A multiplex PCR assay to diagnose and quantify Nosema infections in honey bees (Apis mellifera)

Journal of Invertebrate Pathology

Volumn 105, Issue 2, 2010, Pages 151-155

  Hamiduzzaman, Mollah Md ^a   Guzman Novoa, Ernesto ^a   Goodwin, Paul H ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Identification; Infection levels; Nosema apis; Nosema ceranae; PCR; Spore

Indexed keywords

BACTERIAL DNA;

ANIMAL; ANIMAL DISEASE; ARTICLE; BEE; CLASSIFICATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MICROSPORIDIOSIS; NOSEMA; POLYMERASE CHAIN REACTION;

ANIMALS; BEES; DNA, BACTERIAL; MICROSPORIDIOSIS; NOSEMA; POLYMERASE CHAIN REACTION;

APIS MELLIFERA; APOIDEA; BACTERIA (MICROORGANISMS); MICROSPORIDIA; NOSEMA; NOSEMA APIS; NOSEMA CERANAE;

EID: 77956187626     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2010.06.001     Document Type: Article

References (16)

1. Bailey L., Ball B.V. Honey Bee Pathology 1991, Academic Press, London. second ed.
2. Bourgeois A.L., Rinderer T.E., Beaman L.D., Danka R.G. Genetic detection and quantification of Nosema apis and N. Ceranae in the honey bee. J. Invertebr. Pathol. 2010, 103:53-58.
3. Chen Y., Evans J.D., Smith I.B., Pettis J.S. Nosema ceranae is a long-present and wide-spread microsporidian infection of the European honey bee (Apis mellifera) in the United States. J. Invertebr. Pathol. 2008, 97:186-188.
4. De Graaf D.C., Masschelein G., Vandergeynst F., De Brabander H.F., Jacobs F.J. In vitro germination of Nosema apis (microspora: nosematidae) spores and its effect on their trehalose/D-glucose ratio. J. Invertebr. Pathol. 1993, 62:220-225.
5. Dean J.D., Goodwin P.H., Hsiang T. Comparison of relative RT-PCR and Northern blot analyses to measure expression of ß-1,3-glucanase in Nicotiana benthamiana infected with Colletotrichum destructivum. Plant Mol. Biol. Report. 2002, 20:347-356.
6. Evans, J.D., Wheeler, D.E., 2000. Expression profiles during honeybee caste determination. Genome Biol. 2:1, research 0001.1-0001.6.
7. Fries I., Martin R., Meana A., Garcia-Palencia P., Higes M. Natural infections of Nosema ceranae in European honey bees. J. Apic. Res. 2006, 45:230-233.
8. Higes M., Martin-Hernandez R., Meana A. Nosema ceranae, a new microsporidian parasite in honeybees in Europe. J. Invertebr. Pathol. 2006, 92:81-83.
9. Higes M., García P., Martín-Hernandez R., Biotas C., Bailon E.G., Gonzalez-Porto A.V., Barrios L., Del Nozal M.J., Bernal J.L., Jimenez J.J., Palencia P.G., Meana A. How natural infection by Nosema ceranae causes honeybee colony collapse. Environ. Microbiol. 2008, 10:2659-2669.
10. Hunt G.J. Insect DNA extraction protocol. Fingerprinting Methods Based on Arbitrarily Primed PCR 1997, 21-24. Springer-Verlag, Berlin. M.R. Micheli, R. Bova (Eds.).
11. Klee J., Besana A.M., Genersch E., Gisder S., Nanetti A., Tam D.Q., Chinh T.X., Puerta F., Ruz J.M., Kryger P., Message D., Hatjina F., Korpela S., Fries I., Paxton R.J. Widespread dispersal of the microsporidian Nosema ceranae, an emergent pathogen of the western honey bee, Apis mellifera. J. Invertebr. Pathol. 2007, 96:1-10.
12. Malone L.A., Gatehouse H.S., Tregidga E.L. Effects of time, temperature, and honey on Nosema apis (Microsporidia: Nosematidae), a parasite of the honeybee, Apis mellifera (Hymenoptera: Apidae). J. Invertebr. Pathol. 2001, 77:258-268.
13. Martin-Hernández R., Meana A., Prieto L., Salvador A.M., Garrido-bailon E., Higes M. Outcome of colonization of Apis mellifera by Nosema ceranae. Appl. Environ. Microbiol. 2007, 73:6331-6338.
14. Shimanuki, H., Knox, D.A., 2000. Diagnosis of Honey Bee Diseases, US Department of Agriculture, Agriculture Handbook No. AH-690, pp. 61.
15. Stankus T. A review and bibliography of the literature of honey bee colony collapse disorder: a poorly understood epidemic that clearly threatens the successful pollination of billions of dollars of crops in America. J. Agric. Food Inform. 2008, 9:115-143.
16. Thompson G.J., Yockey H., Lim J., Oldroyd B.P. Experimental manipulation of ovary activation and gene expression in honey bee (Apis mellifera) queens and workers: testing hypotheses of reproductive regulation. J. Exp. Zool. 2007, 307A:600-610.