Catalysis uncoupling in a glutamine amidotransferase bienzyme by unblocking the glutaminase active site

Chemistry and Biology

Volumn 19, Issue 12, 2012, Pages 1589-1599

  List, Felix ^a,b   Vega, M Cristina ^a,c   Razeto, Adelia ^a   Häger, Michaela C ^b   Sterner, Reinhard ^b   Wilmanns, Matthias ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b UNIVERSITY OF REGENSBURG   (Germany)

^c CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDOPHOSPHORIBOSYLTRANSFERASE; AMMONIA; ENZYME; GLUTAMINE; IMIDAZOLEGLYCEROLPHOSPHATE SYNTHASE; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONFORMATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; MUTATION; NONHUMAN; PRIORITY JOURNAL; STEADY STATE;

AMINOHYDROLASES; AMMONIA; CATALYSIS; CATALYTIC DOMAIN; GLUTAMINASE; GLUTAMINE; LIGASES; MODELS, MOLECULAR; PROTEIN CONFORMATION; THERMOTOGA MARITIMA;

EID: 84871539111     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chembiol.2012.10.012     Document Type: Article

References (39)

1. R. Amaro, E. Tajkhorshid, and Z. Luthey-Schulten Developing an energy landscape for the novel function of a (beta/alpha)8 barrel: ammonia conduction through HisF Proc. Natl. Acad. Sci. USA 100 2003 7599 7604
2. R.E. Amaro, R.S. Myers, V.J. Davisson, and Z.A. Luthey-Schulten Structural elements in IGP synthase exclude water to optimize ammonia transfer Biophys. J. 89 2005 475 487
3. R.E. Amaro, A. Sethi, R.S. Myers, V.J. Davisson, and Z.A. Luthey-Schulten A network of conserved interactions regulates the allosteric signal in a glutamine amidotransferase Biochemistry 46 2007 2156 2173
4. S. Beismann-Driemeyer, and R. Sterner Imidazole glycerol phosphate synthase from Thermotoga maritima. Quaternary structure, steady-state kinetics, and reaction mechanism of the bienzyme complex J. Biol. Chem. 276 2001 20387 20396
5. Collaborative Computational Project, Number 4 The CCP4 suite: programs for protein crystallography Acta Crystallogr. D Biol. Crystallogr. 50 1994 760 763
6. B.N. Chaudhuri, S.C. Lange, R.S. Myers, S.V. Chittur, V.J. Davisson, and J.L. Smith Crystal structure of imidazole glycerol phosphate synthase: a tunnel through a (beta/alpha)8 barrel joins two active sites Structure 9 2001 987 997
7. B.N. Chaudhuri, S.C. Lange, R.S. Myers, V.J. Davisson, and J.L. Smith Toward understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerolphosphate synthase: crystal structures of a ternary complex and the free enzyme Biochemistry 42 2003 7003 7012
8. V.J. Davisson, I.L. Deras, S.E. Hamilton, and L.L. Moore A plasmid-based approach for the synthesis of a histidine biosynthetic intermediate J. Org. Chem. 58 1994 137 143
9. A. Douangamath, M. Walker, S. Beismann-Driemeyer, M.C. Vega-Fernandez, R. Sterner, and M. Wilmanns Structural evidence for ammonia tunneling across the (beta alpha)(8) barrel of the imidazole glycerol phosphate synthase bienzyme complex Structure 10 2002 185 193
10. E. Frieden, and C. Walter Prevalence and significance of the product inhibition of enzymes Nature 198 1963 834 837
11. M. Goto, R. Omi, N. Nakagawa, I. Miyahara, and K. Hirotsu Crystal structures of CTP synthetase reveal ATP, UTP, and glutamine binding sites Structure 12 2004 1413 1423
12. G. Guédez, K. Hipp, V. Windeisen, B. Derrer, M. Gengenbacher, B. Böttcher, I. Sinning, B. Kappes, and I. Tews Assembly of the eukaryotic PLP-synthase complex from Plasmodium and activation of the Pdx1 enzyme Structure 20 2012 172 184
13. S.N. Ho, H.D. Hunt, R.M. Horton, J.K. Pullen, and L.R. Pease Site-directed mutagenesis by overlap extension using the polymerase chain reaction Gene 77 1989 51 59
14. R.M. Horton, H.D. Hunt, S.N. Ho, J.K. Pullen, and L.R. Pease Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension Gene 77 1989 61 68
15. T.J. Klem, and V.J. Davisson Imidazole glycerol phosphate synthase: the glutamine amidotransferase in histidine biosynthesis Biochemistry 32 1993 5177 5186
16. E. Krissinel, and K. Henrick Secondary-structure matching (SSM), a new tool for fast protein structure alignment in three dimensions Acta Crystallogr. D Biol. Crystallogr. 60 2004 2256 2268
17. B. Lee, and F.M. Richards The interpretation of protein structures: estimation of static accessibility J. Mol. Biol. 55 1971 379 400
18. C. Liebold, F. List, H.R. Kalbitzer, R. Sterner, and E. Brunner The interaction of ammonia and xenon with the imidazole glycerol phosphate synthase from Thermotoga maritima as detected by NMR spectroscopy Protein Sci. 19 2010 1774 1782
19. J.M. Lipchock, and J.P. Loria Nanometer propagation of millisecond motions in V-type allostery Structure 18 2010 1596 1607
20. F. List, M. Bocola, M.C. Haeger, and R. Sterner Constitutively active glutaminase variants provide insights into the activation mechanism of anthranilate synthase Biochemistry 51 2012 2812 2818
21. L.J. Messenger, and H. Zalkin Glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli. Purification and properties J. Biol. Chem. 254 1979 3382 3392
22. H.A. Milman, D.A. Cooney, and C.Y. Huang Studies on the mechanism of the glutamine-dependent reaction catalyzed by asparagine synthetase from mouse pancreas J. Biol. Chem. 255 1980 1862 1866
23. S. Mouilleron, and B. Golinelli-Pimpaneau Conformational changes in ammonia-channeling glutamine amidotransferases Curr. Opin. Struct. Biol. 17 2007 653 664
24. S. Mouilleron, M.A. Badet-Denisot, B. Badet, and B. Golinelli-Pimpaneau Dynamics of glucosamine-6-phosphate synthase catalysis Arch. Biochem. Biophys. 505 2011 1 12
25. R.S. Myers, J.R. Jensen, I.L. Deras, J.L. Smith, and V.J. Davisson Substrate-induced changes in the ammonia channel for imidazole glycerol phosphate synthase Biochemistry 42 2003 7013 7022
26. R.S. Myers, R.E. Amaro, Z.A. Luthey-Schulten, and V.J. Davisson Reaction coupling through interdomain contacts in imidazole glycerol phosphate synthase Biochemistry 44 2005 11974 11985
27. D.L. Ollis, E. Cheah, M. Cygler, B. Dijkstra, F. Frolow, S.M. Franken, M. Harel, S.J. Remington, I. Silman, and J. Schrag The alpha/beta hydrolase fold Protein Eng. 5 1992 197 211
28. R. Omi, H. Mizuguchi, M. Goto, I. Miyahara, H. Hayashi, H. Kagamiyama, and K. Hirotsu Structure of imidazole glycerol phosphate synthase from Thermus thermophilus HB8: open-closed conformational change and ammonia tunneling J. Biochem. 132 2002 759 765
29. F.M. Raushel, P.M. Anderson, and J.J. Villafranca Kinetic mechanism of Escherichia coli carbamoyl-phosphate synthetase Biochemistry 17 1978 5587 5591
30. F.M. Raushel, J.B. Thoden, and H.M. Holden Enzymes with molecular tunnels Acc. Chem. Res. 36 2003 539 548
31. I. Rivalta, M.M. Sultan, N.S. Lee, G.A. Manley, J.P. Loria, and V.S. Batista Allosteric pathways in imidazole glycerol phosphate synthase Proc. Natl. Acad. Sci. USA 109 2012 E1428 E1436
32. F.B. Rudolph Product inhibition and abortive complex formation Methods Enzymol. 63 1979 411 436
33. W.P. Schneider, B.P. Nichols, and C. Yanofsky Procedure for production of hybrid genes and proteins and its use in assessing significance of amino acid differences in homologous tryptophan synthetase alpha polypeptides Proc. Natl. Acad. Sci. USA 78 1981 2169 2173
34. M. Strohmeier, T. Raschle, J. Mazurkiewicz, K. Rippe, I. Sinning, T.B. Fitzpatrick, and I. Tews Structure of a bacterial pyridoxal 5′-phosphate synthase complex Proc. Natl. Acad. Sci. USA 103 2006 19284 19289
35. D. Stüber, H. Matile, and G. Garotta System for High-Level Production in Escherichia coli and Rapid Purification of Recombinant Proteins: Application to Epitope Mapping, Preparation of Antibodies, and Structure-Function Analysis 1990 Academic Press Orlando, FL
36. J.B. Thoden, S.G. Miran, J.C. Phillips, A.J. Howard, F.M. Raushel, and H.M. Holden Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis Biochemistry 37 1998 8825 8831
37. J.B. Thoden, X. Huang, F.M. Raushel, and H.M. Holden The small subunit of carbamoyl phosphate synthetase: snapshots along the reaction pathway Biochemistry 38 1999 16158 16166
38. R. Thoma, G. Obmolova, D.A. Lang, M. Schwander, P. Jenö, R. Sterner, and M. Wilmanns Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis FEBS Lett. 454 1999 1 6
39. H. Zalkin, and J.L. Smith Enzymes utilizing glutamine as an amide donor Adv. Enzymol. Relat. Areas Mol. Biol. 72 1998 87 144