메뉴 건너뛰기




Volumn 3, Issue 5, 2012, Pages 898-905

Advances in multiplex PCR: Balancing primer efficiencies and improving detection success

Author keywords

Diagnostic PCR; PCR optimisation; Primer sensitivity; Standardise DNA templates; Trophic interactions

Indexed keywords


EID: 84870934222     PISSN: None     EISSN: 2041210X     Source Type: Journal    
DOI: 10.1111/j.2041-210X.2012.00215.x     Document Type: Article
Times cited : (159)

References (45)
  • 3
    • 33646270475 scopus 로고    scopus 로고
    • PCR primer design
    • 2nd edn (eds C.W. Dieffenbach & G.S. Dveksler). Cold Spring Harbor Laboratory Press, Cold Spring Harbor
    • Apte, A. & Daniel, S. (2003) PCR primer design. PCR Primer: A Laboratory Manual, 2nd edn (eds C.W. Dieffenbach & G.S. Dveksler), pp. 61-74. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
    • (2003) PCR Primer: A Laboratory Manual , pp. 61-74
    • Apte, A.1    Daniel, S.2
  • 5
    • 79955979786 scopus 로고    scopus 로고
    • Which prey sustains cold-adapted invertebrate generalist predators in arable land? Examining prey choices by molecular gut-content analysis
    • Eitzinger, B. & Traugott, M. (2011) Which prey sustains cold-adapted invertebrate generalist predators in arable land? Examining prey choices by molecular gut-content analysis. Journal of Applied Ecology, 48, 591-599.
    • (2011) Journal of Applied Ecology , vol.48 , pp. 591-599
    • Eitzinger, B.1    Traugott, M.2
  • 7
    • 79955850880 scopus 로고    scopus 로고
    • Correlation of maple sap composition with bacterial and fungal communities determined by multiplex automated ribosomal intergenic spacer analysis (MARISA)
    • Filteau, M., Lagace, L., LaPointe, G. & Roy, D. (2011) Correlation of maple sap composition with bacterial and fungal communities determined by multiplex automated ribosomal intergenic spacer analysis (MARISA). Food Microbiology, 28, 980-989.
    • (2011) Food Microbiology , vol.28 , pp. 980-989
    • Filteau, M.1    Lagace, L.2    LaPointe, G.3    Roy, D.4
  • 8
    • 0028528103 scopus 로고
    • DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates
    • Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294-299.
    • (1994) Molecular Marine Biology and Biotechnology , vol.3 , pp. 294-299
    • Folmer, O.1    Black, M.2    Hoeh, W.3    Lutz, R.4    Vrijenhoek, R.5
  • 9
    • 77955318529 scopus 로고    scopus 로고
    • Highly sensitive multiplex PCR for simultaneous detection and discrimination of Dirofilaria immitis and Dirofilaria repens in canine peripheral blood
    • Gioia, G., Lecova, L., Genchi, M., Ferri, E., Genchi, C. & Mortarino, M. (2010) Highly sensitive multiplex PCR for simultaneous detection and discrimination of Dirofilaria immitis and Dirofilaria repens in canine peripheral blood. Veterinary Parasitology, 172, 160-163.
    • (2010) Veterinary Parasitology , vol.172 , pp. 160-163
    • Gioia, G.1    Lecova, L.2    Genchi, M.3    Ferri, E.4    Genchi, C.5    Mortarino, M.6
  • 10
    • 79751507750 scopus 로고    scopus 로고
    • Unnecessary roughness? Testing the hypothesis that predators destined for molecular gut-content analysis must be hand-collected to avoid cross-contamination
    • Greenstone, M.H., Weber, D.C., Coudron, T.C. & Payton, M.E. (2011) Unnecessary roughness? Testing the hypothesis that predators destined for molecular gut-content analysis must be hand-collected to avoid cross-contamination. Molecular Ecology Resources, 11, 286-293.
    • (2011) Molecular Ecology Resources , vol.11 , pp. 286-293
    • Greenstone, M.H.1    Weber, D.C.2    Coudron, T.C.3    Payton, M.E.4
  • 12
    • 0002051540 scopus 로고    scopus 로고
    • BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT
    • Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
    • (1999) Nucleic Acids Symposium Series , vol.41 , pp. 95-98
    • Hall, T.A.1
  • 13
    • 77956187626 scopus 로고    scopus 로고
    • A multiplex PCR assay to diagnose and quantify Nosema infections in honey bees (Apis mellifera)
    • Hamiduzzaman, M.M., Guzman-Novoa, E. & Goodwin, P.H. (2010) A multiplex PCR assay to diagnose and quantify Nosema infections in honey bees (Apis mellifera). Journal of Invertebrate Pathology, 105, 151-155.
    • (2010) Journal of Invertebrate Pathology , vol.105 , pp. 151-155
    • Hamiduzzaman, M.M.1    Guzman-Novoa, E.2    Goodwin, P.H.3
  • 17
    • 69649089885 scopus 로고    scopus 로고
    • A 26plex autosomal STR assay to aid human identity testing
    • Hill, C.R., Butler, J.M. & Vallone, P.M. (2009) A 26plex autosomal STR assay to aid human identity testing. Journal of Forensic Sciences, 54, 1008-1015.
    • (2009) Journal of Forensic Sciences , vol.54 , pp. 1008-1015
    • Hill, C.R.1    Butler, J.M.2    Vallone, P.M.3
  • 18
    • 0036274502 scopus 로고    scopus 로고
    • Primary community assembly on land - the missing stages: why are the heterotrophic organisms always the first?
    • Hodkinson, I.D., Webb, N.R. & Coulson, S.J. (2002) Primary community assembly on land - the missing stages: why are the heterotrophic organisms always the first? Journal of Ecology, 90, 569-577.
    • (2002) Journal of Ecology , vol.90 , pp. 569-577
    • Hodkinson, I.D.1    Webb, N.R.2    Coulson, S.J.3
  • 19
    • 33845397629 scopus 로고    scopus 로고
    • Molecular identification of wolf spiders (Araneae: Lycosidae) by multiplex polymerase chain reaction
    • Hosseini, R., Keller, M.A., Schmidt, O. & Framenau, V.W. (2007) Molecular identification of wolf spiders (Araneae: Lycosidae) by multiplex polymerase chain reaction. Biological Control, 40, 128-135.
    • (2007) Biological Control , vol.40 , pp. 128-135
    • Hosseini, R.1    Keller, M.A.2    Schmidt, O.3    Framenau, V.W.4
  • 20
    • 34548505059 scopus 로고    scopus 로고
    • A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples
    • Kanuch, P., Hajkova, P., Rehak, Z. & Bryja, J. (2007) A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples. Acta Chiropterologica, 9, 277-282.
    • (2007) Acta Chiropterologica , vol.9 , pp. 277-282
    • Kanuch, P.1    Hajkova, P.2    Rehak, Z.3    Bryja, J.4
  • 21
    • 0034882364 scopus 로고    scopus 로고
    • Invertebrate succession on an Alpine glacier foreland
    • Kaufmann, R. (2001) Invertebrate succession on an Alpine glacier foreland. Ecology, 82, 2261-2278.
    • (2001) Ecology , vol.82 , pp. 2261-2278
    • Kaufmann, R.1
  • 22
    • 38849100922 scopus 로고    scopus 로고
    • Molecular analysis of predation: a review of best practice for DNA-based approaches
    • King, R.A., Read, D.S., Traugott, M. & Symondson, W.O.C. (2008) Molecular analysis of predation: a review of best practice for DNA-based approaches. Molecular Ecology, 17, 947-963.
    • (2008) Molecular Ecology , vol.17 , pp. 947-963
    • King, R.A.1    Read, D.S.2    Traugott, M.3    Symondson, W.O.C.4
  • 23
    • 77950240195 scopus 로고    scopus 로고
    • Prey choice by carabid beetles feeding on an earthworm community analysed using species- and lineage-specific PCR primers
    • King, R.A., Vaughan, I.P., Bell, J.R., Bohan, D.A. & Symondson, W.O.C. (2010) Prey choice by carabid beetles feeding on an earthworm community analysed using species- and lineage-specific PCR primers. Molecular Ecology, 19, 1721-1732.
    • (2010) Molecular Ecology , vol.19 , pp. 1721-1732
    • King, R.A.1    Vaughan, I.P.2    Bell, J.R.3    Bohan, D.A.4    Symondson, W.O.C.5
  • 25
    • 0025606714 scopus 로고
    • Chromosomal localization and copy number of 18S+28S ribosomal-RNA genes in evolutionarily diverse mosquitos (Diptera, Culicidae)
    • Kumar, A. & Rai, K.S. (1990) Chromosomal localization and copy number of 18S+28S ribosomal-RNA genes in evolutionarily diverse mosquitos (Diptera, Culicidae). Hereditas, 113, 277-289.
    • (1990) Hereditas , vol.113 , pp. 277-289
    • Kumar, A.1    Rai, K.S.2
  • 26
    • 43949105816 scopus 로고    scopus 로고
    • Group-specific primers for DNA-based detection of springtails (Hexapoda: Collembola) within predator gut contents
    • Kuusk, A.K. & Agustí, N. (2008) Group-specific primers for DNA-based detection of springtails (Hexapoda: Collembola) within predator gut contents. Molecular Ecology Resources, 8, 678-681.
    • (2008) Molecular Ecology Resources , vol.8 , pp. 678-681
    • Kuusk, A.K.1    Agustí, N.2
  • 27
    • 41849098577 scopus 로고    scopus 로고
    • Application of the multiplex PCR method for discrimination of Artemisia iwayomogi from other Artemisia herbs
    • Lee, M.Y., Doh, E.J., Kim, E.S., Kim, Y.W., Ko, L.S. & Oh, S.E. (2008) Application of the multiplex PCR method for discrimination of Artemisia iwayomogi from other Artemisia herbs. Biological & Pharmaceutical Bulletin, 31, 685-690.
    • (2008) Biological & Pharmaceutical Bulletin , vol.31 , pp. 685-690
    • Lee, M.Y.1    Doh, E.J.2    Kim, E.S.3    Kim, Y.W.4    Ko, L.S.5    Oh, S.E.6
  • 28
    • 7044281587 scopus 로고    scopus 로고
    • Ribosomal DNA gene and phylogenetic relationships of Diplura and lower Hexapods
    • Luan, Y., Zhang, Y., Yue, Q., Pang, J., Xie, R. & Yin, W. (2003) Ribosomal DNA gene and phylogenetic relationships of Diplura and lower Hexapods. Science in China (Series C), 46, 67-76.
    • (2003) Science in China (Series C) , vol.46 , pp. 67-76
    • Luan, Y.1    Zhang, Y.2    Yue, Q.3    Pang, J.4    Xie, R.5    Yin, W.6
  • 32
    • 65249134960 scopus 로고    scopus 로고
    • Identification of the shark species Rhizoprionodon lalandii and R. porosus (Elasmobranchii, Carcharhinidae) by multiplex PCR and PCR-RFLP techniques
    • Mendonca, F.F., Hashimoto, D.T., Porto-Foresti, F., Oliveira, C., Gadig, O.B.F. & Foresti, F. (2009) Identification of the shark species Rhizoprionodon lalandii and R. porosus (Elasmobranchii, Carcharhinidae) by multiplex PCR and PCR-RFLP techniques. Molecular Ecology Resources, 9, 771-773.
    • (2009) Molecular Ecology Resources , vol.9 , pp. 771-773
    • Mendonca, F.F.1    Hashimoto, D.T.2    Porto-Foresti, F.3    Oliveira, C.4    Gadig, O.B.F.5    Foresti, F.6
  • 33
    • 79551640733 scopus 로고    scopus 로고
    • Host blood meal identification by multiplex polymerase chain reaction for dispersal evidence of stable flies (Diptera: Muscidae) between livestock facilities
    • Pitzer, J.B., Kaufman, P.E., Tenbroeck, S.H. & Maruniak, J.E. (2011) Host blood meal identification by multiplex polymerase chain reaction for dispersal evidence of stable flies (Diptera: Muscidae) between livestock facilities. Journal of Medical Entomology, 48, 53-60.
    • (2011) Journal of Medical Entomology , vol.48 , pp. 53-60
    • Pitzer, J.B.1    Kaufman, P.E.2    Tenbroeck, S.H.3    Maruniak, J.E.4
  • 35
    • 67649220893 scopus 로고    scopus 로고
    • Multiplex PCR-based simultaneous amplification of selectable marker and reporter genes for the screening of genetically modified crops
    • Randhawa, G.J., Chhabra, R. & Singh, M. (2009) Multiplex PCR-based simultaneous amplification of selectable marker and reporter genes for the screening of genetically modified crops. Journal of Agricultural and Food Chemistry, 57, 5167-5172.
    • (2009) Journal of Agricultural and Food Chemistry , vol.57 , pp. 5167-5172
    • Randhawa, G.J.1    Chhabra, R.2    Singh, M.3
  • 36
    • 78149386916 scopus 로고    scopus 로고
    • Real-time and multiplex real-time polymerase chain reactions for the detection of Bartonella henselae within cat flea, Ctenocephalides felis, samples
    • Robinson, M.T., Morgan, E.R., Woods, D. & Shaw, S.E. (2010) Real-time and multiplex real-time polymerase chain reactions for the detection of Bartonella henselae within cat flea, Ctenocephalides felis, samples. Medical and Veterinary Entomology, 24, 449-455.
    • (2010) Medical and Veterinary Entomology , vol.24 , pp. 449-455
    • Robinson, M.T.1    Morgan, E.R.2    Woods, D.3    Shaw, S.E.4
  • 38
    • 0028952289 scopus 로고
    • Optimization and troubleshooting in PCR
    • Roux, K.H. (1995) Optimization and troubleshooting in PCR. PCR Methods and Applications, 4, S185-S194.
    • (1995) PCR Methods and Applications , vol.4
    • Roux, K.H.1
  • 40
    • 34247098019 scopus 로고    scopus 로고
    • Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targeting bacterial community analysis
    • Sipos, R., Szekely, A.J., Palatinszky, M., Revesz, S., Marialigeti, K. & Nikolausz, M. (2007) Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targeting bacterial community analysis. Fems Microbiology Ecology, 60, 341-350.
    • (2007) Fems Microbiology Ecology , vol.60 , pp. 341-350
    • Sipos, R.1    Szekely, A.J.2    Palatinszky, M.3    Revesz, S.4    Marialigeti, K.5    Nikolausz, M.6
  • 44
    • 84858119238 scopus 로고    scopus 로고
    • Generalist predators disrupt parasitoid aphid control by direct and coincidental intraguild predation
    • Traugott, M., Bell, J.R., Raso, L., Sint, D. & Symondson, W.O.C. (2012) Generalist predators disrupt parasitoid aphid control by direct and coincidental intraguild predation. Bulletin of Entomological Research, 102, 239-247.
    • (2012) Bulletin of Entomological Research , vol.102 , pp. 239-247
    • Traugott, M.1    Bell, J.R.2    Raso, L.3    Sint, D.4    Symondson, W.O.C.5
  • 45
    • 77949589618 scopus 로고    scopus 로고
    • Rapid diagnostic multiplex PCR (RD-PCR) to discriminate Schistosoma haematobium and S. bovis
    • Webster, B.L., Rollinson, D., Stothard, J.R. & Huyse, T. (2010) Rapid diagnostic multiplex PCR (RD-PCR) to discriminate Schistosoma haematobium and S. bovis. Journal of Helminthology, 84, 107-114.
    • (2010) Journal of Helminthology , vol.84 , pp. 107-114
    • Webster, B.L.1    Rollinson, D.2    Stothard, J.R.3    Huyse, T.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.