High yield purification of JNK1β1 and activation by in vitro reconstitution of the MEKK1 → MKK4 → JNK MAPK phosphorylation cascade

Protein Expression and Purification

Volumn 87, Issue 2, 2013, Pages 87-99

  Owen, Gavin R ^a   Achilonu, Ikechukwu ^a   Dirr, Heini W ^a  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

Author keywords

c Jun N terminal kinase; Codon harmonization; MAP kinases; MAPK signaling cascade; Protein phosphorylation

Indexed keywords

ACTIVATING TRANSCRIPTION FACTOR 2; ATF2 PROTEIN, HUMAN; MAP3K1 PROTEIN, HUMAN; MAP3K4 PROTEIN, HUMAN; MITOGEN ACTIVATED PROTEIN KINASE KINASE KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE KINASE KINASE 4; STRESS ACTIVATED PROTEIN KINASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; CODON; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PHOSPHORYLATION; SIGNAL TRANSDUCTION;

ACTIVATING TRANSCRIPTION FACTOR 2; CHROMATOGRAPHY, AFFINITY; CODON; ESCHERICHIA COLI; HUMANS; JNK MITOGEN-ACTIVATED PROTEIN KINASES; MAP KINASE KINASE KINASE 1; MAP KINASE KINASE KINASE 4; MAP KINASE SIGNALING SYSTEM; MODELS, MOLECULAR; PHOSPHORYLATION;

MLCS; MLOWN;

EID: 84870225621     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.10.010     Document Type: Article

References (39)

1. Chang, L., Mammalian MAP kinase signaling cascades. Nature 410 (2001), 37–40.
2. Chen, Z., Gibson, T.B., Robinson, F., Silvestro, L., Pearson, G., Xu, B., Wright, A., Vanderbilt, C., Cobb, M.H., MAP kinases. Chem. Rev. 101 (2001), 2449–2476.
3. Dérijard, B., Hibi, M., Wu, I.H., Barrett, T., Su, B., Deng, T., Karin, M., Davis, R.J., JNK1, a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell 76 (1994), 1025–1037.
4. Kyriakis, J.M., Avruch, J., Protein kinase cascades activated by stress and inflammatory cytokines. BioEssays 18 (1996), 567–577.
5. Pulverer, B.J., Kyriakis, J.M., Avruch, J., Nikolakaki, E., Wood-gett, J.R., Phosphorylation of c-jun mediated by MAP kinases. Nature 353 (1991), 670–674.
6. Davis, R.J., Signal transduction by the JNK group of MAP kinases. Cell, 103, 2000.
7. Ip, Y.T., Davis, R.J., Signal transduction by the c-Jun N-terminal kinase transduction by the c-Jun N-terminal kinase (JNK)–from inflammation to development. Curr. Opin. Cell Biol. 10 (1998), 205–219.
8. Minden, A., Karin, M., Regulation and function of the JNK subgroup of MAP kinases. Biochim. Biophys. Acta 1333 (1997), F85–F104.
9. Cui, J., Zhang, M., Zhang, Y., Xu, Z., JNK pathway: diseases and therapeutic potential. Acta Pharmacol. Sin. 28 (2007), 601–608.
10. Gupta, S., Barrett, T., Whitmarsh, A.J., Cavanagh, J., Sluss, H.K., Derijard, B., Davis, R.J., Selective interaction of JNK protein kinase isoforms with transcription factors. EMBO J. 15 (1996), 2760–2770.
11. Lawler, S., Fleming, Y., Goedert, M., Cohen, P., Synergistic activation of SAPK1/JNK1 by two MAP kinase kinases in vitro. Curr. Biol. 8 (1998), 1387–1390.
12. Johnson, G.J., The c-Jun kinase/stress-activated pathway: regulation, function and role in human disease. Biochim. Biophys. Acta 1773 (2007), 1341–1348.
13. Lisnock, J., Griffin, P., Calaycay, J., Frantz, B., Parsons, J., O'Keefe, S.J., LoGrasso, P., Activation of JNK3 alpha 1 requires both MKK4 and MKK7: kinetic characterization of in vitro phosphorylated JNK3 alpha 1. Biochemistry 39 (2000), 3141–3148.
14. Kishimoto, H., Nakagawa, K., Watanabe, T., Kitagawa, D., Momose, H., Seo, J., Nishitai, G., Shimizu, N., Ohata, S., Tanemura, S., Asaka, S., Goto, T., Fukushi, H., Yoshida, H., Suzuki, A., Sasaki, T., Wada, T., Penninger, J.M., Nishina, H., Katada, T., Different properties of SEK1 and MKK7 in dual phosphorylation of stress-induced activated protein kinase SAPK/JNK in embryonic stem cells. J. Biol. Chem. 278 (2003), 16595–16601.
15. Fleming, Y., Armstrong, C.G., Morrice, N., Paterson, A., Goedert, M., Cohen, P., Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7. Biochem. J. 352 (2000), 145–154.
16. Zhao, Q., Chen, P., Manson, M.E., Liu, Y., Production of active recombinant mitogen-activated protein kinases through transient transfection of 293T cells. Protein Expr. Purif. 46 (2006), 468–474.
17. Khokhlatchev, A., Xu, S., English, J., Wu, P., Schaefer, E., Cobb, M.H., Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria. Efficient synthesis of active protein kinases. J. Biol. Chem. 272 (1997), 11057–11062.
18. Thévenin, A.F., Zony, C.L., Bahnson, B.J., Colman, R.F., Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts. Protein Expr. Purif. 75 (2011), 138–146.
19. Savopoulos, J.W., Dowd, S., Armour, C., Carter, P.S., Greenwood, C.J., Mills, D., Powell, D., Pettman, G.R., Jenkins, O., Walsh, F.S., Philpott, K.L., Expression and purification of functional JNK2beta2: perspectives on high-level production of recombinant MAP kinases. Protein Expr. Purif. 24 (2002), 25–32.
20. Fuhrmann, M., Hausherr, A., Ferbitz, L., Schödl, T., Heitzer, M., Hegemann, P., Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene. Plant Mol. Biol. 55 (2004), 869–881.
21. Angov, E., Hillier, C.J., Kincaid, R.L., Lyon, J.A., Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression host. PLoS Biol. 3 (2008), 1–10.
22. Leach, F.R., Webster, J.J., Commercially available firefly luciferase reagents. Methods Enzymol. 133 (1986), 51–70.
23. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970), 680–685.
24. Schägger, H., von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166 (1987), 368–379.
25. S⊘rensen, H.P., Mortensen, K.K., Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microb. Cell Fact., 4, 2005, 1.
26. S⊘rensen, H.P., Mortensen, K.K., Advanced genetic strategies for recombinant protein expression in Escherichia coli. J. Biotechnol. 115 (2005), 113–128.
27. Kurland, C., Gallant, J., Errors of heterologous protein expression. Curr. Opin. Biotechnol. 7 (1996), 489–493.
28. Thanaraj, T.A., Argos, P., Ribosome-mediated translational pause and protein domain organization. Protein Sci. 5 (1996), 1594–1612.
29. Fath, S., Bauer, A.P., Liss, M., Spriestersbach, A., Maertens, B., Hahn, P., Ludwig, C., Schäfer, F., Graf, M., Wagner, R., Multiparameter RNA and codon optimization: A standardized tool to assess and enhance autologous mammalian gene expression. PLoS ONE, 6, 2011, e17596.
30. Georgiou, G., Valax, P., Expression of correctly folded proteins in Escherichia coli. Curr. Opin. Biotechnol. 7 (1996), 190–197.
31. Piatak, M., Lane, J.A., Laird, W., Bjorn, M.J., Wang, A., Williams, M., Expression of soluble and fully functional ricin A chain in Escherichia coli is temperature-sensitive. J. Biol. Chem. 263 (1988), 4837–4843.
32. Guan, Z., Buckman, S.Y., Pentland, A.P., Templeton, D.J., Morrison, A.R., Induction of cyclooxygenase-2 by the activated MEKK1 → SEK1/MKK4 → p38 mitogen-activated protein kinase pathway. J. Biol. Chem. 273 (1998), 12901–12908.
33. Shrestha, A., Hamilton, G., O'Neill, E., Knapp, S., Elkins, J.M., Analysis of conditions affecting auto-phosphorylation of human kinases during expression in bacteria. Protein Expr. Purif. 81 (2012), 136–143.
34. Seeliger, M.A., Young, M., Henderson, M.N., Pellicena, P., King, D.S., Falick, A.M., Kuriyan, J., High yield bacterial expression of active c-Abl and c-Src tyrosine kinases. Protein Sci. 14 (2005), 3135–3139.
35. Kinstrie, R., Luebbering, N., Diego, M.-S., Sibbet, G., Han, J., Lochhead, P.A., Cleghon, V., Characterization of a domain that transiently converts class 2 DYRKs into intramolecular tyrosine kinases. Sci. Signal., 3, 2009, ra16.
36. Macek, B., Gnad, F., Soufi, B., Kumar, C., Olsen, J.V., Mijakovic, I., Mann, M., Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell. Proteomics 2 (2008), 299–307.
37. 2-terminal kinase are expressed in primary glial tumors. Cancer Res. 63 (2003), 250–255.
38. Cui, J., Holgado-Madruga, M., Su, W., Tsuiki, H., Wedegaertner, P., Wong, A.J., Identification of a specific domain responsible for JNK2α2 autophosphorylation. J. Biol. Chem. 280 (2005), 9913–9920.
39. Figuera-Losada, M., LoGrasso, P.V., Enzyme kinetics and interaction studies for human JNK1β1 and substrates activating transcription factor 2 (ATF2) and c-Jun N-terminal kinase (c-Jun). J. Biol. Chem. 287 (2012), 13291–13302.