Establishment of a standardized post-embedding method for immunoelectron microscopy by applying heat-induced antigen retrieval

Journal of Electron Microscopy

Volumn 58, Issue 4, 2009, Pages 267-279

  Yamashita, Shuji ^a   Katsumata, Osamu ^b   Okada, Yasunori ^a  

^a KEIO UNIVERSITY   (Japan)

^b KITASATO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Electron staining; Heat induced antigen retrieval; Immunoelectron microscopy; Post embedding method; Tannic acid

Indexed keywords

ANTIGEN; TANNIN DERIVATIVE;

ANIMAL; ARTICLE; EVALUATION; FEMALE; HEATING; HISTOLOGY; IMMUNOELECTRON MICROSCOPY; IMMUNOLOGY; INTESTINE; KIDNEY; METHODOLOGY; MOUSE; PANCREAS; RABBIT; REPRODUCIBILITY; TISSUE FIXATION; ULTRASTRUCTURE; UTERUS;

ANIMALS; ANTIGENS; FEMALE; HEATING; INTESTINES; KIDNEY; MICE; MICROSCOPY, IMMUNOELECTRON; PANCREAS; RABBITS; REPRODUCIBILITY OF RESULTS; TANNINS; TISSUE EMBEDDING; TISSUE FIXATION; UTERUS;

ANTIGENS; FLAVONOIDS; LEAD COMPOUNDS; MAGNESIUM COMPOUNDS; TANNINS; URANIUM COMPOUNDS;

ACID BUFFERS; BUFFER PH; ELECTRON STAINING; EMBEDDING METHOD; HEAT-INDUCED ANTIGEN RETRIEVAL; IMMUNOELECTRON MICROSCOPY; PHOSPHATE BUFFERS; POST-EMBEDDING METHOD; STANDARDIZED METHODS; TANNIC ACID;

CHLORINE COMPOUNDS;

EID: 68349141486     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfp017     Document Type: Article

References (37)

1. Shi S R, Key M E, and Kaira K L (1991) Antigen retrieval in formalin- fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39: 741-748.
2. Cattoretti G, Pileri S, Parravicini C, Becker M H, Poggi S, Bifulco C, Key G, D'Amato L, Sabattini E, Feudale E, Reynold F, Gerdes J, and Rilke F (1993) Antigen unmasking on formalin-fixed, paraffin embedded tissue sections. J. Pathol. 171: 83-98.
3. Werner M, Von Wasielewski R, and Komminoth P (1996) Antigen retrieval, signal amplification and intensification in immunohisto chemistry. Histochem. Cell. Biol. 105: 253-260.
4. Pileri S A, Roncador G, Ceccarelli C, Piccioli M, Briskomatis A, Sabattini E, Ascani S, Santini D, Piccaluga P P, Leone O, Damiani S, Ercolessi C, Sandri F, Pieri F, Leoncini L, and Falini B (1997) Antigen retrieval techniques in immunohistochemistry: comparison of different methods. J. Pathol. 183: 116-123.
5. Shi S R, Cote R J, and Taylor C R (2001) Antigen retrieval techniques: current perspectives. J. Histochem. Cytochem. 49: 931-937.
6. Emoto K, Yamashita S, and Okada Y (2005) Mechanisms of heat- induced antigen retrieval: does pH or ionic strength of the solution play a role for refolding antigens? J. Histochem. Cytochem. 53: 1311-1321.
7. Yamashita S, and Okada Y (2005) Application of heat-induced antigen retrieval to aldehyde-fixed fresh frozen sections . J. Histochem. Cytochem. 53: 1421-1432.
8. Yamashita S, and Okada Y (2005) Mechanisms of heat-induced antigen retrieval: analyses in vitro employing SDS-PAGE and immuno- histochemistry J. Histochem. Cytochem. 53: 13-21
9. Kakimoto K, Takekoshi S, Miyajima K, and Osamura R Y (2008) Hypothesis for the mechanism for heat-induced antigen retrieval occurring on fresh frozen sections without formalin-fixation in immunohistochemistry. J. Mol. Histol. 39: 389-399.
10. Yamashita S (2007) Heat-induced antigen retrieval: mechanisms and application to histochemistry. Prog. Histochem. Cytochem. 41: 141-200.
11. Mayer G, and Bendayan M (2001) Amplification methods for the immunolocalization for rare molecules in cells and tissues. Prog. Histochem. Cytochem. 36: 8-85.
12. Rocken C, and Roessner A (1999) An evaluation of antigen retrieval procedures for immunoelectron microscopic classification of amyloid deposits. J. Histochem. Cytochem. 47: 1385-1394.
13. Brorson S H (2001) Heat-induced antigen retrieval of epoxy sections for electron microscopy. Histol. Histopathol. 16: 923-930.
14. Groos S, Reale E, and Lucianao L (2001) Re-evaluation of epoxy resin sections for light and electron microscopic immunostaining. J. Histochem. Cytochem. 49: 397-406.
15. Hann C R, Springett M J, and Johnson D H (2001) Antigen retrieval of basement membrane proteins from archival eye tissues. J. His- tochem. Cytochem. 49: 475-482.
16. Saito N, Konishi K, Takeda H, Kato M, Sugiyama T, and Asaka M (2003) Antigen retrieval trial for post-embedding immunoelec- tron microscopy by heating with several unmasking solutions. J. Histochem. Cytochem. 51: 989-994.
17. Yano S, Kashima K, Daa T, Urabe S, Tsuji K, Nakayama I, and Yokoyama S (2003) An antigen retrieval method using an alkaline solution allows immunoelectron microscopic identification of secretory granules in conventional epoxy-embedded tissue sections. J. Histochem. Cytochem. 51: 199-204.
18. Brorson S H (2004) A method for measurements of the efficiency of immunogold labeling of epoxy-embedded proteins subjected to different retrieval techniques. Micron 35: 619-621.
19. Goode NP, Shires M, CrellinDM, KhanTN, andMooneyAF (2004) Post-embedding double-labeling of antigen-retrieved ultrathin sections using a silver enhancement-controlled sequential immunogold (SECSI) technique. J. Histochem. Cytochem. 52: 141-144.
20. Fossmark R, Martinsen TC, Qvistad G, Bendheim M, Kopstad G, Kashima K, and Waldum H L (2005) Ultrastructure and chromo- granin A immunogold labeling for ECL cell carcinoids. APMIS. 113: 506-512.
21. Xiao J C, Adam A, Ruck P, and Kaiserling E (1996) A comparison of methods for heat-mediated antigen retrieval for immunoelectron microscopy: demonstration of cytokeratin no 18 in normal and neoplastic hepatocytes Biotech. Histochem. 71 : 278-285
22. Leong A S, and Sormunen R T (1998) Microwave procedures for electron microscopy and resin-embedded sections Micron 29: 397-409
23. Solberg L B, Melhus G, Broson S H, Wendel M, and Reinholt F P (2006) Heat-induced retrieval of immunogold labeling for nucleobindin and osteoadherin from Lowicryl sections of bone Micron 37: 347-.354
24. Slot J W, and Geuze H J (1985) A new method of preparing gold probes for multiple-labeling cytochemistry Eur. J. Cell. Biol. 38: 87-93
25. Berod A, Hartman B K, and Pujol J F (1981) Importance of fixation in immunohistochemistry: use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase J. Histochem. Cytochem. 2 9: 844-850.
26. Eldred W D, Zuker C, Karten H J, and Yazulla S (1983) Comparison of fixation and penetration enhancement techniques for use in ultrastructural immunocytochemistry J. Histochem. Cytochem. 31: 285-.292
27. Elliott E, Dennison C, Fortgens P H, and Travis J (1995) Paraformaldehyde fixation of neutrophils for immunolabeling of granule antigens in cryoultrasections J. Histochem. Cytochem. 43: 1019-1025
28. Baker G, and McCrea J (1966) The fine structure resulting from fixation with formaldehyde: the effects of concentration, duration and temperature J. R. Microsc. Soc. 85: 391-399
29. Maunsbach A B (1966) The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells: II Effects of varying osmolarity, ionic strength, buffer system and fixative concentration of glutaraldehyde solutions J. Ultrastruct.Re.s . 1 5 : 283-309
30. Bone Q, and Denton E J (1971) The osmotic effects of electron microscope fixatives J. Cell. Biol. 49: 571-581
31. Penttila A, McDonell E M, and Trump B F (1975) Effects of fixation and postfixation treatments on volume of injured cells J. Histochem. Cytochem. 23: 251-270.
32. Bullock G R (1983) The current status of fixation for electron microscopy: a review J. Microsc. 133: 1-15
33. Roth J, Taatjes D J, and Tokuyasu K T (1990) Contrasting of Lowicryl K4M thin sections. Histochemistry 95: 123-136.
34. Mizuhira V, and Futaesaku Y (1972) New fixation for biological membranes using tannic acids. Acta Histochem. Cytochem. 5: 233-236.
35. Hayat M A (1993) Stains and CytochemicalMethods (Plenum, New York and London).
36. Berryman M A, Porter WR, RodewaldRD, and Hubbard A L (1992) Effects of tannic acid on antigenicity and membrane contrast in ultrastructural immunocytochemistry. J. Histochem. Cytochem. 40: 845-857.
37. Phend K D, Rustioni A, and Weinberg R J (1995) An osmium-free method of epon embedment that preserves both ultrastructure and antigenicity for post-embedding immunocytochemistry . J. Histochem. Cytochem. 43: 283-292.