High-yield production, refolding and a molecular modelling of the catalytic module of (1,3)-β-D-glucan (curdlan) synthase from Agrobacterium sp.

Glycoconjugate Journal

Volumn 27, Issue 4, 2010, Pages 461-476

  Hrmova, Maria ^a   Stone, Bruce A ^b   Fincher, Geoffrey B ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

^b LA TROBE UNIVERSITY   (Australia)

Author keywords

Escherichia coli; Family GT2 transferases; Heterologous expression; Homology model; Integral membrane proteins; pET 32a(+) and champion pET SUMO vectors

Indexed keywords

1,3 BETA GLUCAN SYNTHASE; ASPARTIC ACID; BETAINE; COMPLEMENTARY DNA; CURDLAN; CURDLAN SYNTHASE; DNA HYBRID; HYBRID PROTEIN; PLASMID DNA; PROTEINASE; RECOMBINANT PROTEIN; SULFOBETAINE; SUMO PROTEIN; THIOREDOXIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CIRCULAR DICHROISM; CONTROLLED STUDY; DNA VECTOR; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; ENZYME SYNTHESIS; ESCHERICHIA COLI; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN MOTIF; PROTEIN PURIFICATION; RHIZOBIUM;

AMINO ACID MOTIFS; ANIMALS; CATALYTIC DOMAIN; ESCHERICHIA COLI; GLUCOSYLTRANSFERASES; MODELS, MOLECULAR; PROTEIN REFOLDING; PROTEIN STRUCTURE, TERTIARY; RHIZOBIUM;

AGROBACTERIUM SP.; ESCHERICHIA COLI;

EID: 77955661339     PISSN: 02820080     EISSN: 15734986     Source Type: Journal    
DOI: 10.1007/s10719-010-9291-4     Document Type: Article

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