Cysteine scanning mutagenesis of transmembrane helix 3 of a brain glutamate transporter reveals two conformationally sensitive positions

Journal of Biological Chemistry

Volumn 288, Issue 2, 2013, Pages 964-973

  Silverstein, Nechama ^a   Crisman, Thomas J ^b   Forrest, Lucy R ^b   Kanner, Baruch I ^a  

^a HEBREW UNIVERSITY   (Israel)

^b MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID RESIDUES; ARCHAEAL; COMPUTATIONAL MODELING; CYSTEINE-SCANNING MUTAGENESIS; ELECTROSTATIC REPULSION; FULLY PROTECTED; GLUTAMATE TRANSPORTERS; INTRACELLULAR SIDE; MEMBRANE-IMPERMEANT; METHANETHIOSULFONATE; POSITIVE CHARGES; SODIUM IONS; STRUCTURAL WORKS; SULFHYDRYL; TRANSMEMBRANE HELICES; TRANSPORT ACTIVITY; TRIMERIZATION;

METAL IONS; SODIUM;

AMINO ACIDS;

2 AMINOETHYL METHANETHIOSULFONATE; CYSTEINE; GLIAL GLUTAMATE TRANSPORTER 1; GLUTAMATE RECEPTOR; GLUTAMIC ACID; N ETHYLMALEIMIDE; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; BRAIN; CELL GROWTH; CHEMICAL MODIFICATION; CONTROLLED STUDY; CYSTEINE SCANNING MUTAGENESIS; HELA CELL; MOLECULAR CLONING; MUTANT; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; TRANSMEMBRANE HELIX 3;

AMINO ACID TRANSPORT SYSTEM X-AG; BRAIN; CYSTEINE; MEMBRANE PROTEINS; MODELS, MOLECULAR; MUTAGENESIS; PROTEIN CONFORMATION; SULFHYDRYL REAGENTS;

ARCHAEA;

EID: 84872313828     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.403576     Document Type: Article

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