Engineering a substrate-specific cold-adapted subtilisin

Protein Engineering, Design and Selection

Volumn 17, Issue 2, 2004, Pages 149-156

  Tindbaek, Nikolaj ^a   Svendsen, Allan ^a   Oestergaard, Peter Rahbek ^a   Draborg, Henriette ^a  

^a NOVOZYMES A S   (Denmark)

Author keywords

Cold adaptation; Molecular dynamics; Protein engineering; Protein stability; Psychrophilic enzymes

Indexed keywords

ANILIDE; N SUCCINYLALANYLALANYLPROLYLPHENYLALANINE 4 NITROANILIDE; SUBTILISIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; COLD ACCLIMATIZATION; COMPUTER MODEL; CONTROLLED STUDY; DIFFERENTIAL SCANNING CALORIMETRY; EGGERTHELLA LENTA; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME ENGINEERING; ENZYME MODIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; HYBRID; LOW TEMPERATURE; MEASUREMENT; MOLECULAR DYNAMICS; NONHUMAN; PH; PREDICTION; PRIORITY JOURNAL; ROOM TEMPERATURE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SIMULATION; TEMPERATURE; TEMPERATURE MEASUREMENT; THERMOSTABILITY;

BACILLUS; BINDING SITES; COLD; ENZYME ACTIVATION; ENZYME STABILITY; HEAT; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES; STRUCTURAL HOMOLOGY, PROTEIN; SUBSTRATE SPECIFICITY; SUBTILISIN;

BACILLUS LENTUS; EGGERTHELLA LENTA;

EID: 1842852031     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzh019     Document Type: Article

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