Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine

Biochemical and Biophysical Research Communications

Volumn 312, Issue 4, 2003, Pages 1383-1386

  Tsumoto, Kouhei ^a   Umetsu, Mitsuo ^a   Kumagai, Izumi ^a   Ejima, Daisuke ^b   Arakawa, Tsutomu ^c  

^a TOHOKU UNIVERSITY   (Japan)

^b AJINOMOTO CO INC   (Japan)

^c ALLIANCE PROTEIN LABORATORIES   (United States)

Author keywords

Aggregation; Insoluble particle; L Arginine; Refolding

Indexed keywords

ARGININE; GREEN FLUORESCENT PROTEIN; GUANIDINE;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN EXPRESSION; SOLUBILITY; SOLUBILIZATION;

ESCHERICHIA COLI;

EID: 0344495223     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2003.11.055     Document Type: Article

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