Development of a high-throughput gaussia luciferase reporter assay for the activation of the GADD45a gene by mutagens, promutagens, clastogens, and aneugens

Journal of Biomolecular Screening

Volumn 17, Issue 10, 2012, Pages 1302-1315

  Hughes, Chris ^a   Rabinowitz, Adam ^a   Tate, Matthew ^a   Birrell, Louise ^a   Allsup, Jodie ^a   Billinton, Nicholas ^a   Walmsley, Richard M ^b  

^a Gentronix Ltd   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

aneugen; BlueScreen; clastogen; GADD45a; genotoxicity; genotoxin; GreenScreen; HTS; luciferase; mutagen; promutagen; screening

Indexed keywords

ANEUGEN; CLASTOGEN; CYANINE DYE; GREEN FLUORESCENT PROTEIN; GROWTH ARREST AND DNA DAMAGE INDUCIBLE PROTEIN 45; LIVER EXTRACT; LUCIFERASE; MUTAGENIC AGENT; PROMUTAGEN; THIAZOLE ORANGE;

ARTICLE; CELL DENSITY; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; GENE; GENE ACTIVATION; GENOTOXICITY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LYMPHOBLASTOID CELL LINE; OPTICS; PRIORITY JOURNAL; SENSITIVITY ANALYSIS;

BENZOTHIAZOLES; CELL CYCLE PROTEINS; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; GENES, REPORTER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; HYDROGEN-ION CONCENTRATION; LUCIFERASES; LUMINESCENCE; MUTAGENICITY TESTS; MUTAGENS; NUCLEAR PROTEINS; QUINOLINES; REPRODUCIBILITY OF RESULTS; SMALL MOLECULE LIBRARIES; TRANSCRIPTIONAL ACTIVATION;

MAMMALIA;

EID: 84869069268     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057112453312     Document Type: Article

References (29)

1. ICH S2(R1): Guidance on Genotoxicity Testing and Data Interpretation for Pharmaceuticals Intended for Human Use. www.ich.org/fileadmin/Public-Web-Site/ ICH-Products/Guidelines/Safety/S2-R1/Step4/S2R1-Step4.pdf
2. Burke D. A., Wedd D. J., Burlinson B.. Use of the Miniscreen Assay to Screen Novel Compounds for Bacterial Mutagenicity in the Pharmaceutical Industry. Mutagenesis. 1996 ; 11: 201-205
3. Kirkland D., Aardema M., Henderson L., Muller L.. Evaluation of the Ability of a Battery of Three In Vitro Genotoxicity Tests to Discriminate Rodent Carcinogens and Non-carcinogens, I: Sensitivity, Specificity and Relative Predictivity. Mutat. Res. 2005 ; 584: 1-256
4. Matthews E. J., Kruhlak N. L., Cimino M. C., Benz R. D., Contrera J. F.. An Analysis of Genetic Toxicity, Reproductive and Developmental Toxicity, and Carcinogenicity Data, I: Identification of Carcinogens Using Surrogate Endpoints. Regul. Toxicol. Pharmacol. 2006 ; 44: 83-96
5. Kirkland D., Pfuhler S., Tweats D., Aardema M., Corvi R., Darroudi F., Elhajouji A., Glatt H.-R., Hastwell P., Hayashi M., et al. How to Reduce False Positive Results When Undertaking In Vitro Genotoxicity Testing and Thus Avoid Unnecessary Follow-Up Animal Tests: Report of an ECVAM Workshop. ; 2007 ; 2007. 31.
6. Kirkland D., Kasper P., Müller L., Corvi R., Speit G.. Recommended Lists of Genotoxic and Non-genotoxic Chemicals for Assessment of the Performance of New or Improved Genotoxicity Tests: A Follow-up to an ECVAM Workshop. ; 2008 ; 2008. 99.
7. Thybaud V., Aardema M., Casciano D., Dellarco V., Embry M. R., Gollapudi B. B., Hayashi M., Holsapple M. P., Jacobson-Kram D., Kasper P., et al. Relevance and Follow-up of Positive Results in In Vitro Genetic Toxicity Assays: An ILSI-HESI Initiative. Mutat. Res. 2007 ; 633: 67-79
8. Guidance on a Strategy for Genotoxicity Testing of Chemical Substances. http://iacom.org.uk/guidstate/documents/COMGuidanceFINAL.pdf. Accessed January 2012.
9. Hastwell P. W., Chai L. L., Roberts K. J., Webster T. W., Harvey J. S., Rees R. W., Walmsley R. M.. High-Specificity and High-Sensitivity Genotoxicity Assessment in a Human Cell Line: Validation of the GreenScreen HC GADD45a-GFP Genotoxicity Assay. Mutat. Res. 2006 ; 607: 160-175
10. Hastwell P. W., Webster T. W., Tate M., Billinton N., Lynch A. M., Harvey J. S., Rees R. W., Walmsley R. M.. Analysis of 75 Marketed Pharmaceuticals Using the GADD45a-GFP 'GreenScreen HC' Genotoxicity Assay. Mutagenesis. 2009 ; 24: 455-463
11. Jagger C., Tate M., Cahill P. A., Hughes C., Knight A. W., Billinton N., Walmsley R. M.. Assessment of the Genotoxicity of S9-generated Metabolites Using the GreenScreen HC GADD45a-GFP Assay. Mutagenesis. 2009 ; 24: 35-509
12. Birrell L., Cahill P., Hughes C., Tate M., Walmsley R. M.. GADD45a-GFP GreenScreen HC Assay Results for the ECVAM Recommended Lists of Genotoxic and Non-genotoxic Chemicals for Assessment of New Genotoxicity Tests. Mutat. Res. 2010 ; 695: 87-95
13. Billinton N., Hastwell P. W., Beerens D., Birrell L., Ellis P., Maskell S., Webster T. W., Windebank S., Woestenborghs F., Lynch A. M., et al. Inter-laboratory Assessment of the GreenScreen HC GADD45a-GFP Genotoxicity Screening Assay: An Enabling Study for Independent Validation as an Alternative Method. Mutat. Res. 2008 ; 654: 23-33
14. Billinton N., Bruce S., Hansen J. R., Hastwell P. W., Jagger C., McComb C., Klug M. L., Pant K., Rabinowitz A., Rees R., et al. A Pre-validation Transferability Study of the GreenScreen HC GADD45a-GFP Assay with a Metabolic Activation System (S9). Mutat. Res. 2010 ; 700: 44-50
15. Fan F., Wood K. V.. Bioluminescent Assays for High-Throughput Screening. Assay Drug Dev. Technol. 2007 ; 5: 127-136
16. Verschaeve L., Van Gompel J., Thilemans L., Regniers L., Vanparys P., van der Lelie D.. VITOTOX Bacterial Genotoxicity and Toxicity Test for the Rapid Screening of Chemicals. Environ. Mol. Mutagen. 1999 ; 33: 240-248
17. Ohno K., Ishihata K., Tanaka-Azuma Y., Yamada T.. A Genotoxicity Test System Based on p53R2 Gene Expression in Human Cells: Assessment of Its Reactivity to Various Classes of Genotoxic Chemicals. Mutat. Res. 2008 ; 656: 27-35
18. Ignowski J. M., Schaffer D. V.. Kinetic Analysis and Modeling of Firefly Luciferase as a Quantitative Reporter Gene in Live Mammalian Cells. Biotechnol. Bioeng. 2004 ; 86: 827-834
19. Tannous B. A., Kim D. E., Fernandez J. L., Weissleder R., Breakefield X. O.. Codon-optimized Gaussia Luciferase cDNA for Mammalian Gene Expression in Culture and In Vivo. Mol. Ther. 2005 ; 11: 435-443
20. Billinton N., Knight A. W.. Seeing the Wood through the Trees: A Review of Techniques for Distinguishing Green Fluorescent Protein from Endogenous Autofluorescence. Anal. Biochem. 2001 ; 291: 175-197
21. Knight A. W., Goddard N. J., Billinton N., Cahill P. A., Walmsley R. M.. Fluorescence Polarization Discriminates Green Fluorescent Protein from Interfering Autofluorescence in a Microplate Assay for Genotoxicity. J. Biochem. Bioph. Meth. 2002 ; 51: 165-177
22. Teranishi K., Shimomura O.. Solubilizing Coelenterazine in Water with Hydroxypropyl-ß-cyclodextrin. Biosci. Biotech. Biochem. 1997 ; 61: 1219-1220
23. Dubuisson M. L. N., Rees J.-F., Marchand-Brynaert J., Coelenterazine J.. (Marine Bioluminescent Substrate): A Source of Inspiration for the Discovery of Novel Antioxidants. Drug Dev. Ind. Pharm. 2005 ; 31: 827-849
24. OhmiyaY.WuC.Stabilizing Composition and Stabilizing Method of Coelenterazine Solution for High-Throughput Measurement of Luciferase Activity. U.S. Patent 7718389, May18, 2010.
25. Lee L. G., Chia-Huei Chen C.-H., Chiu L. A.. Thiazole Orange: A New Dye for Reticulocyte Analysis. Cytometry. 1986 ; 7: 508-517
26. Zhang J.-H., Chung T. D.Y., Oldenburg K. R.. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J. Biomol. Screen. 1999 ; 4: 67-73
27. Martelli A., Matioli S., Pastorino G., Robbiano L., Allavena A., Brambilla G.. Genotoxicity Testing of Chloramphenicol in Rodent and Human Cells. Mutat. Res. 1991 ; 260: 65-72
28. Lu J., Jin T., Nordberg G., Nordberg M.. Metallothionein Gene Expression in Peripheral Lymphocytes from Cadmium-Exposed Workers. Cell Stress Chaperon. 2001 ; 6: 97-104
29. Halliwell B.. Are Polyphenols Antioxidants or Pro-oxidants?. Arch. Biochem. Biophys. 2008 ; 476: 107-112