Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase

Biochemistry

Volumn 44, Issue 5, 2005, Pages 1495-1503

  Tian, Jiamin ^a   Sinskey, Anthony J ^a   Stubbe, JoAnne ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MOLECULAR WEIGHT; POLYMERIZATION; RADIOGRAPHY; SYNTHESIS (CHEMICAL);

POLYHYDROXYBUTYRATE (PHB); SYNTHASE;

PROTEINS;

POLY(3 HYDROXYBUTYRIC ACID); PROTEIN PHAC; PROTEIN PHAE; PROTEIN POLYHYDROXYBUTYRATE SYNTHASE; PROTEIN SUBUNIT; SYNTHETASE; UNCLASSIFIED DRUG;

ACYLATION; AMINO ACID SEQUENCE; ARTICLE; AUTORADIOGRAPHY; COVALENT BOND; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVE SITE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MUTATIONAL ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN FUNCTION; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; TRANSMISSION ELECTRON MICROSCOPY; WAUTERSIA EUTROPHA; WESTERN BLOTTING;

ACYLTRANSFERASES; ALANINE; AMINO ACID SEQUENCE; ASPARTIC ACID; AUTORADIOGRAPHY; BACTERIAL PROTEINS; BLOTTING, WESTERN; BURKHOLDERIACEAE; CATALYSIS; CHROMATIACEAE; ENZYME STABILITY; HYDROXYBUTYRATES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; POLYMERS;

ALLOCHROMATIUM VINOSUM; WAUTERSIA EUTROPHA;

EID: 13444263508     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi047734z     Document Type: Article

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