Engineering of α1-antitrypsin variants with improved specificity for the proprotein convertase furin using site-directed random mutagenesis

Protein Engineering, Design and Selection

Volumn 26, Issue 2, 2013, Pages 123-131

  Hada, Koichiro ^a   Isshiki, Kinuka ^a   Matsuda, Shinya ^a   Yuasa, Keizo ^a   Tsuji, Akihiko ^a  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

Author keywords

1 antitrypsin; 1 PDX; furin; PACE4; serpin

Indexed keywords

BACTERIAL TOXIN; BONE MORPHOGENETIC PROTEIN-4; CELL DIFFERENTIATION; CONSENSUS MOTIF; ECTOPIC EXPRESSIONS; EX-VIVO; FURIN; IN-VITRO; PACE4; PROPROTEIN CONVERTASE FURIN; RANDOM MUTAGENESIS; REACTIVE SITE; SELECTIVE INHIBITORS; SERPIN; SODIUM DODECYL SULFATE; SUBTILISIN-LIKE PROPROTEIN CONVERTASE; TUMOR GROWTH; VIRUS REPLICATION;

SODIUM; VIRUSES;

SODIUM SULFATE;

ALPHA 1 ANTITRYPSIN; ARGININE; BONE MORPHOGENETIC PROTEIN 4; DODECYL SULFATE SODIUM; FURIN;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; EX VIVO STUDY; GENETIC TRANSFECTION; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN MOTIF; RAT; SITE DIRECTED MUTAGENESIS;

ALPHA 1-ANTITRYPSIN; AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; ANIMALS; ANTITHROMBIN III; BONE MORPHOGENETIC PROTEIN 4; CATALYTIC DOMAIN; CERCOPITHECUS AETHIOPS; COS CELLS; FURIN; HEK293 CELLS; HUMANS; MUTAGENESIS, SITE-DIRECTED; PROPROTEIN CONVERTASE 5; PROPROTEIN CONVERTASES; PROTEIN BINDING; PROTEIN PRECURSORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STABILITY; PROTEOLYSIS; RATS; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); RATTUS;

EID: 84872315938     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs091     Document Type: Article

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