIMAL; ANIMAL DISEASE; ARTICLE; ARTIFICIAL INSEMINATION; CATTLE; FEMALE; FERTILITY; GENETIC ENGINEERING; MALE; METHODOLOGY; PHYSIOLOGY; QUALITY CONTROL; SPERM PRESERVATION; SPERMATOZOON; SWINE;

ANIMALS; CATTLE; FEMALE; FERTILITY; INSEMINATION, ARTIFICIAL; MALE; QUALITY CONTROL; SEMEN PRESERVATION; SEX PRESELECTION; SPERMATOZOA; SWINE;

EID: 56549106159     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2008.09.027     Document Type: Article

References (65)

1. Foote R.H., and Miller P. What might sex ratio control mean in the animal world?. In: Kiddy C.A., and Hafs H.D. (Eds). Sex ratio at birth prospects for control. (1971), American Society of Animal Science, Savoy, IL 1-9
2. Johnson L.A., Flook J.P., and Hawk H.W. Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting. Biol Reprod 41 (1989) 199-203
3. Ettma J.F., Hoag D.L., and Seidel Jr. G.E. Economic opportunities for sexed semen on commercial dairies. Western Dairy News 7 (2007) 67-68
4. De Vries A., Overton M., Fetrow J., Leslie K., Eicker S., and Rogers G. Exploring the impact of sexed semen on the structure of the dairy industry. J Dairy Sci 91 (2008) 847-856
5. Rath D., and Johnson L.A. Application and commercialization of flow cytometrically sex-sorted semen. Reprod Dom Anim 43 Suppl. 2 (2008) 338-346
6. de Graaf S.P., Evans G., Maxwell W.M., Cran D.G., and O'Brien J.K. Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa. Theriogenology 67 (2007) 391-398
7. Johnson L.A., Welch G.R., and Rens W. The Beltsville sperm sexing technology: high speed sperm sorting gives improved sperm output for in vitro fertilization and AI. J Anim Sci 77 Suppl 2 (1999) 213-220
8. Rens W., Welch G.R., and Johnson L.A. A novel nozzle for more efficient sperm orientation to improve sorting efficiency of X and Y chromosome-bearing sperm. Cytometry 33 (1998) 476-481
9. Seidel Jr. G.E., and Garner D.L. Current status of sexing mammalian spermatozoa. Reproduction 124 (2002) 733-743
10. Maxwell W.M.C., and Johnson L.A. Physiology of spermatozoa at high dilution rates: the influence of seminal plasma. Theriogenology 52 (1999) 1353-1362
11. Rath D., Ruiz S., and Sieg B. Birth of female piglets following intrauterine insemination of a sow using flow cytometrically sexed boar semen. Vet Rec 152 (2003) 400-401
12. Grossfeld R., Klinc P., Sieg B., and Rath D. Production of piglets with sexed semen employing a non-surgical insemination technique. Theriogenology 63 (2005) 2269-2277
13. Johnson L.A., Flook J.P., and Look M.V. Flow cytometry of X and Y chromosome-bearing sperm for DNA using an improved preparation method and staining with Hoechst 33342. Gamete Res 17 (1987) 203-212
14. Johnson L.A. Sex preselection in swine: altered sex ratios in offspring following surgical insemination of flow sorted X- and Y-bearing sperm. Reprod Dom Anim 26 (1991) 309-314
15. Johnson L.A. Sex preselection by flow cytometric separation of X- and Y-chromosome bearing sperm based on DNA difference: a review. Reprod Fertil Dev 7 (1995) 893-903
16. Cran D.G., Johnson L.A., Miller N.G.A., Cochrane D., and Polge C.E. Production of bovine calves following separation of X- and Y-bearing sperm and in vitro fertilization. Vet Rec 132 (1993) 40-41
17. Seidel G.E. Sexing mammalian sperm and embryos-state of the art. J Reprod Fertil Suppl. 54 (1999) 477-487
18. Hollinshead F.K., O'Brien J.K., Maxwell W.M.C., and Evans G. Production of lambs of predetermined sex after the insemination of ewes with low numbers of frozen-thawed sorted X- or Y-chromosome bearing spermatozoa. Reprod Fertil Dev 14 (2002) 503-508
19. Seidel Jr. G.E., Allen C.H., Johnson L.A., Holland M.D., Brink Z., Welch G.R., et al. Uterine horn insemination of heifers with very low numbers of nonfrozen and sexed spermatozoa. Theriogenology 48 (1997) 1255-1265
20. Maxwell W.M.C., Hollinshead F.K., Rath D., O'Brien J.K., and Evans G. Effect of dose of sperm processed for sex-sorting and cryopreservation on fertility in ewes. Theriogenology 59 511 (2003) [Abstr.]
21. Johnson L.A., and Welch G.R. Sex preselection: high-speed flow cytometric sorting of X- and Y-sperm for maximum efficiency. Theriogenology 52 (1999) 1134-1323
22. Spinaci M., De Ambrogi M., Volpe S., Galeati G., Tamanini C., and Seren E. Effect of staining and sorting on boar sperm membrane integrity, mitochondrial activity and in vitro blastocyst development. Theriogenology 64 (2005) 191-201
23. Merton J.S., Haring R.M., Stap J., Hoebe R.A., and Aten J.A. Effect of flow cytometrically sorted frozen/thawed semen on success rate of in vitro bovine embryo production. Theriogenology 47 (1997) 295 [Abstract]
24. Zhang M., Lu K.H., and Seidel G.E. Development of bovine embryos after in vitro fertilization of oocytes with flow cytometrically sorted, stained and unsorted sperm from different bulls. Theriogenology 60 (2003) 1657-1663
25. Johnson L.A., Cran D.G., Welch G.R., and Polge C. Gender preselection in mammals. In: Miller R.H., Pursel V.G., and Norman H.D. (Eds). Beltsville symbosium XX: biotechnology's role in the genetic improvement of farm animals (1996), Amer Soc Anim Sci, Savoy, IL p.151-164
26. Guthrie H.D., Johnson L.A., Garrett W.M., Welch G.R., and Dobrinsky J.R. Flow cytometric sperm sorting: effects of varying laser power on embryo development in swine. Mol Reprod Dev 61 (2002) 87-92
27. Catt S.L., Sakkas D., Bizzaro D., Bianchi P.G., Maxwell W.M.C., and Evans G. Hoechst staining and exposure to UV laser during flow cytometric sorting does not affect the frequency of detected endogenous DNA nicks in abnormal and normal human sperm. Mol Hum Reprod 3 (1997) 821-825
28. Parrilla I., Vazquez J.M., Cuello C., Gil M.A., Roca J., Di Berardino D., et al. Hoechst 33342 stain and UV laser exposure do not induce genotoxic effects in flow-sorted boar spermatozoa. Reproduction 128 (2004) 615-621
29. Boe-Hansen G.B., Morris I.D., Annette B., Ersbølla K., Greve T., and Christensen P. DNA integrity in sexed bull sperm assessed by neutral Comet assay and sperm chromatin structure assay. Theriogenology 63 (2005) 1789-1802
30. De Ambrogi M., Spinaci M., Galeati G., and Tamanini C. Viability and DNA fragmentation in differently sorted boar spermatozoa. Theriogenology 66 (2006) 1994-2000
31. Maxwell W.M.C., Welch G.R., and Johnson L.A. Viability and membrane integrity of spermatozoa after dilution and flow cytometric sorting in the presence or absence of seminal plasma. Reprod Fertil Dev 8 (1997) 1165-1178
32. Maxwell W.M.C., Long C.R., Johnson L.A., Dobrinsky J.R., and Welch G.R. The relationship between membrane status and fertility of boar spermatozoa after flow cytometric sorting in the presence or absence of seminal plasma. Reprod Fertil Dev 10 (1998) 433-440
33. Maxwell W.M.C., and Johnson L.A. Chlortetracycline analysis of boar spermatozoa after incubation, flow cytometric sorting, cooling or cryopreservation. Mol Reprod Dev 46 (1997) 408-418
34. Watson P.F. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod Fertil Dev 74 (1995) 871-891
35. Rath D., Sieg B., Leigh J., Klinc P., Besseling M., Krüger C., et al. Current perspectives of sperm sorting in domestic farm animals. Proceedings of the 19th Mtg Assoc Européenne de Transfert Embryonnaire (2003) 125-128
36. Centurion F., Vazquez J.M., Calvete J.J., Roca J., Sanz L., Parrilla I., et al. Influence of porcine sperm adhesions on the susceptibility of boar spermatozoa to high dilution. Biol Reprod 69 (2003) 640-646
37. Spinnaci M., Volpe S., Bernardini C., de Ambrogi M., Tamanini C., Seren E., et al. Sperm sorting procedure induces a redistribution of Hsp70 but not Hsp60 and Hsp90 in boar spermatozoa. J Androl 27 (2006) 899-907
38. Garcia E.M., Vazquez J.M., Parrilla I., Calvete J.J., Sanz L., Caballero I., et al. Improving the fertilizing ability of sex sorted boar spermatozoa. Theriogenology 68 (2007) 771-778
39. Campos-Chillon L.F., and de la Torre J.F. Effect on concentration of sexed bovine sperm sorted at 40 and 50 psi on developmental capacity of in vitro produced embryos. Theriogenology 59 (2003) 506 [Abstr.]
40. Suh T.K., Schenk J.L., and Seidel Jr. G.E. High pressure flow cytometric sorting damages sperm. Theriogenology 64 (2005) 1035-1048
41. Klinc P., and Rath D. Reduction of oxidative stress in bovine spermatozoa during flow cytometric sorting. Reprod Dom Anim 42 (2007) 63-67
42. Cran D.G., Cochrane D.J., Johnson D.J., Wei H., Lu K.H., and Polge C. Separation of X- and Y-chromosome bearing bovine sperm by flow cytometry for use in IVF. Theriogenology 41 (1994) 183 [Abstr.]
43. Lu K.H., Cran D.G., and Seidel G.E. In vitro fertilization with flow-cytometrically sorted bovine sperm. Theriogenology 52 (1999) 1393-1405
44. McNutt T.L., and Johnson L.A. Flow cytometric sorting of sperm: influence on fertilization and embryo/fetal development in the rabbit. Mol Reprod Dev 43 (1996) 261-267
45. Morton K.M., Herrmann D., Sieg B., Struckmann C., Maxwell W.M., Rath D., et al. Altered mRNA expression patterns in bovine blastocysts after fertilisation in vitro using flow-cytometrically sex-sorted sperm. Mol Reprod Dev 74 (2007) 931-940
46. Bermejo-Alvarez P., Rizos D., Rath D., Lonergan P., and Gutierrez-Adan A. Epigenetic differences between male and female bovine blastocysts produced in vitro. Physiol Genom 32 (2008) 264-272
47. Beyhan Z., Johnson L.A., and First N.L. Sexual dimorphism in IVM-IVF bovine embryos produced from X and Y chromosome-bearing spermatozoa sorted by high speed flow cytometry. Theriogenology 52 (1999) 35-48
48. Cran D.G., and Johnson L.A. The prediction of embryonic sex using flow cytometrically separated X and Y spermatozoa. Hum Reprod Update 2 (1996) 355-363
49. Morton K.M., Catt S.L., Hollinshead F.K., Maxwell W.M.C., and Evans G. The effect of gamete co-incubation time during in vitro fertilization with frozen-thawed unsorted and sex-sorted ram spermatozoa on the development of in vitro matured adult and prepubertal ewe oocytes. Theriogenology 64 (2005) 363-377
50. Palma G.A., Olivier N.S., Neumüller C., and Sinowatz F. Effects of sex-sorted spermatozoa on the efficiency of in vitro fertilization and ultrastructure of in vitro produced bovine blastocysts. Anat Histol Embryol 37 (2008) 67-73
51. Rath D., Johnson L.A., Dobrinsky J.R., Welch G.R., and Niemann H. Production of piglets preselected for sex following in vitro fertilization with X and Y chromosome-bearing spermatozoa sorted by flow cytometry. Theriogenology 47 (1997) 795-800
52. Rath D., Long C.R., Dobrinsky J.R., Welch G.R., Schreier L.L., and Johnson L.A. In vitro production of sexed embryos for gender preselection: high-speed sorting of X-chromosome-bearing sperm to produce pigs after embryo transfer. J Anim Sci 77 (1999) 3346-3352
53. Probst S., and Rath D. Production of piglets using intracytoplasmic sperm injection (ICSI) with flow cytometrically sorted boar semen and artificially activated oocytes. Theriogenology 59 (2003) 961-973
54. Den Daas J.H., De Jong G., Lansbergen L.M., and Van Wagtendonk-De Leeuw A.M. The relationship between the number of spermatozoa inseminated and the reproductive efficiency of individual dairy bulls. J Dairy Sci 81 (1998) 1714-1723
55. Andersson M., Taponen J., Koskinen E., and Dahlbom M. Effect of insemination with doses of 2 or 15 million frozen-thawed spermatozoa and semen deposition site on pregnancy rate in dairy cows. Theriogenology 61 (2004) 1583-1588
56. Ballester J., Johannisson A., Saravia F., Håård M., Gustafsson H., Bajramovic D., et al. Post-thaw viability of bull AI-doses with low-sperm numbers. Theriogenology 68 (2007) 934-943
57. Andersson M., Taponen J., Kommeri M., and Dahlbom M. Pregnancy rates in lactating Holstein-Friesian cows after artificial insemination with sexed sperm. Reprod Dom Anim 41 (2006) 95-97
58. DeJarnette J.M., Nebel R.L., Marshall C.E., Moreno J.F., McCleary C.R., and Lenz R.W. Effect of sex-sorted sperm dosage on conception rates in Holstein heifers and lactating cows. J Dairy Sci 91 (2008) 1778-1785
59. Klinc P., Frese D., Osmers H., and Rath D. Insemination with sex sorted fresh bovine spermatozoa processed in the presence of antioxidative substances. Reprod Dom Anim 42 (2007) 58-62
60. Welch G.R., and Johnson L.A. Sex preselection: laboratory validation of the sperm sex ratio of flow sorted X and Y-sperm by sort reanalysis for DNA. Theriogenology 52 (1999) 1343-1352
61. Kawarasaki T., Welch G.R., Long C.R., Yoshida M., and Johnson L.A. Verification of flow cytometrically sorted X- and Y-bearing porcine spermatozoa and reanalysis of spermatozoa for DNA content using the fluorescence in situ hybridization (FISH) technique. Theriogenology 50 (1998) 625-635
62. Rens W., Yang F., Welch G., Revell S., O'Brien P.C., Solanky N., et al. An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures. Reproduction 121 (2001) 541-546
63. Welch G.R., Walsbieser G.C., Wall R.J., and Johnson L.A. Flow cytometric sperm sorting and PCR to confirm separation of X- and Y-chromosome bearing bovine sperm. Anim Biotechnol 6 (1995) 131-139
64. Evenson D.P., Jost L.K., Marshall D., Zinaman M.J., Clegg E., Purvis K., et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod 14 (1999) 1039-1049
65. Garner D.L., Johnson L.A., Yue S.T., Roth B.L., and Haugland R.P. Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide. J Androl 15 (1994) 620-629