Re-evaluation of epoxy resin selection for light and electron microscopic immunostaining

Journal of Histochemistry and Cytochemistry

Volumn 49, Issue 3, 2001, Pages 397-406

  Groos, S ^a   Reale, E ^a   Luciano, L ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Adherens junction; Antigen retrieval; E cadherin; Etching; Immunogold labeling; catenin; catenin

Indexed keywords

ALCOHOL; ALPHA CATENIN; BETA CATENIN; EPOXY RESIN; PARAFFIN; SODIUM HYDROXIDE; UVOMORULIN;

ANTIGEN RETRIEVAL; ARTICLE; CELL JUNCTION; CELL MEMBRANE; CONTROLLED STUDY; ELECTRON MICROSCOPY; FROZEN SECTION; HEAT TREATMENT; HUMAN; HUMAN TISSUE; IMMUNOGOLD STAINING; IMMUNOHISTOCHEMISTRY; MICROSCOPY; MORPHOLOGY; PRIORITY JOURNAL; SMALL INTESTINE; TISSUE SECTION;

EID: 0035117959     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540104900313     Document Type: Article

References (48)

1. The combination of high-accelerator epoxy resin and antigen retrieval to obtain more intense immunolabeling on epoxy sections than on LR-White sections for large proteins
2. Comparison of the immunolabeling of heated and deplasticized epoxy sections on the electron microscopical level
3. Mechanism for antigen detection on deplasticized epoxy sections
4. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB-1 and MIB-3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections
5. The unmasking of antigens in paraffin sections of tissue by trypsin
6. Microwave superheating enhances immunocytochemistry in the freshly frozen rat brain
7. Novel E-cadherin-mediated adhesion in peripheral nerve: Schwann cell architecture is stabilized by autotypic adherens junctions
8. Junctional complexes in various epithelia
9. Reaction of formaldehyde with proteins. VI. Cross-linking of amino groups with phenol, imidazole, or indole groups
10. Interkeratinocyte adherens junctions: Immunocytochemical visualization of cell-cell junctional structures, distinct from desmosomes, in human epidermis
11. In vivo analysis of cadherin function in the mouse intestinal epithelium: Essential roles in adhesion, maintenance of differentiation, and regulation of programmed cell death
12. Forced expression of E-cadherin in the mouse intestinal epithelium slows cell migration and provides evidence for nonautomatous regulation of cell fate in a self-renewing system
13. Estimating the effect of etching agents on plastic sections
14. Altetation of β-catenin expression in colonic epithelial cells of familial adenomatous polyposis patients
15. The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: CDNA cloning and immunoelectron microscopy
16. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy
17. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions
18. Investigation of immunogold-silver staining by electron microscopy
19. Differential staining of ultrathin sections of epon embedded tissues for light microscopy
20. Ultrathin frozen sections. II. Demonstration of enzyme activity
21. Apc gene mutation is associated with a dominant-negative effect upon intestinal cell migration
22. Cor-relative light and electron microscopic immunocytochemistry on the same section with colloidal gold
23. Colloidal gold immunostaining on deplasticized ultra-thin sections
24. A simple method for removing the resin from epoxy-embedded tissue
25. The use of proteolytic enzymes to improve immunoglobulin staining by the PAP technique
26. Post-embedding electron microscopic immunocytochemistry
27. Subcellular localization of the APC protein: Immunoelectron microscopic study of the association of the APC protein with catenin
28. Adenohypophysis: Ultrastructural cytochemistry. A review
29. Dünndarm
30. Use and abuse of LR-White
31. Modern acrylics for post-embedding immunostaining techniques
32. Brief, high-temperature heat denaturation (pressure cooking): A simple and effective method of antigen retrieval for routinely processed tissues
33. An evaluation of antigen retrieval procedures for immunoelectron microscopic classification of amyloid deposits
34. Immunohistochemical localization of IgA antigens in sections embedded in epoxy resin
35. The tumor suppressor protein APC colocalizes with β-catenin in the colon epithelial cells
36. Antigen retrieval immunohistochemistry: Past, present and future
37. Antigen retrieval in formalinfixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections
38. Microwave-stimulated antigen retrieval for immunohistology and immunoelectron microscopy of resin-embedded sections
39. Antigen unmasking for immunoelectron microscopy: Labeling is improved by treating with sodium ethoxide or sodium metaperiodate, then heating on retrieval medium
40. β-catenin is frequently mutated and demonstrates altered cellular location in azoxymethane-induced rat colon tumors
41. An approach to posternbedding staining of protein (immunoglobulin) antigen embedded in plastic. Prerequisites and limitations
42. Immunohistochemistry on ultrathin frozen sections
43. Expression of E-cadherin-associated molecules (α- β- and γ-catenins and p120) in colorectal polyps
44. Antigen retrieval for electron microscopy using a microwave technique for epithelial and basal lamina antigens
45. Immunohistochemical localization of cell surface receptors using a novel method permitting simple, rapid and reliable LM/EM correlation
46. Molecular and functional analysis of cadherin-based adherens junctions