Advanced microscale bioreactor system: A representative scale-down model for bench-top bioreactors

Cytotechnology

Volumn 64, Issue 6, 2012, Pages 667-678

  Hsu, Wei Ting ^a   Aulakh, Rigzen P S ^a   Traul, Donald L ^b   Yuk, Inn H ^a  

^a GENENTECH INC   (United States)

^b SARTORIUS STEDIM BIOTECH GMBH   (Germany)

Author keywords

Ambr ; Cell culture automation; Chinese hamster ovary; Scale down system; Single use bioreactor

Indexed keywords

DISSOLVED OXYGEN; GLUCOSE; LACTIC ACID;

AMBIENT AIR; ANIMAL CELL; ARTICLE; BIOREACTOR; CELL DENSITY; CELL METABOLISM; CELL VIABILITY; CHO CELL; CONTINUOUS STIRRED TANK REACTOR; ENVIRONMENTAL TEMPERATURE; FED BATCH CULTURE; GROWTH RATE; NONHUMAN; OSMOLALITY; OXYGEN CONSUMPTION; PH; PH MEASUREMENT; PROCESS DEVELOPMENT;

CRICETULUS GRISEUS;

EID: 84868461356     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-012-9446-1     Document Type: Article

References (22)

1. Bareither R, Pollard D (2011) A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need. Biotechnol Prog 27:2-14.
2. Betts JI, Baganz F (2006) Miniature bioreactors: current practices and future opportunities. Microb Cell Fact 5:21.
3. Chisti Y (1993) Animal cell culture in stirred bioreactors: observations on scale-up. Process Biochem 28:511-517.
4. Cohen SA, Michaud DP (1993) Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography.Anal Biochem 211:279-287.
5. Cook JA, Fox MH (1988) Effects of chronic pH 6.6 on growth, intracellular pH, and response to 42.0°C hyperthermia of Chinese hamster ovary cells. Cancer Res 48:2417-2420.
6. Gennaro LA, Salas-Solano O (2008) On-line CE-LIF-MS technology for the direct characterization of N-linked glycans from therapeutic antibodies. Anal Chem 80:3838-3845.
7. Guo D, Gao A, Michels DA, Feeney L, Eng M, Chan B, Laird MW, Zhang B, Yu XC, Joly J, Snedecor B, Shen A (2010) Mechanisms of unintended amino acid sequence changes in recombinant monoclonal antibodies expressed in Chinese Hamster Ovary (CHO) cells.BiotechnolBioeng 107:163-171.
8. Hopp J, Pritchett R, Darlucio M, Ma J, Chou JH (2009) Development of a high throughput protein a well-plate purification method for monoclonal antibodies. Biotechnol Prog 25:1427-1432.
9. Keane JT, Ryan D, Gray PP (2003) Effect of shear stress on expression of a recombinant protein by Chinese Hamster Ovary cells. Biotechnol Bioeng 81:211-220.
10. Kondragunta B, Drew JL, Brorson KA, Moreira AR, Rao G (2010) Advances in clone selection using high-throughput bioreactors. Biotechnol Prog 26:1095-1103.
11. Kumar S, Wittmann C, Heinzle E (2004) Minibioreactors. Biotechnol Lett 26:1-10.
12. LewisG, LuggR, LeeK, WalesR(2010)Novel automated microscale bioreactor technology: a qualitative and quantitative mimic for early process development. Bioprocess J 9:22-25.
13. Lin AA, Kimura R, Miller WM (1993) Production of tPA in recombinant CHO cells under oxygen-limited conditions. Biotechnol Bioeng 42:339-350.
14. Restelli V, Wang MD, Huzel N, Ethier M, Perreault H, Butler M (2006) The effect of dissolved oxygen on the production and the glycosylation profile of recombinant human erythropoietin produced fromCHOcells. BiotechnolBioeng 94:481-494.
15. Sauer PW, Burky JE, Wesson MC, Sternard HD, Qu L (2000) A high-yielding, generic fed-batch cell culture process for production of recombinant antibodies. Biotechnol Bioeng 67:585-597.
16. Tissot S, Oberbek A, Reclari M, Dreyer M, Hacker DL, Baldi L, Farhat M, Wurm FM (2011) Efficient and reproducible mammalian cell culture bioprocesses without probes and controllers? N Biotechnol 28:382-390.
17. Trummer E, Fauland K, Seidinger S, Schriebl K, Lattenmayer C, Kunert R, Vorauer-Uhl K, Weik R, Borth N, Katinger H, Muller D (2006) Process parameter shifting: part I. Effect of DOT, pH, and temperature on the performance of Epo- Fc expressing CHO cells cultivated in controlled batch bioreactors. Biotechnol Bioeng 94:1033-1044.
18. Tsao Y-S, Cardoso AG, Condon RGG, Voloch M, Lio P, Lagos JC, Kearns BG, Liu Z (2005) Monitoring Chinese hamster ovary cell culture by the analysis of glucose and lactate metabolism. J Biotechnol 118:316-327.
19. Varley J, Birch J (1999) Reactor design for large scale suspension animal cell culture. Cytotechnology 29:177-205.
20. Yoon SK, Choi SL, Song JY, Lee GM (2005) Effect of culture pH on Erythropoietin production by Chinese Hamster Ovary cells grown in suspension at 32.5 and 37.0°C. Biotechnol Bioeng 89:345-356.
21. Yuk IH, Baskar D, Duffy PH, Hsiung J, Leung S, Lin AA (2011) Overcoming challenges in WAVE bioreactors without feedback controls for pH and dissolved oxygen. Biotechnol Prog 27:1397-1406.
22. Zhang L, Lilyestrom W, Li C, Scherer T, van Reis R, Zhang B (2011) Revealing a positive charge patch on a recombinant monoclonal antibody by chemical labeling and mass spectrometry. Anal Chem 83:8501-8508.