Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: In vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1594, Issue 1, 2002, Pages 178-190

  Rehm, Bernd H A ^a   Antonio, Regina V ^a   Spiekermann, Patricia ^a   Amara, Amro A ^a   Steinbüchel, Alexander ^a  

^a UNIVERSITY HOSPITAL MÜNSTER   (Germany)

Author keywords

In vitro evolution; Mutagenesis; Polyhydroxyalkanoate; Polyhydroxyalkanoate synthase; Polyhydroxybutyrate; Ralstonia eutropha; Threading model

Indexed keywords

AMINO ACID; HYBRID PROTEIN; MUTANT PROTEIN; POLY(3 HYDROXYBUTYRIC ACID)SYNTHASE; POLYHYDROXYALKANOATE SYNTHASE; SYNTHETASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; EVOLUTION; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SITE DIRECTED MUTAGENESIS; WAUTERSIA EUTROPHA;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; BINDING SITES; CUPRIAVIDUS NECATOR; EVOLUTION, MOLECULAR; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

BURKHOLDERIA; BURKHOLDERIA GLUMAE; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; RALSTONIA; WAUTERSIA EUTROPHA;

EID: 0037203912     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(01)00299-0     Document Type: Article

References (50)