In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells

Toxicology in Vitro

Volumn 27, Issue 2, 2013, Pages 864-873

  Hamzeh, Mahsa ^a   Sunahara, Geoffrey I ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Cell death; Comet assay; DNA damage; Flow cytometry; Nanotoxicology; Oxidative stress; Surface modification

Indexed keywords

NANOCOATING; NANOPARTICLE; POLYACRYLIC ACID; REACTIVE OXYGEN METABOLITE; TITANIUM DIOXIDE NANOPARTICLE; UNCLASSIFIED DRUG;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL VIABILITY; CHINESE HAMSTER; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; GENOTOXICITY; IN VITRO STUDY; LONG TERM EXPOSURE; LUNG FIBROBLAST; NANOANALYSIS; NANOCHEMISTRY; NANOTOXICOLOGY; NONHUMAN; PARTICLE SIZE; PHYSICAL CHEMISTRY; SURFACE PROPERTY;

ANIMALS; APOPTOSIS; CELL LINE; CELL SURVIVAL; COMET ASSAY; CRICETINAE; CRICETULUS; DNA DAMAGE; FIBROBLASTS; LUNG; METAL NANOPARTICLES; MICROSCOPY, ELECTRON, TRANSMISSION; NECROSIS; REACTIVE OXYGEN SPECIES; TITANIUM;

CRICETULUS GRISEUS;

EID: 84872696988     PISSN: 08872333     EISSN: 18793177     Source Type: Journal    
DOI: 10.1016/j.tiv.2012.12.018     Document Type: Article

References (66)

1. Ahmad I., Khan M.I., Patil G., Chauhan L.K.S. Evaluation of cytotoxic, genotoxic and inflammatory responses of micro- and nano-particles of granite on human lung fibroblast cell IMR-90. Toxicol. Lett. 2012, 208:300-307.
2. 2 nanoparticles by fibroblasts. Toxicol. In Vitro 2012, 26:469-479.
3. 2 to microalgae Pseudokirchneriella subcapitata. Sci. Total Environ. 2009, 407:1461-1468.
4. Asare N., Instances C., Sandberg W.J., Refsnes M., Schwarze P., Kruszewski M., Brunborg G. Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells. Toxicology 2012, 292:65-72.
5. Baan R., Straif K., Grosse Y., Secretan B., El Ghissassi F., Cogliano V. Carcinogenicity of carbon black, titanium dioxide, and talc. Lancet Oncol. 2006, 7:295-296.
6. Barillet S., Jugan M.L., Laye M., Leconte Y., Herlin-Boime N., Reynaud C., Carriere M. In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: cyto-, genotoxicity and oxidative stress. Toxicol. Lett. 2010, 198:324-330.
7. Borenfreund E., Puerner J.A. A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90). J. Tissue Cult. Methods 1984, 9:7-9.
8. Brunauer S., Emmett P.H., Teller E. Adsorption of gases in multimolecular layers. J. Am. Chem. Soc. 1938, 60(2):309-319.
9. Colvin V.L. The potential environmental impact of engineered nanomaterials. Nat. Biotechnol. 2003, 21:1166-1170.
10. Doak S.H., Manshian B., Jenkins G.J.S., Singh N. In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines. Mutat. Res. 2012, 745:104-111.
11. Donaldson K., Brown D., Clouter A., Duffin R., MacNee W., Renwick L., Tran L., Stone V. The pulmonary toxicology of ultrafine particles. J. Aerosol. Med. 2002, 15:213-220.
12. Donaldson K., Stone V., Seaton A., MacNee W. Ambient particle inhalation and the cardiovascular system: potential mechanisms. Environ. Health Perspect. 2001, 109(Suppl 4):523-527.
13. 6+. Free Radical Biol. Med. 2005, 39:1499-1511.
14. 2. Hum. Exp. Toxicol. 2009, 28:339-352.
15. Guichard Y., Schmit J., Darne C., Gate L., Goutet M., Rousset D., Rastoix O., Wrobel R., Witschger O., Martin A., Fierro V., Binet S. Cytotoxicity and genotoxicity of nanosized and microsized titanium dioxide and iron oxide particles in Syrian hamster embryo cells. Ann. Occup. Hyg. 2012, 56:631-644.
16. Gurr J.R., Wang A.S., Chen C.H., Jan K.Y. Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells. Toxicology 2005, 213:66-73.
17. Hext P.M., Tomenson J.A., Thompson P. Titanium dioxide: inhalation toxicology and epidemiology. Ann. Occup. Hyg. 2005, 49:461-472.
18. 2 in the rat. Int. J. Hyg. Environ. Health 2002, 205:239-244.
19. Horie M., Nishio K., Fujita K., Kato H., Endo S., Suzuki M., Nakamura A., Miyauchi A., Kinugasa S., Yamamoto K., Iwahashi H., Murayama H., Niki E., Yoshida Y. Cellular responses by stable and uniform ultrafine titanium dioxide particles in culture-medium dispersions when secondary particle size was 100nm or less. Toxicol. In Vitro 2010, 24:1629-1638.
20. 2 nanoparticles on A549 cells. J. Biomed. Nanotechnol. 2011, 7(1):22-23.
21. Kakinoki K., Yamane K., Teraoka R., Otsuka M., Matsuda Y. Effect of relative humidity on the photocatalytic activity of titanium dioxide and photostability of famotidine. J. Pharm. Sci. 2004, 93:582-589.
22. Kang S.J., Kim B.M., Lee Y.J., Chung H.W. Titanium dioxide nanoparticles trigger p53-mediated damage response in peripheral blood lymphocytes. Environ. Mol. Mutagen. 2008, 49:399-405.
23. Karlsson H.L., Cronholm P., Gustafsson J., Moller L. Copper oxide nanoparticles are highly toxic: a comparison between metal oxide nanoparticles and carbon nanotubes. Chem. Res. Toxicol. 2008, 21:1726-1732.
24. 2 nanoparticles induce oxidative stress and DNA damage leading to reduced viability of Eschrichia coli. Free Radical Biol. Med. 2011, 51:1872-1881.
25. Kumaravel T.S., Jha A.N. Reliable comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals. Mutat. Res. 2006, 605:7-16.
26. L'Azou B., Jorly J., On D., Sellier E., Moisan F., Fleury-Feith J., Cambar J., Brochard P., Ohayon-Courtès C. In vitro effects of nanoparticles on renal cells. Part Fibre Toxicol. 2008, 5:22.
27. Landsiedel R., Kapp M.D., Schulz M., Wiench K., Oesch F. Genotoxicity investigations on nanomaterials: methods, preparation and characterization of test material, potential artifacts and limitations-many questions, some answers. Mutat. Res. 2009, 681:241-258.
28. Lee J., Mahendra S., Alvarez P.J. Nanomaterials in the construction industry: a review of their applications and environmental health and safety considerations. ACS Nano 2010, 4:3580-3590.
29. Lewinski N., Colvin V., Drezek R. Cytotoxicity of nanoparticles. Small 2008, 4:26-49.
30. Limbach L.K., Li Y., Grass R.N., Brunner T.J., Hintermann M.A., Muller M., Gunther D., Stark W.J. Oxide nanoparticle uptake in human lung fibroblasts: effects of particle size, agglomeration, and diffusion at low concentrations. Environ. Sci. Technol. 2005, 39:9370-9376.
31. Liu S., Xu L., Zhang T., Ren G., Yang Z. Oxidative stress and apoptosis induced by nanosized titanium dioxide in PC12 cells. Toxicology 2010, 267:172-177.
32. Lomer M.C., Thompson R.P., Powell J.J. Fine and ultrafine particles of the diet: influence on the mucosal immune response and association with Crohn's disease. Proc. Nutr. Soc. 2002, 61:123-130.
33. Maurer-Jones M.A., Lin Y.S., Haynes C.L. Functional assessment of metal oxide nanoparticle toxicity in immune cells. ACS Nano 2010, 4:3363-3373.
34. Mazzola L. Commercializing nanotechnology. Nat. Biotechnol. 2003, 21:1137-1143.
35. McCarthy J.R., Weissleder R. Multifunctional magnetic nanoparticles for targeted imaging and therapy. Adv. Drug Deliv. Rev. 2008, 60:1241-1251.
36. Meng H., Xia T., George S., Nel A.E. A predictive toxicological paradigm for the safety assessment of nanomaterials. ACS Nano 2009, 3:1620-1677.
37. Nel A., Xia T., Madler L., Li N. Toxic potential of materials at the nanolevel. Science 2006, 311:622-627.
38. Nemmar A., Vanbilloen H., Hoylaerts M.F., Hoet P.H., Verbruggen A., Nemery B. Passage of intratracheally instilled ultrafine particles from the lung into the systemic circulation in hamster. Am. J. Respir. Crit. Care Med. 2001, 164:1665-1668.
39. Oberdörster G. Lung particle overload: implications for occupational exposures to particles. Regul. Toxicol. Pharm. 1995, 27:123-135.
40. Oberdörster G. Pulmonary effects of inhaled ultrafine particles. Int. Arch. Occup. Environ. Health 2001, 74:1-8.
41. Oberdörster G., Oberdörster E., Oberdörster J. Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ. Health. Perspect. 2005, 113:823-839.
42. Oberdörster G., Stone V., Donaldson K. Toxicology of nanoparticles: a historical perspective. Nanotoxicology 2007, 1:2-25.
43. Olive P.L., Durand R.E. Heterogeneity in DNA damage using the comet assay. Cytometry A 2005, 66:1-8.
44. Papageorgiou I., Brown C., Schins R., Singh S., Newson R., Davis S., Fisher J., Ingham E., Case C.P. The effect of nano- and micron-sized particles of cobalt-chromium alloy on human fibroblasts in vitro. Biomaterials 2007, 28:2946-2958.
45. Park E.J., Yi J., Chung K.H., Ryu D.Y., Choi J., Park K. Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells. Toxicol. Lett. 2008, 180:222-229.
46. Patil G., Khan M.I., Patel D.K., Sultana S., Prasad R., Ahmad I. Evaluation of cytotoxic, genotoxic and inflammatory responses of micro- and nano-particles of dolomite on human lung epithelial cells A549. Environ. Toxicol. Pharmacol. 2012, 34:436-445.
47. Paull R., Wolfe J., Hebert P., Sinkula M. Investing in nanotechnology. Nat. Biotechnol. 2003, 21:1144-1147.
48. 2/ZnS quantum dots for sentinel lymph node imaging with reduced toxicity. ACS Nano 2010, 4:2531-2538.
49. Pott F., Roller M. Carcinogenicity study with nineteen granular dusts in rats. Eur. J. Oncol. 2005, 10:249-281.
50. Rahman Q., Lohani M., Dopp E., Pemsel H., Jonas L., Weiss D.G., Schiffmann D. Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts. Environ. Health. Perspect. 2002, 110:797-800.
51. Renwick L.C., Brown D., Clouter A., Donaldson K. Increased inflammation and altered macrophage chemotactic responses caused by two ultrafine particle types. Occup. Environ. Med. 2004, 61:442-447.
52. Rivera Gil P., Oberdörster G., Elder A., Puntes V., Parak W.J. Correlating physico-chemical with toxicological properties of nanoparticles: the present and the future. ACS Nano 2010, 4:5527-5531.
53. Rothen-Rutishauser B.M., Schurch S., Haenni B., Kapp N., Gehr P. Interaction of fine particles and nanoparticles with red blood cells visualized with advanced microscopic techniques. Environ. Sci. Technol. 2006, 40:4353-4359.
54. Sayes C.M., Wahi R., Kurian P.A., Liu Y., West J.L., Ausman K.D., Warheit D.B., Colvin V.L. Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. Toxicol. Sci. 2006, 92:174-185.
55. Shukla R.K., Sharma V., Pandey A.K., Singh S., Sultana S., Dhawan A. ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells. Toxicol. In Vitro 2011, 25:231-241.
56. Sohaebuddin S.K., Thevenot P.T., Baker D., Eaton J.W., Tang L. Nanomaterial cytotoxicity is composition, size, and cell type dependent. Part Fibre Toxicol. 2010, 7:22.
57. Thevenot P., Hu W., Tang L. Surface chemistry influences implant biocompatibility. Curr. Top. Med. Chem. 2008, 8:270-280.
58. Trouiller B., Reliene R., Westbrook A., Solaimani P., Schiestl R.H. Titanium dioxide nanoparticles induce DNA damage and genetic instability in vivo in mice. Cancer Res. 2009, 69:8784-8789.
59. Valberg P.A., Bruch J., McCunney R.J. Are rat results from intratracheal instillation of 19 granular dusts a reliable basis for predicting cancer risk?. Regul. Toxicol. Pharmacol. 2009, 54:72-83.
60. 2 nanoparticles: studies on a human monoblastoid cell line. Toxicol. In Vitro 2008, 22:1689-1696.
61. Wang H., Joseph J.A. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radical Biol. Med. 1999, 27:612-616.
62. 2 particles in cultured human lymphoblastoid cells. Mutat. Res. 2007, 628:99-106.
63. 2 particle formulations: impact of surface treatments on particle toxicity. Toxicol. Sci. 2005, 88:514-524.
64. 2 particles: differential responses related to surface properties. Toxicology 2007, 230:90-104.
65. Xia T., Kovochich M., Liong M., Zink J.I., Nel A.E. Cationic polystyrene nanosphere toxicity depends on cell-specific endocytic and mitochondrial injury pathways. ACS Nano 2008, 2:85-96.
66. Xu A., Chai Y., Nohmi T., Hei T.K. Genotoxic responses to titanium dioxide nanoparticles and fullerene in gpt delta transgenic MEF cells. Part Fibre Toxicol. 2009, 6:3.