Poly(3-hydroxybutyrate) (PHB) depolymerase PhaZa1 is involved in mobilization of accumulated PHB in Ralstonia eutropha H16

Applied and Environmental Microbiology

Volumn 74, Issue 4, 2008, Pages 1057-1063

  Uchino, Keiichi ^a,b   Saito, Terumi ^b   Jendrossek, Dieter ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b KANAGAWA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PHB DEPOLYMERASES; PHYSIOLOGICAL FUNCTION;

DEPOLYMERIZATION; ESCHERICHIA COLI; GENE EXPRESSION; MUTAGENESIS; PHYSIOLOGICAL MODELS;

ENZYME IMMOBILIZATION;

AMINO ACID DERIVATIVE; GENE PRODUCT; POLY(3 HYDROXYBUTYRIC ACID); POLY(3 HYDROXYBUTYRIC ACID) PHAZA1; UNCLASSIFIED DRUG; 3 HYDROXYBUTYRIC ACID; CARBOXYLESTERASE; GLUCOSE;

AMINO ACID; BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; GENOME; MOBILIZATION;

ARTICLE; BACTERIAL GENETICS; CUPRIAVIDUS NECATOR; DEPOLYMERIZATION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE; GENE SEQUENCE; NONHUMAN; PHAZA1 GENE; PHAZB GENE; PHAZC GENE; PHAZD1 GENE; PHYSIOLOGY; COMPARATIVE STUDY; ENZYMOLOGY; FLUORESCENCE MICROSCOPY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; PH; WESTERN BLOTTING;

CUPRIAVIDUS NECATOR; ESCHERICHIA COLI;

3-HYDROXYBUTYRIC ACID; BLOTTING, WESTERN; CARBOXYLIC ESTER HYDROLASES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; GLUCOSE; HYDROGEN-ION CONCENTRATION; MICROSCOPY, FLUORESCENCE;

EID: 39649110362     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02342-07     Document Type: Article

References (43)

1. Abe, T., T. Kobayashi, and T. Saito. 2005. Properties of a novel intracellular poly(3-hydroxybutyrate) depolymerase with high specific activity (PhaZd) in Wautersia eutropha H16. J. Bacteriol. 187:6982-6990.
2. Brandl, H., R. A. Gross, R. W. Lenz, and R. C. Fuller. 1988. Pseudomonas oleovorans as a source of poly(β-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol. 54:1977-1982.
3. de Eugenio, L. I., P. Garcia, J. M. Luengo, J. M. Sanz, J. S. Roman, J. L. Garcia, and M. A. Prieto. 2007. Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: characterization of a paradigmatic enzyme. J. Biol. Chem. 282:4951-4962.
4. Foster, L. J. R., R. W. Lenz, and R. C. Fuller. 1999. Intracellular depolymerase activity in isolated inclusion bodies containing polyhydroxyalkanoateswith long alkyl and functional substituents in the side chain. Int. J. Biol. Macromol. 26:187-192.
5. Gao, D., A. Maehara, T. Yamane, and S. Ueda. 2001. Identification of the intracellular polyhydroxyalkanoate depolymerase gene of Paracoccus deniirificans and some properties of the gene product. FEMS Microbiol. Lett. 196:159-164.
6. Gebauer, B., and D. Jendrossek. 2006. Assay of poly(3-hydroxybutyrate) depolymerase activity and product determination. Appl. Environ. Microbiol. 72:6094-6100.
7. Handrick, R., S. Reinhardt, M. L. Focarete, M. Scandola, G. Adamus, M. Kowalczuk, and D. Jendrossek. 2001. A new type of thermoalkalophilic hydrolase of Paucimonas lemoignei with high specificity for amorphous polyesters of short chain-length hydroxyalkanoic acids. J. Biol. Chem. 276:36215-36224.
8. Handrick, R., S. Reinhardt, and D. Jendrossek. 2000. Mobilization of poly(3-hydroxybutyrate) in Ralstonia eutropha. J. Bacteriol. 182:5916-5918.
9. Handrick, R., S. Reinhardt, P. Kimmig, and D. Jendrossek. 2004. The "intracellular" poly(3-hydroxybutyrate) (PHB) depolymerase of Rhodospirillum rubrum is a periplasm-located protein with specificity for native PHB and with structural similarity to extracellular PHB depolymerases. J. Bacteriol. 186:7243-7253.
10. Handrick, R., S. Reinhardt, D. Schultheiss, T. Reichart, D. Schuler, V. Jendrossek, and D. Jendrossek. 2004. Unraveling the function of the Rhodospirillum rubrum activator of polyhydroxybutyrate (PHB) degradation: the activator is a PHB-granule-bound protein (phasin). J. Bacteriol. 186:2466-2475.
11. Handrick, R., U. Technow, T. Reichart, S. Reinhardt, T. Sander, and D. Jendrossek. 2004. The activator of the Rhodospirillum rubrum PHB depolymerase is a polypeptide that is extremely resistant to high temperature (121 degrees C) and other physical or chemical stresses. FEMS Microbiol. Lett. 230:265-274.
12. Hermawan, S., and D. Jendrossek. 2007. Microscopical investigation of poly(3-hydroxybutyrate) granule formation in Azotobacter vinelandii. FEMS Microbiol. Lett. 266:60-64.
13. Huisman, G. W., E. Wonink, R. Meima, B. Kazemier, P. Terpstra, and B. Witholt. 1991. Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA. J. Biol. Chem. 266:2191-2198.
14. Jendrossek, D. 2002. Extracellular polyhydroxyalkanoate depolymerases: the key enzymes of PHA degradation, p. 41-83. In Y. Doi and A. Steinbüchel (ed.), Biopolymers, vol. 3b. Polyesters II. Wiley-VCH, Hoboken, NJ.
15. Jendrossek, D. 2007. Peculiarities of PHA granules preparation and PHA depolymerase activity determination. Appl. Microbiol. Biotechnol. 74:1186-1196.
16. Jendrossek, D., A. Frisse, A. Behrends, M. Andermann, H. D. Kratzin, T. Stanislawski, and H. G. Schlegel. 1995. Biochemical and molecular characterization of the Pseudomonas lemoignei polyhydroxyalkanoate depolymerase system. J. Bacteriol. 177:596-607.
17. Jendrossek, D., and R. Handrick. 2002. Microbial degradation of polyhydroxyalkanoates. Annu. Rev. Microbiol. 56:403-432.
18. Jendrossek, D., A. Schirmer, and H. G. Schlegel. 1996. Biodegradation of polyhydroxyalkanoic acids. Appl. Microbiol. Biotechnol. 46:451-463.
19. Kobayashi, T., M. Shiraki, T. Abe, A. Sugiyama, and T. Saito. 2003. Purification and properties of an intracellular 3-hydroxybutyrate-oligomer hydrolase (PhaZ2) in Ralstonia eutropha H16 and its identification as a novel intracellular poly(3-hydroxybutyrate) depolymerase. J. Bacteriol. 185:3485-3490.
20. Kobayashi, T., K. Uchino, T. Abe, Y. Yamazaki, and T. Saito. 2005. Novel intracellular 3-hydroxybutyrate-oligomer hydrolase in Wautersia eutropha H16. J. Bacteriol. 187:5129-5135.
21. Lee, S.-Y., and Y. Lee. June 2003. (R)-Hydroxycarboxylic acid producing recombinant microorganisms and process for preparing (R)-hydroxycarboxylic acid using the same. WIPO patent WO03/046159.
22. Lee, S. Y., and N. H. Chang. 1993. High cell density cultivation of Escherichia coli using sucrose as a carbon source. Biotechnol. Lett. 15:971-974.
23. Lee, S. Y., and Y. Lee. 2003. Metabolic engineering of Escherichia coli for production of enantiomerically pure (R)-(-)-hydroxycarboxylic acids. Appl. Environ. Microbiol 69:3421-3426.
24. Lee, S. Y., Y. Lee, and F. Wang. 1999. Chiral compounds from bacterial polyesters: sugars to plastics to fine chemicals. Biotechnol. Bioeng. 65:363-368.
25. Merrick, J. M., and M. Doudoroff. 1964. Depolymcrization of poly-β-hydroxybutyrate by intracellular enzyme system. J. Bacteriol. 88:60-71.
26. Merrick, J. M., D. G. Lundgren, and R. M. Pfister. 1965. Morphological changes in poly-beta-hydroxybutyrate granules associated with decreased susceptibility to enzymatic hydrolysis. J. Bacteriol. 89:234-239.
27. Merrick, J. M., R. Steger, and D. Dombroski. 1999. Hydrolysis of native poly(hydroxybutyrate) granules (PHB), crystalline PHB, and artificial amorphous PHB granules by intracellular and extracellular depolymerases. Int. J. Biol. Macromol. 25:129-134.
28. Park, S. J., S. Y. Lee, and Y. Lee. 2004. Biosynthesis of R-3-hydroxyalkanoic acids by metabolically engineered Escherichia coli. Appl. Biochem. Biotechnol. 113-116:373-379.
29. Pohlmann, A., W. F. Fricke, F. Reinecke, B. Kusian, H. Liesegang, R. Cramm, T. Eitinger, C. Ewering, M. Potter, E. Schwartz, A. Strittmatter, I. Voss, G. Gottschalk, A. Steinbuchel, B. Friedrich, and B. Bowien. 2006. Genome sequence of the bioplastic-producing "Knallgas" bacterium Ralstonia eutropha H16. Nat. Biotechnol. 24:1257-1262.
30. Ren, Q., A. Grubelnik, M. Hoerler, K. Ruth, R. Hartmann, H. Felber, and M. Zinn. 2005. Bacterial poly(hydroxyalkanoates) as a source of chiral hydroxyalkanoic acids. Biomacromolecules 6:2290-2298.
31. Ruth, K., A. Grubelnik, R. Hartmann, T. Egli, M. Zinn, and Q. Ren. 2007. Efficient production of (R)-3-hydroxycarboxylic acids by biotechnological conversion of polyhydroxyalkanoates and their purification. Biomacromolecules 8:279-286.
32. Saegusa, H., M. Shiraki, C. Kanai, and T. Saito. 2001. Cloning of an intracellular poly[D(-)-3-hydroxybutyrate] depolymerase gene from Ralstonia eutropha H16 and characterization of the gene product. J. Bacteriol. 183:94- 100.
33. Saegusa, H., M. Shiraki, and T. Saito. 2002. Cloning of an intracellular D(-)-3-hydroxybutyrate-oligomer hydrolase gene from Ralstonia eutropha H16 and identification of the active site serine residue by site-directed mutagenesis. J. Biosci. Bioeng. 94:106-112.
34. Saito, T., and T. Kobayashi. 2002. Intracellular degradation of PHAs, p. 23-40. In Y. Doi and A. Steinbüchel (ed.), Biopolymers, vol. 3b. Polyesters II. Wiley-VCH, Hoboken, NJ.
35. Schlegel, H. G., G. Gottschalk, and R. Von Bartha. 1961. Formation and utilization of poly-beta-hydroxybutyric acid by Knallgas bacteria (Hydrogenomonas). Nature 191:463-465.
36. Shinohe, T., M. Nojiri, T. Saito, T. Stanislawski, and D. Jendrossek. 1996. Determination of the active sites serine of the poly(3-hydroxybutyrate) depolymerases of Pseudomonas lemoignei (PhaZ5) and of Alcaligenes faecalis. FEMS Microbiol. Lett. 141:103-109.
37. Shiraki, M., T. Endo, and T. Saito. 2006. Fermentative production of (R)- (-)-3-hydroxybutyrate using 3-hydroxybutyrate dehydrogenase null mutant of Ralstonia eutropha and recombinant Escherichia coli. J. Biosci. Bioeng 102:529-534.
38. Shuster, C. W., and M. Doudoroff. 1962. A cold-sensitive D(-)beta-hydroxybutyric acid dehydrogenase from Rhodospirillum rubrum. J. Biol. Chem. 237:603-607.
39. Spiekermann, P., B. H. Rehm, R. Kalscheuer, D. Baumeister, and A Steinbüchel. 1999. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch. Microbiol. 171:73-80.
40. Tseng, C. L., H. J. Chen, and G. C. Shaw. 2006. Identification and characterization of the Bacillus thuringiensis phaZ gene, encoding new intracellular poly-3-hydroxybutyrate depolymerase. J. Bacteriol. 188:7592-7599.
41. Uchino, K., T. Saito, B. Gebauer, and D. Jendrossek. 2007. Isolated poly(3-hydroxybutyrate) (PHB) granules are complex bacterial organelles catalyzing formation of PHB from acetyl coenzyme A (CoA) and degradation of PHB to acetyl-CoA. J. Bacteriol. 189:8250-8256.
42. Wang, L., W. Armbruster, and D. Jendrossek. 2007. Production of medium-chain-length hydroxyalkanoic acids from Pseudomonas putida in pH stat. Appl. Microbiol. Biotechnol. 74:1047-1053.
43. York, G. M., J. Lupberger, J. Tian, A. G. Lawrence, J. Stubbe, and A. J. Sinskey. 2003. Ralstonia eutropha H16 encodes two and possibly three intracellular poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J. Bacteriol. 185:3788-3794.