EZH2 is regulated by ERK/AKT and targets integrin alpha2 gene to control Epithelial-Mesenchymal Transition and anoikis in colon cancer cells

International Journal of Biochemistry and Cell Biology

Volumn 45, Issue 2, 2013, Pages 243-254

  Ferraro, Angelo ^a   Mourtzoukou, Despoina ^b   Kosmidou, Vivian ^a   Avlonitis, Spiros ^b   Kontogeorgos, George ^b   Zografos, George ^b   Pintzas, Alexander ^a  

^a INSTITUTE OF BIOLOGY   (Greece)

^b G GENNIMATAS GENERAL HOSPITAL   (Greece)

Author keywords

Anoikis; EMT; EZH2; ITG 2; Metastasis

Indexed keywords

ALPHA2 INTEGRIN; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN C JUN; PROTEIN KINASE B; TRANSCRIPTION FACTOR AP 1; TRANSCRIPTION FACTOR EZH2; TRANSCRIPTION FACTOR FRA 1;

ANOIKIS; ARTICLE; CANCER CELL CULTURE; CANCER INVASION; CELL LEVEL; CHROMATIN IMMUNOPRECIPITATION; COLON CANCER; COLON CARCINOGENESIS; CONTROLLED STUDY; ENZYME INHIBITION; EPIGENETICS; EPITHELIAL MESENCHYMAL TRANSITION; GENE CONTROL; GENE EXPRESSION PROFILING; GENE OVEREXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; HUMAN TISSUE; INTRACELLULAR SIGNALING; METASTASIS; NUCLEOTIDE BINDING SITE; PROMOTER REGION; PROTEIN TARGETING; REGULATOR GENE; RNA ANALYSIS; TRANSCRIPTION REGULATION; TUMOR MICROENVIRONMENT;

ANOIKIS; CACO-2 CELLS; CELL MOVEMENT; CELL SURVIVAL; CELL TRANSFORMATION, NEOPLASTIC; COLON; COLONIC NEOPLASMS; EPITHELIAL-MESENCHYMAL TRANSITION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE KNOCKDOWN TECHNIQUES; HCT116 CELLS; HUMANS; INTEGRIN ALPHA2; INTESTINAL MUCOSA; LYMPHATIC METASTASIS; POLYCOMB REPRESSIVE COMPLEX 2; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; PROTEIN TRANSPORT; PROTO-ONCOGENE PROTEINS C-AKT; RNA, SMALL INTERFERING; TRANSCRIPTION FACTOR AP-1;

EID: 84872344025     PISSN: 13572725     EISSN: 18785875     Source Type: Journal    
DOI: 10.1016/j.biocel.2012.10.009     Document Type: Article

References (45)

1. A.P. Bracken, and K. Helin Polycomb group proteins: navigators of lineage pathways led astray in cancer Nature Reviews Cancer 9 2009 773 784
2. A. Budhu, M. Forgues, Q.H. Ye, H.L. Jia, P. He, and K.A. Zanetti Prediction of venous metastases, recurrence, and prognosis in hepatocellular carcinoma based on a unique immune response signature of the liver microenvironment Cancer Cell 10 2006 99 111
3. A. Cano, M.A. Pérez-Moreno, I. Rodrigo, A. Locascio, M.J. Blanco, and M.G. del Barrio The transcription factor Snail controls epithelial- mesenchymal transitions by repressing E-cadherin expression Nature Cell Biology 2 2000 76 83
4. Q. Cao, J. Yu, S.M. Dhanasekaran, J.H. Kim, R.S. Mani, and S.A. Tomlins Repression of E-cadherin by the polycomb group protein EZH2 in cancer Oncogene 27 2008 7274 7284
5. R. Cao, L. Wang, H. Wang, L. Xia, H. Erdjument-Bromage, and P. Tempst Role of histone H3 lysine 27 methylation in Polycomb-group silencing Science 298 2002 1039 1043
6. U.R. Chandran, C. Ma, R. Dhir, M. Bisceglia, M. Lyons-Weiler, and W. Liang Gene expression profiles of prostate cancer reveal involvement of multiple molecular pathways in the metastatic process BMC Cancer 12 2007 7 64
7. A.S. Cheng, S.S. Lau, Y. Chen, Y. Kondo, M.S. Li, and H. Feng EZH2-mediated concordant repression of Wnt antagonists promotes β-catenin-dependent hepatocarcinogenesis Cancer Research 71 2011 4028 4039
8. U. Doehn, C. Hauge, S.R. Frank, C.J. Jensen, K. Duda, and J.V. Nielsen RSK is a principal effector of the RAS-ERK pathway for eliciting a coordinate promotile/invasive gene program and phenotype in epithelial cells Molecular Cell 35 2009 511 522
9. H. Ellinger-Ziegelbauer, K. Kelly, and U. Siebenlist Cell cycle arrest and reversion of Ras-induced transformation by a conditionally activated form of mitogen-activated protein kinase kinase kinase 3 Molecular and Cellular Biology 19 1999 3857 3868
10. J.Y. Fang, and B.C. Richardson The MAPK signalling pathways and colorectal cancer Lancet Oncology 6 2005 322 327
11. S.M. Frisch, and H. Francis Disruption of epithelial cell-matrix interactions induces apoptosis Journal of Cell Biology 124 1994 619 626
12. S. Fujii, K. Tokita, N. Wada, K. Ito, C. Yamauchi, and Y. Ito MEK-ERK pathway regulates EZH2 overexpression in association with aggressive breast cancer subtypes Oncogene 30 2011 4118 4128
13. M.E. Gonzalez, X. Li, K. Toy, M. DuPrie, A.C. Ventura, and M. Banerjee Downregulation of EZH2 decreases growth of estrogen receptor-negative invasive breast carcinoma and requires BRCA1 Oncogene 28 2009 843 853
14. R.O. Hynes Integrins: bidirectional, allosteric signaling machines Cell 110 2002 673 687
15. J. Ivaska, H. Reunanen, J. Westermarck, L. Koivisto, V.M. Kähäri, and J. Heino Integrin alpha2beta1 mediates isoform-specific activation of p38 and upregulation of collagen gene transcription by a mechanism involving the alpha2 cytoplasmic tail Journal of Cell Biology 147 1999 401 416
16. S. Jones, W.D. Chen, G. Parmigiani, F. Diehl, N. Beerenwinkel, and T. Antal Comparative lesion sequencing provides insights into tumor evolution Proceedings of the National Academy of Sciences of the United States of America 105 2008 4283 4288
17. T. Joyce, D. Cantarella, C. Isella, E. Medico, and A. Pintzas A molecular signature for epithelial to mesenchymal transition in a human colon cancer cell system is revealed by large-scale microarray analysis Clinical and Experimental Metastasis 26 2009 569 587
18. M. Kalogeropoulou, A. Voulgari, V. Kostourou, R. Sandaltzopoulos, R. Dikstein, and I. Davidson TAF4b and Jun/activating protein-1 collaborate to regulate the expression of integrin alpha6 and cancer cell migration properties Molecular Cancer Research 8 2010 554 568
19. C.G. Kleer, Q. Cao, S. Varambally, R. Shen, I. Ota, and S.A. Tomlins EZH2 is a marker of aggressive breast cancer and promotes neoplastic transformation of breast epithelial cells Proceedings of the National Academy of Sciences of the United States of America 30 2003 11606 11611
20. C. Lu, H.D. Han, L.S. Mangala, R. Ali-Fehmi, C.S. Newton, and L. Ozbun Regulation of tumor angiogenesis by EZH2 Cancer Cell 18 2010 185 197
21. F. Mainiero, C. Murgia, K.K. Wary, A.M. Curatola, A. Pepe, and M. Blumemberg The coupling of alpha6beta4 integrin to Ras-MAP kinase pathways mediated by Shc controls keratinocyte proliferation EMBO Journal 16 1997 2365 2375
22. R. Margueron, and D. Reinberg The Polycomb complex PRC2 and its mark in life Nature 469 2011 343 349
23. S.D. Markowitz, and M.M. Bertagnolli Molecular origins of cancer: molecular basis of colorectal cancer New England Journal of Medicine 361 2009 2449 2460
24. I. Mazon-Peláez, M. Kalogeropoulou, A. Ferraro, A. Voulgari, T. Pankotai, and I. Boros Oncogenic RAS alters the global and gene-specific histone modification pattern during epithelial-mesenchymal transition in colorectal carcinoma cells International Journal of Biochemistry and Cell Biology 42 2010 911 920
25. J. Min, A. Zaslavsky, G. Fedele, S.K. McLaughlin, E.E. Reczek, and T. De Raedt An oncogene-tumor suppressor cascade drives metastatic prostate cancer by coordinately activating Ras and nuclear factor-kappaB Nature Medicine 16 2010 286 294
26. E.J. Morris, N.J. Maughan, D. Forman, and P. Quirke Who to treat with adjuvant therapy in Dukes B/stage II colorectal cancer? The need for high quality pathology Gut 56 2007 1419 1425
27. D.X. Nguyen, P.D. Bos, and J. Massagué Metastasis: from dissemination to organ-specific colonization Nature Reviews Cancer 9 2009 274 284
28. C.S. Potten Kinetics and possible regulation of crypt cell populations under normal and stress conditions Bulletin du Cancer 62 1975 419 430
29. A. Provenzani, R. Fronza, F. Loreni, A. Pascale, and A. Quattrone Global alterations in mRNA polysomal recruitment in a cell model of colorectal cancer progression to metastasis Carcinogenesis 27 2006 1323 1333
30. 1 integrin is a metastasis suppressor in mouse models and human cancer Journal of Clinical Investigation 121 2011 226 237
31. G. Ren, S. Baritaki, H. Marathe, J. Feng, S. Park, and S. Beach Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer Cancer Research 72 2012 3091 3104
32. M. Roberts, K. Drosopoulos, G. Vasileiou, M. Stricker, E. Taoufik, and C. Maercker Microarray analysis of the differential transformation mediated by kirsten and harvey ras oncogenes in a human colon adenocarcinoma cell line International Journal of Cancer 118 2006 616 627
33. Z. Shaikhibrahim, A. Lindstrot, R. Buettner, and N. Wernert Analysis of laser-microdissected prostate cancer tissues reveals potential tumor markers International Journal of Molecular Medicine 28 2011 605 611
34. L.M. Shaw, I. Rabinovitz, H.H. Wang, A. Toker, and A.M. Mercurio Activation of phosphoinositide 3-OH kinase by the alpha6beta4 integrin promotes carcinoma invasion Cell 91 1997 949 960
35. S. Shin, C.A. Dimitri, S.O. Yoon, W. Dowdle, and J. Blenis ERK2 but not ERK1 induces epithelial-to-mesenchymal transformation via DEF motif-dependent signaling events Molecular Cell 38 2010 114 127
36. C.D. Simpson, K. Anyiwe, and A.D. Schimmer Anoikis resistance and tumor metastasis Cancer Letters 272 2008 177 185
37. E. Smith, and A. Shilatifard The chromatin signaling pathway: diverse mechanisms of recruitment of histone-modifying enzymes and varied biological outcomes Molecular Cell 40 2010 689 701
38. J. Tan, X. Yang, L. Zhuang, X. Jiang, W. Chen, and P.L. Lee Pharmacologic disruption of polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells Genes and Development 21 2007 1050 1063
39. J.P. Thiery Epithelial-mesenchymal transitions in tumour progression Nature Reviews Cancer 2 2002 442 454
40. S. Varambally, S.M. Dhanasekaran, M. Zhou, T.R. Barrette, C. Kumar-Sinha, and M.G. Sanda The polycomb group protein EZH2 is involved in progression of prostate cancer Nature 419 2002 624 629
41. A. Voulgari, S. Voskou, L. Tora, I. Davidson, T. Sasazuki, and S. Shirasawa TATA box-binding protein-associated factor 12 is important for RAS-induced transformation properties of colorectal cancer cells Molecular Cancer Research 6 2008 1071 1083
42. B. Willipinski-Stapelfeld, S. Riethdorf, V. Assmann, U. Woelfl, T. Rau, and G. Sauter Changes in cytoskeletal protein composition indicative of an epithelial-mesenchymal transition in human micrometastatic and primary breast carcinoma cells Clinical Cancer Research 11 2005 8006 8014
43. Z.L. Wu, S.S. Zheng, Z.M. Li, Y.Y. Qiao, M.Y. Aau, and Q. Yu Polycomb protein EZH2 regulates E2F1-dependent apoptosis through epigenetically modulating Bim expression Cell Death and Differentiation 17 2010 801 810
44. J. Yu, J. Yu, D.R. Rhodes, S.A. Tomlins, X. Cao, and G. Chen A polycomb repression signature in metastatic prostate cancer predicts cancer outcome Cancer Research 67 2007 10657 10663
45. M.M. Zutter, S.A. Santoro, W.D. Staatz, and Y.L. Tsung Re-expression of the alpha 2 beta 1 integrin abrogates the malignant phenotype of breast carcinoma cells Proceedings of the National Academy of Sciences of the United States of America 92 1995 7411 7415