Analysis of conditions affecting auto-phosphorylation of human kinases during expression in bacteria

Protein Expression and Purification

Volumn 81, Issue 1, 2012, Pages 136-143

  Shrestha, Amit ^a   Hamilton, Garth ^a   O'Neill, Eric ^a   Knapp, Stefan ^a   Elkins, Jonathan M ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Auto phosphorylation; Expression; Kinase; Purification

Indexed keywords

PROTEIN SERINE THREONINE KINASE; RECOMBINANT PROTEIN;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR CLONING; PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN TERTIARY STRUCTURE;

CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; MASS SPECTROMETRY; MODELS, MOLECULAR; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 80755176772     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.09.012     Document Type: Article

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