Highly sensitive multiplex PCR for simultaneous detection and discrimination of Dirofilaria immitis and Dirofilaria repens in canine peripheral blood

Veterinary Parasitology

Volumn 172, Issue 1-2, 2010, Pages 160-163

  Gioia, G ^a   Lecova L ^b   Genchi, M ^c   Ferri, E ^d   Genchi, C ^a   Mortarino, M ^a  

^a UNIVERSITY OF MILAN   (Italy)

^b UNIVERSITY OF VETERINARY MEDICINE   (Slovakia)

^c UNIVERSITY OF PAVIA   (Italy)

^d UNIVERSITY OF MILANO BICOCCA   (Italy)

Author keywords

Blood; Dirofilaria immitis; Dirofilaria repens; Dog; Microfilariae; Multiplex PCR

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE;

AMPLICON; ARTICLE; CONTROLLED STUDY; DIROFILARIA IMMITIS; DIROFILARIA REPENS; DNA EXTRACTION; GENE AMPLIFICATION; MITOCHONDRION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; PHENOTYPE; RELIABILITY; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENTIATION;

ANIMALS; DIROFILARIA IMMITIS; DIROFILARIASIS; DNA, HELMINTH; DOG DISEASES; DOGS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

CANIS FAMILIARIS; DIROFILARIA; DIROFILARIA IMMITIS; DIROFILARIA REPENS; NEMATODA;

EID: 77955318529     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2010.04.027     Document Type: Article

References (15)

1. Bandi C., Damiani G., Magrassi L., Gigolo A., Fani R., Sacchi L. Flavobacteria as intracellular symbionts in cockroaches. Proc. R. Soc. Lond. B 1994, 257:43-48.
2. Casiraghi M., Bain O., Guerriero R., Martin C., Pocacqua V., Gardner S.L., Franceschi A., Bandi C. Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution. Int. J. Parasitol. 2004, 34:191-203.
3. Casiraghi M., Bazzocchi C., Mortarino M., Ottina E., Genchi C. A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris). Vet. Parasitol. 2006, 141:368-372.
4. Courtney C.H., Zeng Q.Y., MacKinnon B.R. Comparison of two antigen tests and the modified Knott's test for detection of canine heartworm at different worm burdens. Canine Pract. 1993, 18:5-7.
5. Favia G., Lanfrancotti A., Della Torre A., Cancrini G., Coluzzi M. Polymerase chain reaction identification of Dirofilaria repens and Dirofilaria immitis. Parasitology 1996, 113:567-571.
6. Genchi C., Venco L., Genchi M. Guideline for the laboratory diagnosis of canine and feline Dirofilaria infections. Mappe Parassitologiche 8 2007, 137-144. Rolando Editore, Naples. C. Genchi, L. Rinaldi, G. Cringoli (Eds.).
7. Genchi C., Rinaldi L., Mortarino M., Genchi M., Cringoli G. Climate and Dirofilaria infection in Europe. Vet. Parasitol. 2009, 163:286-292.
8. Lachaud L., Marchergui-Hammami S., Chabbert E., Dereure J., Dedet J.P., Bastien P. Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. J. Clin. Microbiol. 2002, 40:210-215.
9. Mar P.H., Yang I.C., Chang G.N., Fei A.C. Specific polymerase chain reaction for differential diagnosis of Dirofilaria immitis and Dipetalonema reconditum using primers derived from internal transcribed spacer region 2 (ITS2). Vet. Parasitol. 2002, 106:243-252.
10. McCall J.W., Genchi C., Kramer L.H., Guerrero J., Venco L. Heartworm disease in animals and humans. Adv. Parasitol. 2008, 66:193-285.
11. Mishra K., Raj D.K., Hazra R.K., Dash A.P., Supakar P.C. The development and evaluation of a single step multiplex PCR method for simultaneous detection of Brugia malayi and Wuchereria bancrofti. Mol. Cell. Probes 2007, 21:355-362.
12. Mortarino M., Gelain M.E., Gioia G., Ciusani E., Bazzocchi C., Comazzi S. ZAP-70 and Syk expression in canine lymphoid cells and preliminary results on leukaemia cases. Vet. Immunol. Immunopathol. 2009, 128:395-401.
13. Nuchprayoon S., Junpee A., Poovorawan Y., Scott A.L. Detection and differentiation of filarial parasites by universal primers and polymerase chain reaction-restriction fragment length polymorphism analysis. Am. J. Trop. Med. Hyg. 2005, 73:895-900.
14. Rishniw M., Barr S.C., Simpson K.W., Frongillo M.F., Franz M., Alpizar J.L.D. Discrimination between six species of canine microfilariae by a single polymerase chain reaction. Vet. Parasitol. 2006, 135:305-314.
15. Simón F., Morchón R., González-Miguel J., Marcos-Atxutegi C., Siles-Lucas M. What is new about animal and human dirofilariosis?. Trends Parasitol. 2009, 25:404-409.