Quantum dot immunocytochemical localization of somatostatin in somatostatinoma by widefield epifluorescence, superresolution light, and immunoelectron microscopy

Journal of Histochemistry and Cytochemistry

Volumn 60, Issue 11, 2012, Pages 832-843

  Killingsworth, Murray C ^a,b   Lai, Ken ^a,c   Wu, Xiaojuan ^a   Yong, Jim L C ^a,c   Lee, C Soon ^a  

^a UNIVERSITY OF WESTERN SYDNEY   (Australia)

^b UNIVERSITY OF NEW SOUTH WALES   (Australia)

^c UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Electron microscopy; Immunocytochemistry; Quantum dot; Somatostatin

Indexed keywords

EPOXY RESIN; PERIODATE SODIUM; QUANTUM DOT; SOMATOSTATIN; DIAGNOSTIC AGENT;

ADULT; ANTIBODY LABELING; ANTIGENICITY; ARTICLE; CELL GRANULE; CELLULAR PARAMETERS; CORRELATIONAL STUDY; CYTOPLASM; ELECTRON MICROSCOPE; EPIFLUORESCENCE MICROSCOPY; FEMALE; FLUORESCENCE MICROSCOPE; GRANULE CELL; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; MICROSCOPE; MICROSCOPY; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SECRETORY GRANULE; SOMATOSTATINOMA; SUPER RESOLUTION LIGHT MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY; TUMOR CELL; FLUORESCENCE MICROSCOPY; METHODOLOGY; PATHOLOGY; VATER PAPILLA;

ADULT; AMPULLA OF VATER; FEMALE; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, FLUORESCENCE; MICROSCOPY, IMMUNOELECTRON; QUANTUM DOTS; SOMATOSTATIN; SOMATOSTATINOMA;

EID: 84872047755     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/0022155412459856     Document Type: Article

References (37)

1. Antoine JC, Avrameas S, Gonatas NK, Stieber A, Gonatas JO. 1974. Plasma membrane and internalized immunoglobulins of lymph node cells stained with conjugates of antibody or its Fab fragments with horseradish peroxidise. J Cell Biol. 63:12-23.
2. Brorson SH. 1996. Improved immunogold labeling of epoxy sections by the use of propylene oxide as an additional agent in dehydration, infiltration and embedding. Micron 27:345-353.
3. Brorson SH. 1997. How to examine the antigen-damaging effect of sodium ethoxide on deplasticized epoxy sections. J Histochem Cytochem. 45:143-146.
4. Brorson SH. 1998a. The combination of high-accelerator epoxy resin and antigen retrieval to obtain more intense immunolabeling on epoxy sections than on LR-White sections for large proteins. Micron 29:89-95.
5. Brorson SH. 1998b. Comparison of the immunogold labeling of single light chains and whole immunoglobulins with antikappa on LR White and epoxy sections. Micron 29:439-443.
6. Brorson SH. 2004. A method for measurement of the efficiency of immunogold labeling of epoxy embedded proteins subjected to different retrieval techniques. Micron 35:619-621.
7. Brorson SH, Reinholt FP. 2008. The intensity of immunogold labeling of deplasticized acrylic sections compared to deplasticized epoxy sections-theoretical deductions and experimental data. Micron 39:144-150.
8. Brorson SH, Skjørten F. 1995. Mechanism for antigen detection on deplasticised epoxy sections. Micron 26:301-310.
9. Brorson SH, Strøm EH. 1998. A new immunoelectron microscopy approach for the detection of immunoglobulin and complement deposits in epoxy-embedded renal biopsies. Ultrastruct Pathol. 22:449-457.
10. D'Alessandro D, Mattii L, Moscata S, Bernardini N, Segnani C, Dolfi A, Bianchi F. 2004. Immunohistochemical demonstration of the small GTPase RhoA on epoxy-resin embedded sections. Micron 35:287-296.
11. De Valck V, Renmans W, Segers E, Leunissen J, De Waele M. 1991. Light microscopical detection of leucocyte cell surface antigens with a one-nanometer gold probe. Histochemistry 95:483-490.
12. Donini U, Casanova S, Zucchelli P, Linke RP. 1989. Immunoelectron microscopic classification of amyloid in renal biopsies. J Histochem Cytochem. 37:1101-1106.
13. Gao X, Cui Y, Levenson RM, Chung LWK, Nie S. 2004. In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol. 22:969-976.
14. Giepmans BNG, Deerinck TJ, Smarr BL, Jones YZ, Ellisman MH. 2005. Correlated light and electron microscopic imaging of multiple endogenous proteins using quantum dots. Nat Methods. 2:743-749.
15. Groos S, Reale E, Luciano L. 2001. Re-evaluation of epoxy resin sections for light and electron microscopic immunostaining. J Histochem Cytochem. 49:397-406.
16. Gustafsson MGL, Agard DA, Sedat JW. 2000. Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination. Proc SPIE. 3919:141-150.
17. Hann CR, Springett MJ, Johnson DH. 2001. Antigen retrieval of basement membrane proteins from archival eye tissues. J Histochem Cytochem. 49:475-482.
18. Hearn SA, Silver MM, Sholdice JA. 1985. Immunoelectron microscopic labeling of immunoglobulin in plasma cells after osmium fixation and epoxy embedding. J Histochem Cytochem. 33:1212-1218.
19. Hell SW. 2007. Far-field optical nanoscopy. Science 316:1153-1158.
20. Hutter E, Maysinger D. 2011. Gold nanoparticles and quantum dots for bioimaging. Microsc Res Tech. 74:592-604.
21. Nisman R, Dellaire G, Ren Y, Li R, Bazett-Jones DP. 2004. Application of quantum dots as probes for correlative fluorescence, conventional, and energy-filtered transmission electron microscopy. J Histochem Cytochem. 52:13-18.
22. Meyer DA, Oliver JA, Albrecht RM. 2010. Colloidal palladium particles of different shapes for electron microscopy labeling. Microsc Microanal. 16:33-42.
23. Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T. 2008. Quantum dots versus organic dyes as fluorescent labels. Nat Methods. 9:763-772.
24. Robinson JM, Vandré DD. 1997. Efficient immunocytochemical labeling of leukocyte microtubules with FluoroNanogold: an important tool for correlative microscopy. J Histochem Cytochem. 45:631-642.
25. Roth J, Bendayan M, Orci L. 1980. FITC-protein A-gold complex for light and electron microscopic immunocytochemistry. J Histochem Cytochem. 28:55-57.
26. Schwarz H, Humbel BM. 2007. Correlative light and electron microscopy using immunolabeled resin sections. In: Kuo J, editor. Methods in molecular biology: Vol. 369. Electron microscopy: methods and protocols. 2nd ed. Totowa, NJ: Humana. p. 229-256.
27. Stirling JW, Graff PS. 1995. Antigen unmasking for immunoelectron microscopy: labeling is improved by treating with sodium ethoxide or sodium metaperiodate, then heating on retrieval medium. J Histochem Cytochem. 43:115-123.
28. Tassa C, Duffner JL, Lewis TA, Weissleder R, Schreiber SL, Koehler AN, Shaw SY. 2010. Binding affinity and kinetic analysis of targeted small molecule-modified nanoparticles. Bioconjug Chem. 21:14-19.
29. Timp W, Watson N, Sabban A, Zik O, Matsudaira P. 2006. Wet electron microscopy with quantum dots. BioTechniques. 41:295-298.
30. Tokumasu F, Dvorak J. 2003. Development and application of quantum dots for immunocytochemistry of human erythrocytes. J Microsc. 211:256-261.
31. Varikatt W, Yong JLC, Killingsworth MC. 2006. Somatostatinoma: collision with neurofibroma and ultrastructural features. Histol Histopathol. 21:1171-1180.
32. Vicidomini G, Gagliani MC, Canfora M, Cortese K, Frosi F, Santangelo C, Di Fiore PP, Boccacci P, Diaspro A, Tacchetti C. 2008. High data output and automated 3D correlative lightelectron microscopy method. Traffic. 9:1828-1838.
33. Yamashita S, Katsumata O, Okada Y. 2009. Establishment of a standard post-embedding method for immunoelectron microscopy by applying heat-induced antigen retrieval. J Electron Microsc. 58:267-279.
34. Yamazaki K, Eyden BP. 1998. Characterisation of breast stromal fibroblasts: cell surface distribution of collagen type IV, laminin and fibronectin. J Submicrosc Cytol Pathol. 30:217-226.
35. Yano S, Kashima K, Daa T, Urabe S, Tsuji K, Nakayama I, Yokoyama S. 2003. An antigen retrieval method using alkaline solution allows immunoelectron microscopic identification of secretory granules in conventional epoxy-embedded tissue sections. J Histochem Cytochem. 51:199-204.
36. Yi H, Leunissen J, Shi G, Gutekunst C, Hersch S. 2001. A novel procedure for pre-embedding double immunogold-silver labeling at the ultrastructural level. J Histochem Cytochem. 49:279-284.
37. Yong JL, Killingsworth MC, Spicer ST, Wu XJ. 2010. Case report. Fibronectin non-amyloid glomerulopathy. Int J Clin Exp Pathol. 3:210-216.