Positively charged DNA-binding proteins cause apparent cell membrane translocation

Biochemical and Biophysical Research Communications

Volumn 291, Issue 2, 2002, Pages 367-371

  Lundberg, Mathias ^a   Johansson, Magnus ^b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Gene therapy; Immunocytochemistry; Membrane translocation; Protein delivery; Protein therapeutics; Protein transduction domain

Indexed keywords

DNA BINDING PROTEIN; HERPES SIMPLEX VIRUS PROTEIN VP22; HISTONE H1; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ANIMAL CELL; ARTICLE; ARTIFACT; CELL MEMBRANE PERMEABILITY; CELL MEMBRANE TRANSPORT; CELL NUCLEUS; CONTROLLED STUDY; FEMALE; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; SURFACE CHARGE;

HUMAN IMMUNODEFICIENCY VIRUS; SIMPLEXVIRUS;

EID: 0036289650     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2002.6450     Document Type: Article

References (24)

1. Protein transduction: Unrestricted delivery into all cells?
2. Cellular uptake of the tat protein from human immunodeficiency virus
3. Tat-mediated delivery of heterologous proteins into cells
4. Arginine-rich peptides. An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery
5. A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus
6. In vivo protein transduction: Delivery of a biologically active protein into the mouse
7. Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration
8. Synthetic protein transduction domains: Enhanced transduction potential in vitro and in vivo
9. Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells
10. Killing HIV-infected cells by transduction with an HIV protease-activated caspase-3 protein
11. The third helix of the Antennapedia homeodomain translocates through biological membranes
12. Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independent
13. A 16-mer peptide (RQIKIWFQNRRMKWKK) from Antennapedia preferentially targets the class I pathway
14. Intercellular trafficking and protein delivery by a herpesvirus structural protein
15. Evaluation of VP22 spread in tissue culture
16. Intercellular spread of GFP-VP22
17. Intercellular trafficking of VP22-GFP fusion proteins
18. Quantification of VP22-GFP spread by direct fluorescence in 15 commonly used cell lines
19. Conformational lability of herpesvirus protein VP22
20. Intercellular delivery of thymidine kinase prodrug activating enzyme by the herpes simplex virus protein, VP22
21. Intercellular delivery of functional p53 by the herpesvirus protein VP22
22. Is VP22 nuclear homing an artifact?
23. The HIV Tat protein transduction domain improves the biodistribution of beta-glucuronidase expressed from recombinant viral vectors
24. Ability of the Tat basic domain and VP22 to mediate cell binding, but not membrane translocation of the diphtheria toxin A-fragment