The detection of viable vegetative cells of Bacillus sporothermodurans using propidium monoazide with semi-nested PCR

Food Microbiology

Volumn 34, Issue 1, 2013, Pages 196-201

  Cattani, F ^a   Ferreira, C A S ^a   Oliveira, S D ^a  

^a PONTIFICAL CATHOLIC UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

Bacillus sporothermodurans; Detection; Milk; Propidium monoazide; Semi nested PCR; Viable cells

Indexed keywords

AZIDE; DRUG DERIVATIVE; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE;

ANIMAL; ARTICLE; BACILLUS; BACTERIAL SPORE; CATTLE; CHEMISTRY; EVALUATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MILK; POLYMERASE CHAIN REACTION; STAINING;

ANIMALS; AZIDES; BACILLUS; CATTLE; MILK; POLYMERASE CHAIN REACTION; PROPIDIUM; SPORES, BACTERIAL; STAINING AND LABELING;

BACILLUS SPOROTHERMODURANS; BACTERIA (MICROORGANISMS);

EID: 84872811981     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2012.12.007     Document Type: Article

References (36)

1. Anonymous Council directive 92/46/EEC of 16 June 1992 laying down the health rules for the production and placing on the market of raw milk, heat-treated milk and milk-based products. Official Journal of the European Communities 4.9.1992, L268:1-32. (accessed 16.07.12). http://eurex.europa.eu/LexUriServ/LexUriServ.do%3furi%3dCELEX:31992L0046:EN:HTML.
2. Arenas R., Gonzales L., Bernardo A., Fresno J.M., Tornadijo M.E. Microbiological and physico-chemical changes in Genestoso cheese, a Spanish acid curd variety, throughout ripening. Food Control 2004, 15:271-279.
3. Bae S., Wuertz S. Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide. Applied and Environmental Microbiology 2009, 75:2940-2944.
4. Brescia C.C., Griffin S.M., Ware M.W., Varughese E.A., Egorov A.I., Villegas E.N. Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts. Applied and Environmental Microbiology 2009, 75:6856-6863.
5. Bickley J., Short J.K., McDowell D.G., Parkes H.C. Polymerase chain reaction (PCR) detection of Listeria monocytogenes in diluted milk and reversal of PCR inhibition caused by calcium ions. Letters in Applied Microbiology 1996, 22:153-158.
6. Brasil Ministério da Agricultura e do Abastecimento. Portaria n° 370, de 4 de setembro de 1997 1997, Regulamento técnico para fixação de identidade e qualidade do leite UHT (UAT), Diário Oficial da União [República Federativa do Brasil], Brasília, 8 set. 1997, Seção I.
7. Brasil Ministério da Agricultura, Pecuária e Abastecimento. Secretaria Nacional de Defesa Agropecuária 2003, Departamento de Defesa Animal. Métodos Analíticos Oficiais para Análises Microbiológicas Para Controle de Produtos de Origem Animal, Diário Oficial da União [República Federativa do Brasil], Brasília, 18 set. 2003.
8. Cawthorn D.M., Witthuhn R.C. Selective PCR detection of viable Enterobacter sakazakii cells utilizing propidium monoazide or ethidium bromide monoazide. Journal of Applied Microbiology 2008, 105:1178-1185.
9. Chen S., Wang F., Beaulieu J.C., Steins R.E., Ge B. Rapid detection of viable Salmonellae in produce by coupling propidium monoazide with Loup-Mediated isothermal amplification. Applied and Environmental Microbiology 2011, 77:4008-4016.
10. Chye F.Y., Abdullah A., Ayob M.K. Bacteriological quality and safety of raw milk in Malaysia. Food Microbiology 2004, 21:535-541.
11. Claeys W.L., Ludikhuyze L.R., Hendrickx M.E. Formation kinetics of hydroxymethylfurfural, lactulose and furosine in milk heated under isothermal and non-isothermal conditions. Journal of Dairy Research 2001, 68:287-301.
12. Contreras P.J., Urrutia H., Sossa K., Nocker A. Effect of PCR amplicon lenght on suppressing signals from membrane-compromised cells by propidium monoazide treatment. Journal of Microbiological Methods 2011, 87:89-95.
13. Elizaquível P., Sánchez G., Selma M.V., Aznar R. Application of propidium monoazide-qPCR to evaluate the ultrasonic inactivation of Escherichia coli O157:H7 in fresh-cut vegetable wash water. Food Microbiology 2012, 30:316-320.
14. Hammer P., Lembke F., Suhren G., Heeschen W. Characterization of a heat resistant mesophilic Bacillus species affecting quality of UHT-milk - a preliminary report. Kiel Milchwirt Forschungsber 1995, 47:297-305.
15. Herman L., Heyndrickx M., Waes G. Typing of Bacillus sporothermodurans and other Bacillus species isolated from milk by repetitive element sequence based PCR. Letters in Applied Microbiology 1998, 26:183-188.
16. Josefsen M.H., Löfström C., Hansen T.B., Christensen L.S., Olsen J.E., Hoorfar J. Rapid quantification of viable Campylobacter bacteria on chicken carcasses, using real-time PCR and propidium monoazide treatment, as a tool for quantification risk assessment. Applied and Environmental Microbiology 2010, 76:5097-5104.
17. Josephson K.L., Gerba C.P., Pepper I.L. Polymerase chain reaction detection of nonviable bacterial pathogens. Applied and Environmental Microbiology 1993, 59:3513-3515.
18. Klijn N., Herman L., Langeveld L., Vaerewijck M., Wagendorp A.A., Huemer I., Weerkamp A.H. Genotypical and phenotypical characterization of Bacillus sporothermodurans strains, surviving UHT sterilization. International Dairy Journal 1997, 7:421-428.
19. Liang N., Dong J., Luo L., Li Y. Detection of viable Salmonella in lettuce by propidium monoazide real-time PCR. Journal of Food Science 2011, 76:M234-M237.
20. Løvdal T., Hovda M.B., Björkblom B., Møller S.G. Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua. Journal of Microbiological Methods 2011, 85:164-169.
21. Mamlouk K., Macé S., Guilbaud M., Jaffreès E., Ferchichi M., Prévost H., Pilet M., Dousset X. Quantification of viable Brochothrix thermosphacta in cooked shrimp and salmon by real-time PCR. Food Microbiology 2012, 30:173-179.
22. Nocker A., Cheung C.Y., Camper A.K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. Journal of Microbiology Methods 2006, 67:310-320.
23. Nocker A., Sossa P., Burr M., Camper A.K. Use of propidium monoazide for live/dead distinction in microbial ecology. Applied and Environment Microbiology 2007, 73:5111-5117.
24. Nogva H.K., Drømtorp S.M., Nissen H., Rudi K. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. BioTechniques 2003, 34:804-813.
25. Pettersson B., Lembke F., Hammer P., Stackebrandt E., Priest F.G. Bacillus sporothermodurans, a new species producing highly heat-resistant endospores. International Journal of Systematic Bacteriology 1996, 46:759-764.
26. Rademaker J.L.W., de Bruijn F.J. Characterization and classification of microbes by REP-PCR genomic fingerprinting and computer-assisted pattern analysis. DNA Markers: Protocols, Applications, and Overviews 1997, Caetano-Anollés, G, Gresshoff, P.M, New York.
27. Rossen L., Norskov P., Holmstrom K., Rasmussen O.F. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA extraction solutions. International Journal of Food Microbiology 1992, 17:37-45.
28. Scheldeman P., Herman L., Foster S., Heyndrickx M. Bacillus sporothermodurans and other highly heat-resistant spore formers in milk. Journal of Applied Microbiology 2006, 101:542-555.
29. Scheldeman P., Herman L., Goris J., De Vos P., Heyndrickx M. Polymerase chain reaction identification of Bacillus sporothermodurans from dairy sources. Journal of Applied Microbiology 2002, 92:983-991.
30. Tabit F.T., Buys E. The effects of wet heat treatment on the structural and chemical components of Bacillus sporothermodurans spores. International Journal of Food Microbiology 2010, 140:207-213.
31. Tabit F.T., Buys E. Incidence and survival of Bacillus sporothermodurans during processing of UHT milk. British Food Journal 2011, 113:505-518.
32. Taskin B., Gozen A.G., Duran M. Selective quantification of viable Escherichia coli in biosolids by quantitative PCR with propidium monoazide modification. Applied and Environmental Microbiology 2011, 77:4329-4335.
33. Torkar K.G., Teger S.G. The Microbiological quality of raw milk after introducing the two day's milk collecting system. Acta Agriculturae Slovenica 2008, 92:61-74.
34. Vesper S., McKinstry C., Hartmann C., Neace M., Yoder S., Vesper A. Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA). Journal of Microbiological Methods 2008, 72:180-184.
35. Yáñez M.A., Nocker A., Soria-Soria E., Múrtula R., Martínez L., Catalán V. Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. Journal of Microbiology Methods 2011, 85:124-130.
36. Yang X., Badoni M., Gill C.O. Use of propidium monoazide and quantification PCR for differentiation of viable Escherichia coli from E. coli killed by mild or pasteurizing heat treatments. Food Microbiology 2011, 28:1478-1482.