Two protein lysine methyltransferases methylate outer membrane protein B from Rickettsia

Journal of Bacteriology

Volumn 194, Issue 23, 2012, Pages 6410-6418

  Abeykoon, Amila H ^a   Chao, Chien Chung ^b   Wang, Guanghui ^c   Gucek, Marjan ^c   Yang, David C H ^a   Ching, Wei Mei ^b  

^a GEORGETOWN UNIVERSITY   (United States)

^b NAVAL MEDICAL RESEARCH CENTER   (United States)

^c NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; BACTERIAL PROTEIN; GENOMIC DNA; LYSINE; LYSINE METHYLTRANSFERASE METHYLATE; METHYL [[3 METHYL 2 [[5 METHYL 3 (2 METHYLALLYL) 4 OXO 2 THIAZOLIDINYLIDENE]HYDRAZONO] 4 OXO 5 THIAZOLIDINYL]] PHOSPHATE SODIUM; METHYLTRANSFERASE; NICKEL; NITRILOTRIACETIC ACID; OUTER MEMBRANE PROTEIN B; RECOMBINANT PROTEIN; S ADENOSYLMETHIONINE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOINFORMATICS; CATALYSIS; CLONING; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; LIQUID CHROMATOGRAPHY; METHYLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; RADIOACTIVITY; RICKETTSIA; TANDEM MASS SPECTROMETRY; WESTERN BLOTTING;

BACTERIAL OUTER MEMBRANE PROTEINS; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; HISTONE-LYSINE N-METHYLTRANSFERASE; ISOTOPE LABELING; KINETICS; RECOMBINANT PROTEINS; RICKETTSIA PROWAZEKII; S-ADENOSYLMETHIONINE; TANDEM MASS SPECTROMETRY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; RICKETTSIA; RICKETTSIA PROWAZEKII;

EID: 84871050281     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.01379-12     Document Type: Article

References (39)

1. Anacker RL, Philip RN, Williams JC, List RH, Mann RE. 1984. Biochemical and immunochemical analysis of Rickettsia rickettsii strains of various degrees of virulence. Infect. Immun. 44:559-564.
2. Andersson SGE, et al. 1998. The genome sequence of Rickettsia prowazekii and the origin of mitochondria. Nature 396:133-140.
3. Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK. 2007. Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Nat. Struct. Mol. Biol. 14:1214-1220.
4. Bechah Y, et al. 2010. Genomic, proteomic, and transcriptomic analysis of virulent and avirulent Rickettsia prowazekii reveals its adaptive mutation capabilities. Genome Res. 20:655-663.
5. Blanc G, et al. 2005. Molecular evolution of Rickettsia surface antigens: evidence of positive selection. Mol. Biol. Evol. 22:2073-2083.
6. Bourgeois AL, Dasch GA. 1981. The species specific surface protein antigens of Rickettsia typhi: immunogenicity and protective efficiency in guinea pig, p 71-80. In Burgdorfer W, Anacker RL (ed), Rickettsiae and rickettsial diseases. Academic Press, New York, NY.
7. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
8. Chan YG, Cardwell MM, Hermanas TM, Uchiyama T, Martinez JJ. 2009. Rickettsial outer-membrane protein B (rOmpB) mediates bacterial invasion through Ku70 in an actin, c-Cbl, clathrin and caveolin 2-dependent manner. Cell Microbiol. 11:629-644.
9. Chan YG, Riley SP, Chen E, Martinez JJ. 2011. Molecular basis of immunity to rickettsial infection conferred through outer membrane protein B. Infect. Immun. 79:2303-2313.
10. Chao CC, Zhang Z, Wang H, Alkhalil A, Ching WM. 2008. Serological reactivity and biochemical characterization of methylated and unmethylated forms of a recombinant protein fragment derived from outer membrane protein B of Rickettsia typhi. Clin. Vaccine Immunol. 15:684-690.
11. Ching WM, Wang H, Davis J, Dasch GA. 1993. Amino acid analysis and multiple methylation of lysine residues in the surface protein antigen of Rickettsia prowazekii, p 307-314, Techniques in protein chemistry, vol IV. Academic Press, New York, NY.
12. Dasch GA, Bourgeois AL. 1981. Antigens of typhus group of rickettsia: importance of the species-specific surface protein antigens in eliciting immunity, p 61-70. In Burgdorfer W, Anacker RL (ed), Rickettsiae and rickettsial diseases. Academic Press, New York, NY.
13. Dillon SC, Zhang X, Trievel RC, Cheng X. 2005. The SET-domain protein superfamily: protein lysine methyltransferases. Genome Biol. 6:227. doi:10.1186/gb-2005-6-8-227.
14. Dirk LMA, et al. 2007. Kinetic manifestation of processivity during multiple methylations catalyzed by SET domain protein methyltransferases. Biochemistry 46:3905-3915.
15. Feng HM, Whitworth T, Olano JP, Popov VL, Walker DH. 2004. Fc-dependent polyclonal antibodies and antibodies to outer membrane proteinsAand B, but not to lipopolysaccharide, protect SCID mice against fatal Rickettsia conorii infection. Infect. Immun. 72:2222-2228.
16. Feng HM, Whitworth T, Popov V, Walker DH. 2004. Effect of antibody on the rickettsia-host cell interaction. Infect. Immun. 72:3524-3530.
17. Govaerts C, Wille H, Prusiner SB, Cohen FE. 2004. Evidence for assembly of prions with left-handed β-helices into trimers. Proc. Natl. Acad. Sci. U. S. A. 101:8342-8347.
18. Huang J, Berger SL. 2008. The emerging field of dynamic lysine methylation of non-histone proteins. Curr. Opin. Genet. Dev. 18:152-158.
19. Jones DT. 1999. Protein secondary structure prediction based on position- specific scoring matrices. J. Mol. Biol. 292:195-202.
20. Kelley LA, Sternberg MJ. 2009. Protein structure prediction on the Web: a case study using the Phyre server. Nat. Protoc. 4:363-371.
21. Kinter M, Sherman NE. 2000. Protein sequencing and identification using tandem mass spectroscopy. Wiley Interscience, Hoboken, NJ.
22. Krishnan R, Lindquist SL. 2005. Structural insights into a yeast prion illuminate nucleation and strain diversity. Nature 435:765-772.
23. Li H, Walker DH. 1998. rOmpA is a critical protein for the adhesion of Rickettsia rickettsii to host cells. Microb. Pathog. 24:289-298.
24. Martinez JJ, Seveau S, Veiga E, Matsuyama S, Cossart P. 2005. Ku70, a component of DNA-dependent protein kinase, is a mammalian receptor for Rickettsia conorii. Cell 123:1013-1023.
25. Nelson R, et al. 2005. Structure of the cross-β spine of amyloid-like fibrils. Nature 435:773-778.
26. Raetz CRH, Roderick SL. 1995. A left-handed parallel β helix in the structure of UDP-N-acetylglucosamine acyltransferase. Science 270:997-1000.
27. Rea S, et al. 2000. Regulation of chromatin structure by site-specific histone H3 methyltransferases. Nature 406:593-599.
28. Rodionov AV. 1990. The nature of the post-translational modification of the common species-specific outer membrane protein of Rickettsia prowazekii. Bioorg. Khim. 16:1687-1688.
29. Rodionov AV, Eremeeva ME, Balaeva NM. 1991. Isolation and partial characterization of the Mr 100 K protein from Rickettsia prowazekii strains with different genetic relation and virulence, p 95-101. In Kazr J, RaoultD (ed), Rickettsiae and rickettsial diseases. Publishing House of The Slovak Academy of Sciences, Bratislava, Slovakia.
30. Rodionov AV, Eremeeva ME, Balayeva NM. 1991. Isolation and partial characterization of the M® 100 kD protein from Rickettsia prowazekii strains of different virulence. Acta Virol. 35:557-565.
31. Roux V, Raoult D. 2000. Phylogenetic analysis of members of the genus Rickettsia using the gene encoding the outer-membrane protein rOmpB (ompB). Int. J. Syst. Evol. Microbiol. 50(Pt 4):1449-1455.
32. Sawaya MR, et al. 2007. Atomic structures of amyloid cross-β spines reveal varied steric zippers. Nature 447:453-457.
33. Schulz GE. 2002. The structure of bacterial outer membrane proteins. Biochim. Biophys. Acta 1565:308-317.
34. Soding J, Biegert A, Lupas AN. 2005. The HHpred interactive server for protein homology detection and structure prediction. Nucleic Acids Res. 33:W244-W248.
35. Springer WR. 1991. A method for quantifying radioactivity associated with protein in silver-stained polyacrylamide gels. Anal. Biochem. 195: 172-176.
36. Uchiyama T, Kawano H, Kusuhara Y. 2006. The major outer membrane protein rOmpB of spotted fever group rickettsiae functions in the rickettsial adherence to and invasion of Vero cells. Microbes Infect. 8:801-809.
37. Wass MN, Kelley LA, Sternberg MJ. 2010. 3DLigandSite: predicting ligand-binding sites using similar structures. Nucleic Acids Res. 38(Suppl 2):W469-W473.
38. Xiao B, et al. 2003. Structure and catalytic mechanism of the human histone methyltransferase SET7/9. Nature 421:652-656.
39. Zhang J-Z, Hao J-F, Walker DH, Yu X-J. 2006. A mutation inactivating the methyltransferase gene in avirulent Madrid E strain of Rickettsia prowazekii reverted to wild type in the virulent revertant strain Evir. Vaccine 24:2317-2323.