Optimizing methods for PCR-based analysis of predation

Molecular Ecology Resources

Volumn 11, Issue 5, 2011, Pages 795-801

  Sint, Daniela ^a   Raso, Lorna ^a   Kaufmann, Rüdiger ^a   Traugott, Michael ^a  

^a UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

Annealing temperature; Molecular gut content analysis; Oreonebria; Pardosa; Replicability; Visualization methods

Indexed keywords

DNA; PRIMER DNA;

ANIMAL; ARTICLE; BEETLE; DIGESTION; DNA SEQUENCE; EVALUATION; FEEDING BEHAVIOR; FOOD CHAIN; GENETICS; GRYLLIDAE; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPIDER; TEMPERATURE;

ANIMALS; BASE SEQUENCE; BEETLES; DIGESTION; DNA; DNA PRIMERS; FEEDING BEHAVIOR; FOOD CHAIN; GRYLLIDAE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SPIDERS; TEMPERATURE;

ARANEAE; CARABIDAE; COLEOPTERA; LYCOSIDAE; OREONEBRIA; PARDOSA;

EID: 80051701364     PISSN: 1755098X     EISSN: 17550998     Source Type: Journal    
DOI: 10.1111/j.1755-0998.2011.03018.x     Document Type: Article

References (26)

1. von Berg K, Traugott M, Symondson WOC, Scheu S (2008) The effects of temperature on detection of prey DNA in two species of carabid beetle. Bulletin of Entomological Research, 98, 263-269.
2. Chapman EG, Romero SA, Harwood JD (2010) Maximizing collection and minimizing risk: does vacuum suction sampling increase the likelihood for misinterpretation of food web connections? Molecular Ecology Resources, 10, 1023-1033.
3. Deagle BE, Eveson JP, Jarman SN (2006) Quantification of damage in DNA recovered from highly degraded samples-a case study on DNA in faeces. Frontiers in Zoology, 3, 11.
4. Gariepy TD, Kuhlmann U, Gillott C, Erlandson M (2007) Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods. Journal of Applied Entomology, 131, 225-240.
5. Greenstone MH, Rowley DL, Weber DC, Payton ME, Hawthorne DJ (2007) Feeding mode and prey detectability half-lives in molecular gut-content analysis: an example with two predators of the Colorado potato beetle. Bulletin of Entomological Research, 97, 201-209.
6. Greenstone MH, Weber DC, Coudron TC, Payton ME (2011) Unnecessary roughness? Testing the hypothesis that predators destined for molecular gut-content analysis must be hand-collected to avoid cross-contamination. Molecular Ecology Resources, 11, 286-293.
7. Harper GL, King RA, Dodd CS et al. (2005) Rapid screening of invertebrate predators for multiple prey DNA targets. Molecular Ecology, 14, 819-827.
8. Harwood JD (2008) Are sweep net sampling and pitfall trapping compatible with molecular analysis of predation? Environmental Entomology, 37, 990-995.
9. Harwood JD, Sunderland KD, Symondson WOC (2004) Prey selection by linyphiid spiders: molecular tracking of the effects of alternative prey on rates of aphid consumption in the field. Molecular Ecology, 13, 3549-3560.
10. Harwood JD, Sunderland KD, Symondson WOC (2005) Monoclonal antibodies reveal the potential of the tetragnathid spider Pachygnatha degeeri (Araneae: Tetragnathidae) as an aphid predator. Bulletin of Entomological Research, 95, 161-167.
11. Hoogendoorn M, Heimpel GE (2001) PCR-based gut content analysis of insect predators: using ribosomal ITS-1 fragments from prey to estimate predation frequency. Molecular Ecology, 10, 2059-2067.
12. Hosseini R, Schmidt O, Keller MA (2008) Factors affecting detectability of prey DNA in the gut contents of invertebrate predators: a polymerase chain reaction-based method. Entomologia Experimentalis et Applicata, 126, 194-202.
13. Juen A, Traugott M (2005) Detecting predation and scavenging by DNA gut-content analysis: a case study using a soil insect predator-prey system. Oecologia, 142, 344-352.
14. King RA, Read DS, Traugott M, Symondson WOC (2008) Molecular analysis of predation: a review of best practice for DNA-based approaches. Molecular Ecology, 17, 947-963.
15. Kuusk A-K, Ekbom B (2010) Lycosid spiders and alternative food: feeding behavior and implications for biological control. Biological Control, 55, 20-26.
16. Ma J, Li D, Keller M, Schmidt O, Feng X (2005) A DNA marker to identify predation of Plutella xylostella (Lep., Plutellidae) by Nabis kinbergii (Hem., Nabidae) and Lycosa sp. (Aranaea, Lycosidae). Journal of Applied Entomology, 129, 330-335.
17. McMillan S, Kuusk A-K, Cassel-Lundhagen A, Ekbom B (2007) The influence of time and temperature on molecular gut content analysis: Adalia bipunctata fed with Rhopalosiphum padi. Insect Science, 14, 353-358.
18. Oehm J, Juen A, Nagiller K, Neuhauser S, Traugott M (2011) Molecular scatology: how to improve prey DNA detection success in avian faeces? Molecular Ecology Resources, doi:.
19. Piggott MP (2004) Effect of sample age and season of collection on reliability of microsatellite genotyping of faecal DNA. Wildlife Research, 31, 485-493.
20. Remén C, Krüger M, Cassel-Lundhagen A (2010) Successful analysis of gut contents in fungal-feeding oribatid mites by combining body-surface washing and PCR. Soil Biology and Biochemistry, 42, 1952-1957.
21. Seeber J, Rief A, Seeber GUH, Meyer E, Traugott M (2010) Molecular identification of detritivorous soil invertebrates from their faecal pellets. Soil Biology and Biochemistry, 42, 1263-1267.
22. Sheppard SK, Harwood JD (2005) Advances in molecular ecology: tracking trophic links through predator-prey food-webs. Functional Ecology, 19, 751-762.
23. Sheppard SK, Bell J, Sunderland KD et al. (2005) Detection of secondary predation by PCR analyses of the gut contents of invertebrate generalist predators. Molecular Ecology, 14, 4461-4468.
24. Symondson WOC (2002) Molecular identification of prey in predator diets. Molecular Ecology, 11, 627-641.
25. Traugott M, Symondson WOC (2008) Molecular analysis of predation on parasitized hosts. Bulletin of Entomological Research, 98, 223-231.
26. Weber DC, Lundgren JG (2009) Detection of predation using qPCR: effect of prey quantity, elapsed time, chaser diet, and sample preservation on detectable quantity of prey DNA. Journal of Insect Science, 9, 41. available online: insectscience.org/9.41.