Localization of poly(3-Hydroxybutyrate) (PHB) granule-associated proteins during PHB granule formation and identification of two new phasins, phap6 and phap7, in Ralstonia eutropha H16

Journal of Bacteriology

Volumn 194, Issue 21, 2012, Pages 5909-5921

  Pfeiffer, Daniel ^a   Jendrossek, Dieter ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; H 16 B1988 PROTEIN; H 16 B2296 PROTEIN; H 16 B2326 PROTEIN; POLY(3 HYDROXYBUTYRIC ACID); UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CHROMOSOME DELETION; CUPRIAVIDUS NECATOR; DNA SEQUENCE; GENE SEQUENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN MOTIF; SEPTUM NUCLEUS; WILD TYPE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CUPRIAVIDUS NECATOR; DNA-BINDING PROTEINS; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HYDROXYBUTYRATES; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; POLYESTERS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT;

CUPRIAVIDUS NECATOR;

EID: 84868620054     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00779-12     Document Type: Article

References (55)

1. Abe T, Kobayashi T, Saito T. 2005. Properties of a novel intracellular poly(3-hydroxybutyrate) depolymerase with high specific activity (PhaZd) in Wautersia eutropha H16. J. Bacteriol. 187:6982- 6990.
2. Beeby M, Cho M, Stubbe J, Jensen GJ. 2012. Growth and localization of polyhydroxybutyrate granules in Ralstonia eutropha. J. Bacteriol. 194: 1092-1099.
3. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248 -254.
4. Brandl H, Gross RA, Lenz RW, Fuller RC. 1988. Pseudomonas oleovorans as a source of poly(beta-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol. 54:1977-1982.
5. Brigham CJ, et al. 2010. Elucidation of beta-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression. J. Bacteriol. 192:5454 -5464.
6. Cai S, et al. 2012. Identification of the haloarchaeal phasin (PhaP) that functions in polyhydroxyalkanoate accumulation and granule formation in Haloferax mediterranei. Appl. Environ. Microbiol. 78:1946 -1952.
7. Chen G-Q. 2009. A microbial polyhydroxyalkanoates (PHA) based bioand materials industry. Chem. Soc. Rev. 38:2434 -2446.
8. Cho M, Brigham CJ, Sinskey AJ, Stubbe J. 2012. Purification of a polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications in the initiation and elongation of polymer formation in vivo. Biochemistry 51:2276 -2288.
9. Escapa IF, García JL, Bühler B, Blank LM, Prieto MA. 2012. The polyhydroxyalkanoate metabolism controls carbon and energy spillage in Pseudomonas putida. Environ. Microbiol. 14:1049 -1063.
10. Galán B, et al. 2011. Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442. Mol. Microbiol. 79:402- 418.
11. Gerngross TU, Reilly P, Stubbe J, Sinskey AJ, Peoples OP. 1993. Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules. J. Bacteriol. 175:5289 -5293.
12. Grage K, et al. 2009. Bacterial polyhydroxyalkanoate granules: biogenesis, structure, and potential use as nano-/micro-beads in biotechnological and biomedical applications. Biomacromolecules 10:660-669.
13. Han J, et al. 2010. Wide distribution among halophilic archaea of a novel polyhydroxyalkanoate synthase subtype with homology to bacterial type III synthases. Appl. Environ. Microbiol. 76:7811-7819.
14. Handrick R, Reinhardt S, Jendrossek D. 2000. Mobilization of poly(3-hydroxybutyrate) in Ralstonia eutropha. J. Bacteriol. 182:5916 -5918.
15. Hermawan S, Jendrossek D. 2007. Microscopical investigation of poly(3-hydroxybutyrate) granule formation in Azotobacter vinelandii. FEMS Microbiol. Lett. 266:60-64.
16. Jendrossek D. 2005. Fluorescence microscopical investigation of poly(3-hydroxybutyrate) granule formation in bacteria. Biomacromolecules 6:598-603.
17. Jendrossek D. 2009. Polyhydroxyalkanoate granules are complex subcellular organelles (carbonosomes). J. Bacteriol. 191:3195-3202.
18. Jendrossek D, Handrick R. 2002. Microbial degradation of polyhydroxyalkanoates. Annu. Rev. Microbiol. 56:403- 432.
19. Jendrossek D, Selchow O, Hoppert M. 2007. Poly(3-hydroxybutyrate) granules at the early stages of formation are localized close to the cytoplasmic membrane in Caryophanon latum. Appl. Environ. Microbiol. 73: 586-593.
20. Kirstein J, Strahl H, Molière N, Hamoen LW, Turgay K. 2008. Localization of general and regulatory proteolysis in Bacillus subtilis cells. Mol. Microbiol. 70:682- 694.
21. Kobayashi T, Shiraki M, Abe T, Sugiyama A, Saito T. 2003. Purification and properties of an intracellular 3-hydroxybutyrate-oligomer hydrolase (PhaZ2) in Ralstonia eutropha H16 and its identification as a novel intracellular poly(3-hydroxybutyrate) depolymerase. J. Bacteriol. 185:3485- 3490.
22. Kobayashi T, Uchino K, Abe T, Yamazaki Y, Saito T. 2005. Novel intracellular 3-hydroxybutyrate-oligomer hydrolase in Wautersia eutropha H16. J. Bacteriol. 187:5129 -5135.
23. Kuchta K, Chi L, Fuchs H, Pötter M, Steinbüchel A. 2007. Studies on the influence of phasins on accumulation and degradation of PHB and nanostructure of PHB granules in Ralstonia eutropha H16. Biomacromolecules 8:657- 662.
24. Lenz O, Friedrich B. 1998. A novel multicomponent regulatory system mediatesH2sensing in Alcaligenes eutrophus. Proc. Natl. Acad. Sci. U. S. A. 95:12474 -12479.
25. Madison LL, Huisman GW. 1999. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 63: 21-53.
26. Nakamura S, Fukui T, Mifune J. 2008. Targeted engineering of Cupriavidus necator chromosome for bio-synthesis of poly(3-hydroxybutyrateco-3-hydroxyhexanoate) from vegetable oil. Can. J. Chem. 86:621- 627.
27. Neumann L, et al. 2008. Binding of the major phasin, PhaP1, from Ralstonia eutropha H16 to poly(3-hydroxybutyrate) granules. J. Bacteriol. 190:2911-2919.
28. Pederson EN, McChalicher CWJ, Srienc F. 2006. Bacterial synthesis of PHA block copolymers. Biomacromolecules 7:1904 -1911.
29. Peoples OP, Sinskey AJ. 1989. Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264:15298 -15303.
30. Peplinski K, et al. 2010. Genome-wide transcriptome analyses of the "Knallgas" bacterium Ralstonia eutropha H16 with regard to polyhydroxyalkanoate metabolism. Microbiology 156:2136 -2152.
31. Pfeiffer D, Jendrossek D. 2011. Interaction between poly(3-hydroxybutyrate) granule-associated proteins as revealed by two-hybrid analysis and identification of a new phasin in Ralstonia eutropha H16. Microbiology 157:2795-2807.
32. Pfeiffer D, Wahl A, Jendrossek D. 2011. Identification of a multifunctional protein, PhaM, that determines number, surface to volume ratio, subcellular localization and distribution to daughter cells of poly(3-hydroxybutyrate), PHB, granules in Ralstonia eutropha H16. Mol. Microbiol. 82:936 -951.
33. Pohlmann A, et al. 2006. Genome sequence of the bioplastic-producing "Knallgas" bacterium Ralstonia eutropha H16. Nat. Biotechnol. 24:1257- 1262.
34. Pötter M, Steinbüchel A. 2006. Biogenesis and Structure of polyhydroxyalkanoate granules. Microbiol. Monogr. 1:110 -136.
35. Pötter M, Madkour MH, Mayer F, Steinbüchel A. 2002. Regulation of phasin expression and polyhydroxyalkanoate (PHA) granule formation in Ralstonia eutropha H16. Microbiology 148:2413-2426.
36. Pötter M, et al. 2004. The complex structure of polyhydroxybutyrate (PHB) granules: four orthologous and paralogous phasins occur in Ralstonia eutropha. Microbiology 150:2301-2311.
37. Rehm BHA. 2003. Polyester synthases: natural catalysts for plastics. Biochem. J. 376:15-33.
38. Rehm BHA, Antonio RV, Spiekermann P, Amara AA, Steinbüchel A. 2002. Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: in vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model. Biochim. Biophys. Acta 1594:178 -190.
39. Saegusa H, Shiraki M, Kanai C, Saito T. 2001. Cloning of an intracellular poly[D(-)-3-hydroxybutyrate] depolymerase gene from Ralstonia eutropha H16 and characterization of the gene product. J. Bacteriol. 183:94-100.
40. Schlegel HG, Lafferty R, Krauss I. 1970. The isolation of mutants not accumulating poly-beta-hydroxybutyric acid. Arch. Mikrobiol. 71:283-294.
41. Schubert P, Steinbüchel A, Schlegel HG. 1988. Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli. J. Bacteriol. 170:5837-5847.
42. Simon R, Priefer U, Pühler A. 1983. A broad host-range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gramnegative bacteria. Nat. Biotechnol. 1:784 -791.
43. Slater SC, Voige WH, Dennis DE. 1988. Cloning and expression in Escherichia coli of the Alcaligenes eutrophusH16poly-beta-hydroxybutyrate biosynthetic pathway. J. Bacteriol. 170:4431-4436.
44. Stubbe J, Tian J. 2003. Polyhydroxyalkanoate (PHA) hemeostasis: the role of PHA synthase. Nat. Prod. Rep. 20:445- 457.
45. Stubbe J, et al. 2005. Nontemplate-dependent polymerization processes: polyhydroxyalkanoate synthases as a paradigm. Annu. Rev. Biochem. 74: 433-480.
46. Sudesh K, Abe H, Doi Y. 2000. Synthesis, structure and properties of polyhydroxyalkanoates: biological polymers. Prog. Polym. Sci. 25:1503- 1555.
47. Tian J, et al. 2005. Analysis of transient polyhydroxybutyrate production in Wautersia eutropha H16 by quantitative Western analysis and transmission electron microscopy. J. Bacteriol. 187:3825-3832.
48. Tian J, Sinskey AJ, Stubbe J. 2005. Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase. Biochemistry 44:1495-1503.
49. Tian J, Sinskey AJ, Stubbe J. 2005. Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy. J. Bacteriol. 187:3814 -3824.
50. Tsumoto K, Umetsu M, Kumagai I, Ejima D, Arakawa T. 2003. Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine. Biochem. Biophys. Res. Commun. 312:1383-1386.
51. Uchino K, Saito T, Jendrossek D. 2008. Poly(3-hydroxybutyrate) (PHB) depolymerase PhaZa1 is involved in mobilization of accumulated PHB in Ralstonia eutropha H16. Appl. Environ. Microbiol. 74: 1058 -1063.
52. Wieczorek R, Pries A, Steinbüchel A, Mayer F. 1995. Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. J. Bacteriol. 177:2425-2435.
53. York GM, et al. 2003. Ralstonia eutropha H16 encodes two and possibly three intracellular poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J. Bacteriol. 185:3788 -3794.
54. York GM, Stubbe J, Sinskey AJ. 2002. The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J. Bacteriol. 184:59-66.
55. Yuan W, et al. 2001. Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies. Arch. Biochem. Biophys. 394:87-98.