Purification of polyhydroxybutyrate synthase from its native organism, ralstonia eutropha: Implications for the initiation and elongation of polymer formation in vivo

Biochemistry

Volumn 51, Issue 11, 2012, Pages 2276-2288

  Cho, Mimi ^a   Brigham, Christopher J ^a   Sinskey, Anthony J ^a   Stubbe, Joanne ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DRY WEIGHT; CLASS I; ELONGATION RATES; HIGH MOLECULAR WEIGHT; IN-VIVO; INITIATION RATE; LAG PHASE; MECHANISTIC STUDIES; POLYHYDROXYBUTYRATE; POLYMER FORMATION; RALSTONIA EUTROPHA; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SYNTHASES; WILD TYPES;

AFFINITY CHROMATOGRAPHY; BACTERIA; BIOLOGICAL MATERIALS; ELECTROPHORESIS; ELONGATION; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GRANULATION; POLYMERS; SODIUM; SODIUM SULFATE;

PURIFICATION;

POLYHYDROXYBUTYRATE SYNTHASE; POLYMER; SYNTHETASE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CUPRIAVIDUS NECATOR; ENZYME PURIFICATION; GEL PERMEATION CHROMATOGRAPHY; IN VIVO STUDY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ACYL COENZYME A; BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; GLYCOGEN SYNTHASE; HYDROXYBUTYRATES; KINETICS; MACROMOLECULAR SUBSTANCES;

CUPRIAVIDUS NECATOR; ESCHERICHIA COLI;

EID: 84858680303     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi2013596     Document Type: Article

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