Chymotrypsin selectively digests β-lactoglobulin in whey protein isolate away from enzyme optimal conditions: Potential for native α-lactalbumin purification

Journal of Dairy Research

Volumn 80, Issue 1, 2013, Pages 14-20

  Lisak, Katarina ^a   Toro Sierra, Jose ^b   Kulozik, Ulrich ^b   Bozanic, Rajka ^a   Cheison, Seronei Chelulei ^b,c  

^a UNIVERSITY OF ZAGREB   (Croatia)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c MASENO UNIVERSITY   (Kenya)

Author keywords

chymotrypsin; lactalbumin recovery; lactoglobulin; Bowman Birk inhibitor; chicken egg white inhibitor; selective whey protein hydrolysis

Indexed keywords

ALPHA-CHYMOTRYPSIN; CHYMOTRYPSIN; CHYMOTRYPSIN A; LACTALBUMIN; LACTOGLOBULIN; MILK PROTEIN; PROTEINASE INHIBITOR; WHEY PROTEIN;

ANIMAL; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; HEAT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; ISOLATION AND PURIFICATION; METABOLISM; MILK; PH;

ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHYMOTRYPSIN; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; LACTALBUMIN; LACTOGLOBULINS; MILK; MILK PROTEINS; PROTEASE INHIBITORS; SUBSTRATE SPECIFICITY;

EID: 84872444136     PISSN: 00220299     EISSN: 14697629     Source Type: Journal    
DOI: 10.1017/S0022029912000416     Document Type: Article

References (31)

1. Adler-Nissen J 1986 Enzymic Hydrolysis of Food Proteins. London: Elsevier Applied Science Publishers Ltd
2. Alomirah HF & Alli I 2004 Separation and characterization of β-lactoglobulin and α-lactalbumin from whey and whey protein preparations. International Dairy Journal 14 411-419 (Pubitemid 38345664)
3. Bramaud C, Aimar P & Daufin G 1997 Optimisation of a whey protein fractionation process based on the selective precipitation of α-lactalbumin. Le Lait 77 411-423 (Pubitemid 127370256)
4. Chatterton DEW, Smithers G, Roupas P & Brodkorb A 2006 Bioactivity of β-lactoglobulin and α-lactalbumin-technological implications for processing. International Dairy Journal 16 1229-1240 (Pubitemid 44348873)
5. Cheang B & Zydney AL 2004 A two-stage ultrafiltration process for fractionation of whey protein isolate. Journal of Membrane Science 231 159-167 (Pubitemid 38221482)
6. Cheison SC, Leeb E, Toro-Sierra J & Kulozik U 2011 Influence of hydrolysis temperature and pH on the selective trypsinolysis of whey proteins and potential recovery of native alpha-lactalbumin. International Dairy Journal 21 166-171
7. Cheison SC, Schmitt M, Leeb E, Letzel T & Kulozik U 2010 Influence of the temperature and the degree of hydrolysis on the peptide composition of trypsin hydrolysates of β-lactoglobulin: analysis by LC-ESI-TOF/MS. Food Chemistry 121 457-467
8. Cheison SC, Wang Z & Xu S-Y 2007 Multivariate strategy in screening of enzymes to be used for whey protein hydrolysis in an enzymatic membrane reactor. International Dairy Journal 17 393-402 (Pubitemid 46048666)
9. Creamer LK, Nilsson HC, Paulsson MA, Coker CJ, Hill JP & Jimenez-Flores R 2004 Effect of genetic variation on the tryptic hydrolysis of bovine β-lactoglobulin A, B, and C. Journal of Dairy Science 87 4023-4032 (Pubitemid 39606233)
10. Custodio MF, Goulart AJ, Marques DP, Giordano RC, Giordano RLC & Monti R 2005 Hydrolysis of cheese whey proteins with trypsin, chymotrypsin and carboxypeptidase. Alimentação e Nutrição 16 105-109
11. Galvão CM, Silva AF, Custódio MF, Monti R & Giordano RL 2001 Controlled hydrolysis of cheese whey proteins using trypsin and alphachymotrypsin. Applied Biochemistry and Biotechnology 91 761-776 (Pubitemid 32434040)
12. Gésan-Guiziou G, Daufin G, Timmer M, Allersma D & Van Der Horst C 1999 Process steps for the preparation of purified fractions of α- lactalbumin and β-lactoglobulin from whey protein concentrates. Journal of Dairy Research 66 225-236 (Pubitemid 29228713)
13. Kamau SM, Cheison SC, Chen W, Liu X-M & Lu R-R 2010 Alphalactalbumin: its production technologies and bioactive peptides. Comprehensive Reviews in Food Science and Food Safety 9 197-212
14. Kennedy AR 1998 The Bowman-Birk inhibitor from soybeans as an anticarcinogenic agent. American Journal of Clinical Nutrition 68 1406S-1412S (Pubitemid 28550921)
15. Kiesner C, Clawin-Rädecker I, Meisel H & Buchheim W 2000 Manufacturing of α-lactalbumin-enriched whey systems by selective thermal treatment in combination with membrane processes. Le Lait 80 99-111
16. Konrad G & Kleinschmidt T 2008 A new method for isolation of native a-lactalbumin from sweet whey. International Dairy Journal 18 47-54 (Pubitemid 350123045)
17. Kristiansen KR, Otte J, Ipsen R & Qvist KB 1998 Large-scale preparation of β-lactoglobulin A and B by ultrafiltration and ion-exchange chromatography. International Dairy Journal 8 113-118 (Pubitemid 28434030)
18. Lönnerdal B & Lien EL 2003 Nutritional and physiologic significance of α-lactalbumin in infants. Nutrition Reviews 61 295-305 (Pubitemid 37259920)
19. Mailliart P & Ribadeau-Dumas B 1988 Preparation of β-lactoglobulin and p-lactoglobulin-free proteins from whey retentate by NaCl salting out at low pH. Journal of Food Science 53 743-745
20. Manji B, Hill A, Kakuda Y & Irvine DM 1985 Rapid separation of milk whey proteins by anion exchange chromatography. Journal of Dairy Science 68 3176-3179
21. Mehra RK & Donnelly WJ 1993 Fractionation of whey protein components through a large pore size, hydrophilic cellulose membrane. Journal of Dairy Research 60 89-97
22. Monaci L, Tregoat V, Hengel AJ & Anklam E 2006 Milk allergens, their characteristic and their detection in food: a review. European Food Research and Technology 223 149-179 (Pubitemid 43760716)
23. N'Negue MA, Miclo L, Girardet JM, Campagna S, Mollé D & Gaillard JL 2006 Proteolysis of bovine α-lactalbumin by thermolysin during thermal denaturation. International Dairy Journal 16 1157-1167 (Pubitemid 43870424)
24. Outinen M, Tossavainen O & Syväoja EL 1996 Chromatographic fractionation of α-lactalbumin and β-lactoglobulin with polystyrenic strongly basic anion exchange resins. Lebensmittel-Wissenschaft und- Technologie 29 340-343 (Pubitemid 126163897)
25. Permyakov EA & Berliner LJ 2000 α-Lactalbumin: structure and function. FEBS Letters 473 269-274
26. Pintado ME, Pintado AE & Malcata FX 1999 Controlled whey protein hydrolysis using two alternative proteases. Journal of Food Engineering 42 1-13
27. Schmidt DG & Poll JK 1991 Enzymatic hydrolysis of whey proteins. Hydrolysis of α-lactalbumin and β-lactoglobulin in buffer solutions by proteolytic enzymes. Netherlands Milk and Dairy Journal 45 225-240
28. Smithers GW 2008 Whey and whey proteins - from 'gutter-to-gold'. International Dairy Journal 18 695-704
29. Sweeney PJ & Walker JM 1993 Proteolytic enzymes for peptide production. In Enzymes of Molecular Biology Vol. 16, pp. 277-303 (Ed. MM Burrell) Berlin: Springer-Verlag GmbH
30. Tanford C, Bunville LG & Nozaki Y 1959 The reversible transformation of β-lactoglobulin at pH 7·51. Journal of the American Chemical Society 81 4032-4036
31. Tolkach A, Steinle S & Kulozik U 2005 Optimization of thermal pretreatment conditions for the separation of native α-lactalbumin from whey protein concentrates by means of selective denaturation of β-lactoglobulin. Journal of Food Science 70 E557-E566 (Pubitemid 43015958)