Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots

Nature Methods

Volumn 2, Issue 10, 2005, Pages 743-749

  Giepmans, Ben N G ^a   Deerinck, Thomas J ^a   Smarr, Benjamin L ^a   Jones, Ying Z ^a   Ellisman, Mark H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONNEXIN 43; GLIAL FIBRILLARY ACIDIC PROTEIN; NEUROFILAMENT PROTEIN; NEUROFILAMENT PROTEIN 68; QUANTUM DOT; UNCLASSIFIED DRUG;

ACCURACY; ANIMAL CELL; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CORRELATION ANALYSIS; ELECTRON MICROSCOPY; FETUS; HIGH THROUGHPUT SCREENING; IMAGE ANALYSIS; IMAGING SYSTEM; MALE; MICROSCOPY; MOLECULAR SIZE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; RAT; SPECTRAL SENSITIVITY;

ANIMALS; CELLS, CULTURED; MICE; MICROSCOPY; MICROSCOPY, ELECTRON; PROTEINS; QUANTUM DOTS; RATS; TISSUE DISTRIBUTION;

EID: 27144475807     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth791     Document Type: Article

References (28)

1. Anderson, K.D., Karle, E.J. & Reiner, A. A pre-embedding triple-label electron microscopic immunohistochemical method as applied to the study of multiple inputs to defined tegmental neurons. J. Histochem. Cytochem. 42, 49-56 (1994).
2. Nisman, R., Dellaire, G., Ren, Y., Li, R. & Bazett-Jones, D.P. Application of quantum dots as probes for correlative fluorescence, conventional, and energy-filtered transmission electron microscopy. J. Histochem. Cytochem. 52, 13-18 (2004).
3. Takizawa, T., Suzuki, K. & Robinson, J.M. Correlative microscopy using FluoroNanogold on ultrathin cryosections. Proof of principle. J. Histochem. Cytochem. 46, 1097-1102 (1998).
4. Shiao, Y.H., Resau, J.H., Nagashima, K., Anderson, L.M. & Ramakrishna, G. The von Hippel-Lindau tumor suppressor targets to mitochondria. Cancer Res. 60, 2816-2819 (2000).
5. Deerinck, T.J. et al. Fluorescence photooxidation with eosin: A method for high resolution immunolocalization and in situ hybridization detection for light and electron microscopy. J. Cell Biol. 126, 901-910 (1994).
6. Gaietta, G. et al. Multicolor and electron microscopic imaging of connexin trafficking. Science 296, 503-507 (2002).
7. Chan, W.C. & Nie, S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281, 2016-2018 (1998).
8. Michalet, X. et al. Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307, 538-544 (2005).
9. Han, M., Gao, X., Su, J.Z. & Nie, S. Quantum dot-tagged microbeads for multiplexed optical coding of biomolecules. Nat. Biotechnol. 19 631-635 (2001).
10. Chan, W.C. et al. Luminescent quantum dots for multiplexed biological detection and imaging. Curr. Opin. Biotechnol. 13 40-46 (2002).
11. Voura, E.B., Jaiswal, J.K., Mattoussi, H. & Simon, S.M. Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy. Nat. Med. 10, 993-998 (2004).
12. Grecco, H.E. et al. Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells. Microsc Res. Tech. 65, 169-179 (2004).
13. Liu, C., Miller, P.D., Henstrom, W.L. & Gibson, J.M. Transmission electron microscopy of semiconductor quantum dots. J. Microsc. 199, 130-140 (2000).
14. Herve, J.C., Bourmeyster, N. & Sarrouilhe, D. Diversity in protein-protein interactions of connexins: Emerging roles. Biochim. Biophys. Acta 1662, 22-41 (2004).
15. Giepmans, B.N. Gap junctions and connexin-interacting proteins. Cardiovasc Res. 62, 233-245 (2004).
16. Humbel, B.M., de Jong, M.D., Muller, W.H. & Verkleij, A.J. Pre-embedding immunolabeling for electron microscopy: An evaluation of permeabilization methods and markers. Microsc. Res. Tech. 42 43-58 (1998).
17. Martone, M.E., Deerinck, T.J., Yamada, N., Bushong, E. & Ellisman, M.H. Correlated 3D light and electron microscopy: Use of high voltage electron microscopy and electron tomography for imaging large biological structures. J. Histotechnology 23, 261-270 (2000).
18. Phend, K.D., Rustioni, A. & Weinberg, R.J. An osmium-free method of epon embedment that preserves both ultrastructure and antigenicity for post-embedding immunocytochemistry. J. Histochem. Cytochem. 43 283-292 (1995).
19. Horisberger, M. & Vauthey, M. Labelling of colloidal gold with protein. A quantitative study using β-lactoglobulin. Histochemistry 80 13-18 (1984).
20. Mansson, A. et al. In vitro sliding of actin filaments labelled with single quantum dots. Biochem. Biophys. Res. Commun. 314 529-534 (2004).
21. Hanaki, K. et al. Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker. Biochem. Biophys. Res. Commun. 302, 496-501 (2003).
22. Akerman, M.E., Chan, W.C., Laakkonen, P., Bhatia, S.N. & Ruoslahti, E. Nanocrystal targeting in vivo. Proc. Natl. Acad. Sci. USA 99 12617-12621 (2002).
23. Gao, X., Cui, Y., Levenson, R.M., Chung, L.W. & Nie, S. In vivo cancer targeting and imaging with semiconductor quantum dots. Nat. Biotechnol. 22, 969-976 (2004).
24. Jain, R.K. & Stroh, M. Zooming in and out with quantum dots. Nat. Biotechnol. 22, 959-960 (2004).
25. Bobik, M., Ellisman, M.H., Rudy, B. & Martone, M.E. Potassium channel subunit Kv3.2 and the water channel aquaporin-4 are selectively localized to cerebellar pinceau. Brain Res. 1026, 168-178 (2004).
26. Lidke, D.S. et al. Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction. Nat. Biotechnol. 22, 198-203 (2004).
27. Zhang, J., Campbell, R.E., Ting, A.Y. & Tsien, R.Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3, 906-918 (2002).
28. Tour, O., Meijer, R.M., Zacharias, D.A., Adams, S.R. & Tsien, R.Y. Genetically targeted chromophore-assisted light inactivation. Nat. Biotechnol. 21, 1505-1508 (2003).