Characterization and evolution of a metagenome-derived lipase towards enhanced enzyme activity and thermostability

Molecular and Cellular Biochemistry

Volumn 373, Issue 1-2, 2013, Pages 149-159

  Kumar, Rakesh ^a   Sharma, Monika ^a   Singh, Ranvir ^a   Kaur, Jagdeep ^a  

^a PANJAB UNIVERSITY   (India)

Author keywords

Activity; Directed evolution; Lipase; Metagenomic; Thermostability

Indexed keywords

ENZYME VARIANT; LIPR1 PROTEIN; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; HYDROPHOBICITY; METAGENOME; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN INTERACTION; SEQUENCE HOMOLOGY; TEMPERATURE; THERMOSTABILITY; WILD TYPE;

AMINO ACID MOTIFS; BACTERIAL PROTEINS; CATALYTIC DOMAIN; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; LIPASE; METAGENOME; MODELS, MOLECULAR; SOIL MICROBIOLOGY; STRUCTURAL HOMOLOGY, PROTEIN;

EID: 84871776493     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-012-1483-8     Document Type: Article

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