In vitro characteristics of frozen-thawed, sex-sorted bull sperm after refreezing or incubation at 15 or 37 °C

Theriogenology

Volumn 72, Issue 7, 2009, Pages 1001-1008

  Underwood, S L ^a   Bathgate, R ^a   Maxwell, W M C ^a   Evans, G ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Cryopreservation; Semen analysis; Sex preselection; Sperm sexing

Indexed keywords

ACROSOME; ANIMAL; ANIMAL DISEASE; ARTICLE; CATTLE; CELL SEPARATION; COMPARATIVE STUDY; CRYOPRESERVATION; MALE; METHODOLOGY; MITOCHONDRION; PHYSIOLOGY; SEX DETERMINATION; SPERM PRESERVATION; SPERMATOZOON; SPERMATOZOON MOTILITY; TEMPERATURE; ULTRASTRUCTURE;

ACROSOME; ANIMALS; CATTLE; CELL SEPARATION; CRYOPRESERVATION; MALE; MITOCHONDRIA; SEMEN PRESERVATION; SEX DETERMINATION ANALYSIS; SPERM MOTILITY; SPERMATOZOA; TEMPERATURE;

EID: 70149103083     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2009.06.023     Document Type: Article

References (22)

1. Stap J., Hoebe R.A., Merton J.S., Haring R.M., Bakker P.J.M., and Aten J.A. Improving the resolution of cryopreserved X- and Y-sperm during DNA flow cytometric analysis with the addition of Percoll to quench the fluorescence of dead sperm. J Anim Sci 76 (1998) 1896-1902
2. Lu K.H., Cran D.G., and Seidel Jr. G.E. In vitro fertilization with flow-cytometrically-sorted bovine sperm. Theriogenology 52 (1999) 1393-1405
3. Hollinshead F.K., Evans G., Evans K.M., Catt S.L., Maxwell W.M.C., and O'Brien J.K. Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen-thawed sex- and re-frozen-thawed ram spermatozoa. Reproduction 127 (2004) 557-568
4. de Graaf S.P., Evans G., Maxwell W.M.C., Cran D.G., and O'Brien J.K. Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa. Theriogenology 67 (2007) 391-398
5. Hollinshead F.K., O'Brien J.K., Maxwell W.M.C., and Evans G. Assessment of in vitro sperm characteristics after flow cytometric sorting of frozen-thawed bull spermatozoa. Theriogenology 62 (2004) 958-968
6. de Graaf S.P., Evans G., Maxwell W.M.C., and O'Brien J.K. In vitro characteristics of fresh and frozen-thawed ram spermatozoa after sex sorting and re-freezing. Reprod Fertil Dev 18 (2006) 867-874
7. Puglisi R., Vanni R., Galli A., Balduzzi D., Parati K., Bongioni G., et al. In vitro fertilisation with frozen-thawed bovine sperm sexed by flow cytometry and validated for accuracy by real-time PCR. Reproduction 132 (2006) 519-526
8. Underwood S.L., Bathgate R., Maxwell W.M.C., and Evans G. Development of procedures for sex-sorting frozen-thawed bovine spermatozoa. Reprod Domest Anim 44 (2009) 460-466
9. Underwood SL, Bathgate R, Ebsworth M, Maxwell WMC, Evans G., Pregnancy loss in heifers after artificial insemination with frozen-thawed, sex-sorted, re-frozen-thawed dairy bull sperm. Anim Reprod Sci 2009, doi:10.1016/j.anireprosci.2009.06.004, in press.
10. Evans G., and Maxwell W.M.C. Salamon's Artificial Insemination of Sheep and Goats (1987), Butterworths
11. Klinc P., and Rath D. Reduction of oxidative stress in bovine spermatozoa during flow cytometric sorting. Reprod Domest Anim 42 (2007) 63-67
12. Schenk J.L., Suh T.K., and Cran D.G. Seidel GE Jr. Cryopreservation of flow-sorted bovine spermatozoa. Theriogenology 52 (1999) 1375-1391
13. Holt W.V., Morris G.J., Coulson G., and North R.D. Direct observation of cold-shock effect in ram spermatozoa with the use of a programmable cryomicroscope. J Exp Zool 246 (1998) 305-314
14. Watson P.F. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci 60-61 (2000) 481-492
15. Celeghini E.C.C., de Arruda R.P., de Andrade A.F.C., Nascimento J., Raphael C.F., and Rodrigues P.H.M. Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin. Anim Reprod Sci 104 (2008) 119-131
16. Watson P.F. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod Fertil Dev 7 (1995) 871-891
17. Hammerstedt R.H., Graham J.K., and Nolan J.P. Cryopreservation of mammalian sperm: what we ask them to survive. J Androl 11 (1990) 73-88
18. Parks J.E., and Graham J.K. Effects of cryopreservation procedures on sperm membranes. Theriogenology 38 (1992) 209-222
19. Johnson L.A., and Welch G.R. Sex preselection: high-speed flow cytometric sorting of X and Y sperm for maximum efficiency. Theriogenology 52 (1999) 1323-1341
20. ® density gradient centrifugation. Reprod Domest Anim 42 (2007) 489-494
21. Cseh S., Polichronopoulos T., and Solti L. Prediction of bull fertility by computer-assisted semen analysis. Reprod Fert Devel 16 (2004) 128-129
22. Gillan L., Kroetsch T., Maxwell W.M.C., and Evans G. Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls. Anim Reprod Sci 103 (2008) 201-214