Unnecessary roughness? Testing the hypothesis that predators destined for molecular gut-content analysis must be hand-collected to avoid cross-contamination

Molecular Ecology Resources

Volumn 11, Issue 2, 2011, Pages 286-293

  Greenstone, Matthew H ^a   Weber, Donald C ^a   Coudron, Thomas C ^b   Payton, Mark E ^c  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

^c OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

Molecular gut content analysis; Sampling

Indexed keywords

ANIMAL; ARTICLE; BEETLE; CHEMISTRY; DNA CONTAMINATION; EVALUATION; GENETIC PROCEDURES; GENETICS; HETEROPTERA; METHODOLOGY; PHYSIOLOGY; PREDATION; STOMACH JUICE; ZOOLOGY;

ANIMALS; BEETLES; DNA CONTAMINATION; ENTOMOLOGY; GASTROINTESTINAL CONTENTS; GENETIC TECHNIQUES; HETEROPTERA; PREDATORY BEHAVIOR;

ANIMALIA; ARTHROPODA; COLEOMEGILLA MACULATA; LEPTINOTARSA; LEPTINOTARSA DECEMLINEATA; LEPTINOTARSA JUNCTA; PODISUS MACULIVENTRIS; SOLANUM TUBEROSUM;

EID: 79751507750     PISSN: 1755098X     EISSN: 17550998     Source Type: Journal    
DOI: 10.1111/j.1755-0998.2010.02922.x     Document Type: Article

References (42)

1. Agustí N, de Vicente MC, Gabarra R (2000) Developing SCAR markers to study predation on Trailurodes vaporareorum. Insect Molecular Biology, 9, 263-268.
2. Benton AH, Crump J (1981) Observations on the spring and summer behavior of the 12-spotted ladybird beetle, Coleomegilla maculata (DeGeer) (Coleoptera: Coccinellidae). Journal of the New York Entomological Society, 89, 102-105.
3. Chapman EG, Romero SA, Harwood JD (2010) Maximizing collection and minimizing risk: does vacuum suction sampling increase the likelihood for misinterpretation of food web connections? Molecular Ecology Resources. Doi.
4. Chen Y, Giles KL, Payton ME, Greenstone MH (2000) Identifying key cereal aphid predators by molecular gut analysis. Molecular Ecology, 9, 1887-1898.
5. Clark MS, Luna JM, Stone ND, Youngman RR (1993) Habitat preferences of generalist predators in reduced-tillage corn. Journal of Entomological Science, 28, 404-416.
6. Coudron TA, Wittmeyer JL, Kim Y (2002) Life history and cost analysis for continuous rearing of Podisus maculiventris (Say) (Heteroptera: Pentatomidae) on a zoophytophagous diet. Journal of Economic Entomology, 95, 1159-1168.
7. Cuthbertson GS, Fleming CC, Kurchie AK (2003) Detection of Rhopalosiphum insertum (apple-grass aphid) by the predatory mite Anystis baccarum using molecular gut analysis. Agricultural and Forest Entomology, 5, 219-225.
8. Fournier V, Hagler JR, Daane KM, de Leon JH, Groves RL (2008) Identifying the predator complex of Homalodisca vitripennis (Hemiptera: Cicadellidae): a comparative study of an ELISA and PCR gut content assay. Oecologia, 157, 629-640.
9. Greenstone MH, Rowley DL, Heimbach U, Lundgren JG, Pfannenstiel RS, Rehner SA (2005) Barcoding generalist predators by polymerase chain reaction: carabids and spiders. Molecular Ecology, 14, 3247-3266.
10. Greenstone MH, Rowley DR, Weber DC, Hawthorne DJ (2007) Feeding mode and prey detectability half-lives in molecular gut-content analysis: an example with two predators of the Colorado potato beetle. Bulletin of Entomological Research, 97, 201-209.
11. Greenstone MH, Szendrei Z, Rowley DL, Payton ME, Weber DC (2010) Choosing natural enemies for conservation biological control: use of the prey detectability half-life to rank key predators of Colorado potato beetle. Entomologia Experimentalis et Applicata, 137, 97-107.
12. Hagler JR, Naranjo SE (1994) A qualitative survey of two coleopteran predators of Bemisia tabaci (Homoptera: Alyerodidae) and Pectinophora gossypiella (Lepidoptera: Gelechiidae) using a multiple prey gut content ELISA. Environmental Entomology, 23, 193-197.
13. Hagler JR, Naranjo SE (2005) Use of a gut content ELISA to detect whitefly predator feeding activity after field exposure to different insecticide treatments. Biocontrol Science and Technology, 15, 321-339.
14. Hagler JR, Cohen AC, Bradley-Dunlop D, Enriquez FJ (1992) Field evaluation of predation on Lygus hesperus (Hemiptera: Miridae) using a species- and stage-specific monoclonal antibody. Environmental Entomology, 21, 896-900.
15. Harwood JD (2008) Are sweep net sampling and pitfall trapping compatible with molecular analysis of predation? Environmental Entomology, 37, 990-995.
16. Harwood JD, Greenstone MH (2008) Molecular diagnosis of natural enemy-host interactions. In: Recent Advances in Insect Physiology, Toxicology and Molecular Biology (ed. Liu N), pp. 41-57. Research Signpost, Kerala, India.
17. Harwood JD, Sunderland KD, Symondson WOC (2004) Prey selection by linyphiid spiders: molecular tracking of the effects of alternative prey on rates of aphid consumption in the field. Molecular Ecology, 13, 3549-3560.
18. Harwood JD, Desneux N, Yoo HJS et al. (2007) Tracking the role of alternative prey in soybean aphid predation by Orius insidiosus: a molecular approach. Molecular Ecology, 16, 4390-4400.
19. Hazzard RV, Ferro DN, van Driesche RG, Tuttle AF (1991) Mortality of eggs of the Colorado potato beetle (Coleoptera: Chrysomelidae) from predation by Coleomegilla maculata (Coleoptera: Coccinellidae). Environmental Entomology, 20, 841-848.
20. Heimpel GE, Hough-Goldstein JA (1992) A survey of arthropod predators of Leptinotarsa decemlineata (Say) in Delaware potato fields. Journal of Agricultural Entomology, 9, 137-142.
21. Hemenway R, Whitcomb WH (1967) Ground beetles of the genus Lebia Latreille in Arkansas (Coleoptera: Carabidae): ecology and geographical distribution. Arkansas Academy of Science Proceedings, 21, 15-20.
22. Hilbeck A, Eckel C, Kennedy GG (1997) Predation on Colorado potato beetle eggs by generalist predators in research and commercial potato plantings. Biological Control, 8, 191-196.
23. Juen A, Traugott M (2007) Revealing species-specific trophic links in soil food webs: molecular identification of scarab predators. Molecular Ecology, 16, 1545-1557.
24. Kemp BM, Smith DG (2005) Use of bleach to eliminate contaminating DNA from the surface of bones and teeth. Forensic Science International, 154, 53-61.
25. King RA, Read DS, Traugott M, Symondson WOC (2008) Molecular analysis of predation: a review of best practice for DNA-based approaches. Molecular Ecology, 17, 947-963.
26. Kuusk AK, Cassel-Lundhagen A, Kvarnheden A, Ekbom B (2008) Tracking aphid predation by lycosid spiders in spring-sown cereals using PCR-based gut-content analysis. Basic and Applied Ecology, 9, 718-725.
27. Linville JG, Wells JD (2002) Surface sterilization of a maggot using bleach does not interfere with mitochondrial DNA analysis of crop contents. Journal of Forensic Sciences, 47, 1055-1059.
28. Lundgren JG, Ellsbury ME, Prischmann DA (2009) Analysis of the predator community of a subterranean herbivorous insect based on polymerase chain reaction. Ecological Applications, 19, 2157-2166.
29. Mack TP, Smilowitz Z (1980) The development of a green peach aphid natural enemy sampling procedure. Environmental Entomology, 9, 440-445.
30. McCauley DE (1992) Family structured patterns of mortality in the false Colorado potato beetle. Ecological Entomology, 17, 142-148.
31. Moir ML, Brennan KEC, Majer JD, Koch JM, Fletcher MJ (2005) An optimal sampling protocol for Hemiptera on understorey plants. Journal of Insect Conservation, 9, 3-20.
32. Prince AM, Andrus L (1992) PCR: How to kill unwanted DNA. BioTechniques, 12, 358-360.
33. Read DS, Sheppard SK, Bruford MW, Glen DM, Symondson WOC (2006) Molecular detection of predation by soil micro-arthropods on nematodes. Molecular Ecology, 15, 1963-1972.
34. Ruberson JR, Greenstone MH (1998) Predators of budworm/bollworm eggs in cotton: an immunological study. Proceedings of the 1998 Beltwide Cotton Production and Research Conference, 2, 1095-1098.
35. Sheppard SK, Henneman ML, Memmott J, Symondson WOC (2004) Infiltration by alien predators into invertebrate food webs in Hawaii: a molecular approach. Molecular Ecology, 13, 2077-2088.
36. Sigsgaard L, Greenstone MH, Duffield SJ (2002) Egg cannibalism in Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) on sorghum and pigeonpea. BioControl, 47, 151-165.
37. Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651-701.
38. Symondson WOC (2002) Molecular identification of prey in predator diets. Molecular Ecology, 11, 627-641.
39. Szendrei Z, Greenstone MH, Payton ME, Weber DC (2010) Molecular gut-content analysis of a predator assemblage reveals the effect of habitat manipulation on biological control in the field. Basic and Applied Ecology, 11, 153-161.
40. Weber DC, Lundgren JG (2009) Detection of predation using qPCR: effect of prey quantity, elapsed time, chaser diet, and sample preservation on detectable quantity of prey DNA. Journal of Insect Science, 9, 41. Available online.
41. Weber DC, Rowley DL, Greenstone MH, Athanas MM (2006) Prey preference and prey suitability of the predatory and parasitic carabid beetle, Lebia grandis, for several species of Leptinotarsa beetles. Journal of Insect Science, 6, 1-14. Available online.
42. Zaidi RH, Jaal Z, Hawkes NJ, Hemingway J, Symondson WOC (1999) Can multiple copy sequences of prey DNA be detected amongst the gut contents of invertebrate predators? Molecular Ecology, 8, 2081-2088.