A cold-adapted protease engineered by experimental evolution system

Journal of Biochemistry

Volumn 126, Issue 4, 1999, Pages 689-693

  Taguchi, Seiichi ^a,c   Ozaki, Akiyoshi ^a   Nonaka, Takamasa ^b   Mitsui, Yukio ^b   Momose, Haruo ^a  

^a TOKYO UNIVERSITY OF SCIENCE   (Japan)

^b NAGAOKA UNIVERSITY OF TECHNOLOGY   (Japan)

^c INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

Author keywords

Cold adaptation; Evolutionary engineering; Kinetic properties; Subtilisin BPN'; Thermal stability

Indexed keywords

ALANINE; MUTANT PROTEIN; PROTEINASE; SUBTILISIN; THREONINE; VALINE;

ARTICLE; COLD ACCLIMATIZATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENE MUTATION; GENETIC ANALYSIS; HYDROLYSIS; MOLECULAR EVOLUTION; MOLECULAR MODEL; MUTAGENESIS; PROTEIN TERTIARY STRUCTURE; THERMOSTABILITY;

ESCHERICHIA COLI; FELIS CATUS;

EID: 0032708454     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022504     Document Type: Article

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