Overcoming challenges in WAVE bioreactors without feedback controls for pH and dissolved oxygen

Biotechnology Progress

Volumn 27, Issue 5, 2011, Pages 1397-1406

  Yuk, Inn H ^a   Baskar, Dinesh ^a   Duffy, Philip H ^a   Hsiung, Jenny ^a   Leung, Susan ^a   Lin, Andy A ^a  

^a GENENTECH INC   (United States)

Author keywords

CHO cell culture; Dissolved oxygen; Gas transfer; PH; WAVE Bioreactor

Indexed keywords

BIOPHARMACEUTICAL INDUSTRY; CELL-FREE; CHO CELL; CHO CELL CULTURE; COST-EFFECTIVE METHODS; CULTURE CHAMBERS; CULTURING CELLS; DO CONTROL; GAS TRANSFER; HEADSPACES; KEY PARAMETERS; MONITORING AND CONTROL; THREE PARAMETERS;

ATMOSPHERIC COMPOSITION; BIOCONVERSION; BIOREACTORS; CARBON DIOXIDE; CELL CULTURE; CELL GROWTH; CELLS; DISSOLUTION; FEEDBACK CONTROL; FLOW OF GASES; GROWTH KINETICS; ROCKS;

DISSOLVED OXYGEN;

OXYGEN;

ANIMAL; ARTICLE; BIOREACTOR; CHEMISTRY; CHO CELL; CRICETULUS; HAMSTER; PH; SOLUBILITY;

ANIMALS; BIOREACTORS; CHO CELLS; CRICETINAE; CRICETULUS; HYDROGEN-ION CONCENTRATION; OXYGEN; SOLUBILITY;

EID: 80053900478     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.659     Document Type: Article

References (23)

1. Rao G, Moreira A, Brorson K. Disposable bioprocessing: the future has arrived. Biotechnol Bioeng. 2009; 102: 348-356.
2. Cronin CN, Lim KB, Rogers J. Production of selenomethionyl-derivatized proteins in baculovirus-infected insect cells. Prot Sci. 2007; 16: 2023-2029.
3. Haldankar R, Li D, Saremi Z, Baikalov C, Deshpande R. Serum-free suspension large-scale transient transfection of CHO cells in WAVE bioreactors. Mol Biotechnol. 2006; 34: 191-199.
4. Ling WL, Deng L, Lepore J, Cutler C, Cannon-Carlson S, Wang Y, Voloch M. Improvement of monoclonal antibody production in hybridoma cells by dimethyl sulfoxide. Biotechnol Prog. 2003; 19: 158-162.
5. Ye JX, Kober V, Tellers M, Naji Z, Salmon P, Markusen JF. High-level protein expression in scalable CHO transient transfection. Biotechnol Bioeng. 2009; 103: 542-551.
6. Singh V. Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnology 1999; 30: 149-158.
7. Mikola M, Seto J, Amanullah A. Evaluation of a novel wave bioreactor cellbag for aerobic yeast cultivation. Bioprocess Biosyst Eng. 2007; 30: 231-241.
8. Tang YJ, Ohashi R, Hamel JFP. Perfusion culture of hybridoma cells for hyperproduction of IgG(2a) monoclonal antibody in a wave bioreactor-perfusion culture system. Biotechnol Prog. 2007; 23: 255-264.
9. Hanson MA, Brorson KA, Moreira AR, Rao G. Comparisons of optically monitored small-scale stirred tank vessels to optically controlled disposable bag bioreactors. Microb Cell Fact. 2009; 8: 44.
10. Lin AA, Kimura R, Miller WM. Production of tPA in recombinant CHO cells under oxygen-limited conditions. Biotechnol Bioeng. 1993; 42: 339-350.
11. Link T, Backstrom M, Graham R, Essers R, Zorner K, Gatgens J, Burchell J, Taylor-Papadimitriou J, Hansson GC, Noll T. Bioprocess development for the production of a recombinant MUC1 fusion protein expressed by CHO-K1 cells in protein-free medium. J Biotechnol. 2004; 110: 51-62.
12. Miller WM, Blanch HW, Wilke CR. A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: effect of nutrient concentration, dilution rate, and pH. Biotechnol Bioeng. 1988; 32: 947-965.
13. Osman JJ, Birch J, Varley J. The response of GS-NS0 myeloma cells to pH shifts and pH perturbations. Biotechnol Bioeng. 2001; 75: 63-73.
14. Restelli V, Wang MD, Huzel N, Ethier M, Perreault H, Butler M. The effect of dissolved oxygen on the production and the glycosylation profile of recombinant human erythropoietin produced from CHO cells. Biotechnol Bioeng. 2006; 94: 481-494.
15. Yoon SK, Choi SL, Song JY, Lee GM. Effect of culture pH on erythropoietin production by Chinese hamster ovary cells grown in suspension at 32.5 and 37.0 degrees C. Biotechnol Bioeng. 2005; 89: 345-356.
16. Tissot S, Oberbek A, Reclari M, Dreyer M, Hacker DL, Baldi L, Farhat M, Wurm FM. Efficient and reproducible mammalian cell bioprocesses without probes and controllers? N Biotechnol 2011; 28: 382-390.
17. Trummer E, Fauland K, Seidinger S, Schriebl K, Lattenmayer C, Kunert R, Vorauer-Uhl K, Weik R, Borth N, Katinger H, Muller D. Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors. Biotechnol Bioeng. 2006; 94: 1033-1044.
18. Guo DL, Gao A, Michels DA, Feeney L, Eng M, Chan B, Laird MW, Zhang BY, Yu XC, Joly J, Snedecor B, Shen A. Mechanisms of unintended amino acid sequence changes in recombinant monoclonal antibodies expressed in Chinese Hamster Ovary (CHO) cells. Biotechnol Bioeng. 2010; 107: 163-171.
19. Johnson M, Lanthier S, Massie B, Lefebvre G, Kamen AA. Use of the Centritech lab centrifuge for perfusion culture of hybridoma cells in protein-free medium. Biotechnol Prog. 1996; 12: 855-864.
20. Bandyopadhyay B, Humphrey AE, Taguchi H. Dynamic measurement of the volumetric oxygen transfer coefficient in fermentation systems. Biotechnol Bioeng. 1967; 9: 533-544.
21. La estimation. Biotechnol Bioeng. 1990; 35: 1161-1163.
22. Royce PNC, Thornhill NF. Estimation of dissolved carbon dioxide concentrations in aerobic fermentations. AIChE J. 1991; 37: 1680-1686.
23. La. Biotechnol Bioeng. 1981; 23: 277-290.