Characterization of a thermostable lipase showing loss of secondary structure at ambient temperature

Molecular Biology Reports

Volumn 39, Issue 3, 2012, Pages 2795-2804

  Sharma, Pushpender Kumar ^a   Singh, Kashmir ^a   Singh, Ranvir ^a   Capalash, Neena ^a   Ali, Azmat ^b   Mohammad, Owais ^b   Kaur, Jagdeep ^a  

^a PANJAB UNIVERSITY   (India)

^b ALIGARH MUSLIM UNIVERSITY   (India)

Author keywords

Catalysis; Expression; Lipase; Metagenome; Spectroscopic

Indexed keywords

ACETONE; COPPER CHLORIDE; DNA; HEXANE; MERCURIC CHLORIDE; PHYSOSTIGMINE; RECOMBINANT ENZYME; TRIACYLGLYCEROL LIPASE; TRITON X 100;

ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; DNA EXTRACTION; DNA ISOLATION; ENVIRONMENTAL TEMPERATURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME CONFORMATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; GENE EXPRESSION; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; SOIL; TEMPERATURE SENSITIVITY; THERMOSTABILITY;

BASE SEQUENCE; CIRCULAR DICHROISM; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; DNA PRIMERS; HALF-LIFE; HOT SPRINGS; HYDROGEN-ION CONCENTRATION; INDIA; LIPASE; METAGENOMICS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; SEQUENCE ANALYSIS, DNA; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84863851532     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-011-1038-1     Document Type: Article

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