Improved immunogold labeling of epoxy sections by the use of propylene oxide as additional agent in dehydration, infiltration and embedding

Micron

Volumn 27, Issue 5, 1996, Pages 345-353

  Brorson, Sverre Henning ^a  

^a ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

dehydration; electron microscopy; embedding; Epoxy resin; immunocytochemistry; immunoelectron microscopy; immunogold; infiltration; LR White; propylene oxide

Indexed keywords

EID: 0030265486     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(96)00042-X     Document Type: Article

References (11)

1. Bowdler, A. L., Griffiths, D. F. R. and Newman, G. R., 1989. The morphological and immunohistochemical analysis of renal biopsies by light and electron microscopy using a processing method. Histochem. J., 21, 393-02.
2. Brorson, S. H. and Skjørten, F., 1996a. Improved technique for immunoelectron microscopy. How to prepare epoxy resin to obtain approximate the same immunogold labeling for epoxy sections as for acrylic sections without any etching. Micron, 27, 211-217.
3. Brorson, S. H. and Skjørten, F., 1996b. The theoretical relationship of immunogold labeling on acrylic sections and epoxy sections. Micron, 27, 193-201.
4. Brorson, S. H., Roos, N. and Skjørten, F., 1994. Antibody penetration into LR-White sections. Micron, 25, 453-460.
5. Causton, B. E., 1984. The choice of resins for electron immunocytochemistry. In: Immunolabeling for Electron Microscopy. Polak. J. M., Varndell, I. M. (eds). Elsevier Science Publishers, Amsterdam, pp. 29-36.
6. Hayat, M. A., 1989a. Rinsing, dehydration and embedding. In: Principles and Techniques of Electron Microscopy. The Macmillan Press Ltd, p. 88.
7. Hayat, M. A., 1989b. Rinsing, dehydration and embedding. In: Principles and Techniques of Electron Microscopy. The Macmillan Press Ltd. pp. 94-95.
8. Hayat, M. A., 1970. Polymerization. In: Principles and Techniques of Electron Microscopv Vol. 1. Van Nostrand Reinhold Company, p. 140.
9. Kellenberger, E., Dürrenberger, M., Villiger, W., Carlemalm, E. and Wurtz, M., 1987. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. J. Histochem. Cytochem., 35, 959-969.
10. Kellenberger, E., Villiger, W. and Carlemalm, E., 1986. The influence of the surface relief of thin sections of embedded, unstained biological material on image quality. Micron and Microscopica Acta, 17, 331-348.
11. Mark, H. F., Bikales, N. M., Overberger, C. G. and Menges, G., 1986. Encyclopaedia of Polymer Sciences and Engineering Vol. 6. Kroschwitz, J. I. (ed.). John Wiley and Sons, pp. 335-400.