Evidence for a dual functional role of a conserved histidine in RNA·DNA heteroduplex cleavage by human RNase H1

FEBS Journal

Volumn 279, Issue 24, 2012, Pages 4492-4500

  Alla, Nageswara R ^a   Nicholson, Allen W ^a  

^a Temple University   (United States)

Author keywords

antisense oligonucleotides; conserved histidine function; metal dependent phosphodiester hydrolysis; ribonuclease H1; RNA DNA heteroduplex

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; ALANINE; ANTISENSE OLIGONUCLEOTIDE; COBALT; DNA RNA HETERODUPLEX; HETERODUPLEX; HISTIDINE; MAGNESIUM ION; NICKEL; POTASSIUM CHLORIDE; RECOMBINANT ENZYME; RECOMBINANT RIBONUCLEASE H1; UNCLASSIFIED DRUG; ZINC ION;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA CLEAVAGE; ENZYME PURIFICATION; HYDROLYSIS; IN VITRO STUDY; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA CLEAVAGE; STOICHIOMETRY;

CATALYSIS; CATIONS, DIVALENT; DNA; HISTIDINE; HUMANS; HYDROGEN-ION CONCENTRATION; NUCLEIC ACID HETERODUPLEXES; PROTEOLYSIS; RECOMBINANT PROTEINS; RIBONUCLEASE H; RNA;

BACTERIA (MICROORGANISMS);

EID: 84870514532     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12035     Document Type: Article

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