Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells

Journal of Bioscience and Bioengineering

Volumn 114, Issue 6, 2012, Pages 577-581

  Kurosawa, Hiroshi ^a  

^a UNIVERSITY OF YAMANASHI   (Japan)

Author keywords

Adhesion; Dissociation; Embryonic stem cells; ROCK inhibitor; Y 27632

Indexed keywords

CELL SORTING; CRYO-PRESERVATION; EMBRYONIC STEM CELLS; MANIPULATION TECHNIQUES; PLURIPOTENT STEM CELLS; PROGRAMMED CELL DEATHS; PROTEIN KINASE; ROCK INHIBITORS; SINGLE CELLS; SPECIFIC INHIBITORS; STEM CELL RESEARCH; Y-27632;

ADHESION; CELL DEATH; DISSOCIATION; ENZYMES; GENE TRANSFER; PROTEINS; RESEARCH; ROCKS; STEM CELLS;

MOLECULAR BIOLOGY;

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE;

APOPTOSIS; CELL DIFFERENTIATION; CELL SELECTION; CRYOPRESERVATION; EMBRYONIC STEM CELL; HUMAN; PLURIPOTENT STEM CELL; QUALITY CONTROL; REVIEW; STEM CELL EXPANSION; STEM CELL RESEARCH;

AMIDES; APOPTOSIS; CELL DIFFERENTIATION; EMBRYONIC STEM CELLS; HUMANS; PLURIPOTENT STEM CELLS; PROTEIN KINASE INHIBITORS; PYRIDINES; RHO-ASSOCIATED KINASES; STEM CELL RESEARCH;

IPS;

EID: 84868159316     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2012.07.013     Document Type: Review

References (42)

1. Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones J.M. Embryonic stem cell lines derived from human blastocysts. Science 1998, 282:1145-1147.
2. Takahashi K., Tanabe K., Ohnuki M., Narita M., Ichikawa T., Tomoda K., Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007, 131:861-871.
3. Amit M., Carpenter M.K., Inokuma M.S., Chiu C., Harris C.P., Waknitz M.A., Itskovitz-Eldor J., Thomson J.A. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev. Biol. 2000, 227:271-278.
4. Watanabe K., Ueno M., Kamiya D., Nishiyama A., Matsumura M., Wataya T., Takahashi J.B., Nishikawa S., Nishikawa S., Muguruma K., Sasai Y. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 2007, 25:681-686.
5. Olson M.F. Applications for ROCK kinase inhibition. Curr. Opin. Cell Biol. 2008, 20:242-248.
6. Matsui T., Amano M., Yamamoto T., Chihara K., Nakafuku M., Ito M., Nakano T., Okawa K., Iwamatsu A., Kaibuchi K. Rho-associated kinase, a novel serine/threonine kinase, as a putative target for the small GTP binding protein Rho. EMBO J. 1996, 15:2208-2216.
7. Leung T., Manser E., Tan L., Lim L. A novel serine/threonine kinase binding the Ras-related Rho A GTPase which translocates the kinase to peripheral membranes. J. Biol. Chem. 1995, 270:29051-29054.
8. Ishizaki T., Maekawa M., Fujisawa K., Okawa K., Iwamatsu A., Fujita A., Watanabe N., Saito Y., Kakizuka A., Morii N., Narumiya S. The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. EMBO J. 1996, 15:1885-1893.
9. Riento K., Ridley A.J. Rocks: multifunctional kinases in cell behavior. Nat. Rev. Mol. Cell Biol. 2003, 4:446-456.
10. Uehata M., Ishizaki T., Satoh H., Ono T., Kawahara T., Marishita T., Tamakawa H., Yamagami K., Inui J., Maekawa M., Narumiya S. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature 1997, 389:990-994.
11. Ishizaki T., Uehata M., Tamechika I., Keel J., Nonomura K., Maekawa M., Narumiya S. Pharmacological properties of Y-27632, a specific inhibitor of Rho-associated kinase. Mol. Pharmacol. 2000, 57:976-983.
12. Asano T., Suzuki T., Tsuchiya M., Satoh S., Ikegaki I., Shibuya M., Suzuki Y., Hidaka H. Vasodilator actions of HA1077 in vitro and in vivo putatively mediated by the inhibition of protein kinase. Br. J. Pharmacol. 1989, 98:1091-1100.
13. Narumiya S., Ishizaki T., Uehata M. Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol. 2000, 325:273-284.
14. Koyanagi M., Takahashi J., Arakawa Y., Doi D., Fukuda H., Hayashi H., Narumiya S., Hashimoto N. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J. Neurosci. Res. 2008, 86:270-280.
15. Sato T., Vries R.G., Snippert H.J., Wetering M., Barker N., Stange D.E., Es J.H., Abo A., Kujala P., Peters P.J., Clevers H. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 2009, 459:262-265.
16. Chapman S., Liu X., Meyers C., Schlegel R., McBride A.A. Human keratinocytes are efficiently immortalized by a Rho kinase inhibitor. J. Clin. Invest. 2010, 120:2619-2626.
17. Emre N., Vidal J.G., Elia J., O'Connor E.D., Paramban R.I., Hefferan M.P., Navarro R., Goldberg D.S., Varki N.M., Marsala M., Carson C.T. The ROCK inhibitor y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. PLoS One 2010, 5:e12148.
18. Park I., Zhao R., West R.J., Yabuuchi A., Huo H., Ince T.A., Lerou P.H., Lensch M.W., Daley G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature 2008, 451:141-146.
19. Huangfu D., Osafune K., Maehr R., Guo W., Eijkelenboom A., Chen S., Muhlestein W., Melton D.A. Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat. Biotechnol. 2008, 26:1269-1275.
20. Warren L., Manos P.D., Ahfeldt T., Loh Y., Li H., Lau F., Ebina W., Mandal P.K., Smith Z.D., Meissner A. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 2010, 7:618-630. other 6 authors.
21. Fujioka T., Yasuchika K., Nakamura Y., Nakatsuji N., Suemori H. A simple and efficient cryopreservation method for primate embryonic stem cells. Int. J. Dev. Biol. 2004, 48:1149-1154.
22. Martin-Ibañez R., Unger C., Strömberg A., Baker D., Canals J.M., Hovatta O. Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor. Hum. Reprod. 2008, 23:2744-2754.
23. Li X., Meng G., Krawetz R., Liu S., Rancourt D.E. The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation. Stem Cell Dev. 2008, 17:1079-1085.
24. Li X., Krawetz R., Liu S., Meng G., Rancourt D.E. ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Hum. Reprod. 2009, 24:580-589.
25. Claassen D.A., Desler M.M., Rizzino A. ROCK inhibition enhances the recovery and growth of cryopreserved human embryonic stem cells and human induced pluripotent stem cells. Mol. Reprod. Dev. 2009, 76:722-732.
26. Mollamohammadi S., Taei A., Pakzad M., Totonchi M., Seifinejad A., Masoudi N., Baharvand H. A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells. Hum. Reprod. 2009, 24:2468-2476.
27. Baharvand H., Salekdeh G.H., Taei A., Mollamohammadi S. An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells. Nat. Protoc. 2010, 5:588-594.
28. Pakzad M., Totonchi M., Taei A., Seifinejad A., Hassani S.N., Baharvand H. Presence of a rock inhibitor in extracellular matrix supports more undifferentiated growth of feeder-free human embryonic and induced pluripotent stem cells upon passaging. Stem Cell Rev. Rep. 2010, 6:96-107.
29. Kitajima H., Niwa H. Clonal expansion of human pluripotent stem cells on gelatin-coated surface. Biochem. Biophys. Res. Commun. 2010, 396:933-938.
30. Peerani R., Rao B.M., Bauwens C., Yin T., Wood G.A., Nagy A., Kumacheva E., Zandstra P.W. Niche-mediated control of human embryonic stem cell self-renewal and differentiation. EMBO J. 2007, 26:4744-4755.
31. Sivasubramaniyan K., Pal R., Totey S., Bhat V.S., Totey S. Rho kinase inhibitor y27632 alters the balance between pluripotency and early differentiation events in human embryonic stem cells. Curr. Stem Cell Res. Ther. 2010, 5:2-12.
32. Osakada F., Jin Z., Hirami Y., Ikeda H., Danjyo T., Watanabe K., Sasai Y., Takahashi M. In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction. J. Cell Sci. 2009, 122:3169-3179.
33. Gauthaman K., Fong C.Y., Bongso A. Effect of ROCK inhibitor Y-27632 on normal and variant human embryonic stem cells (hESCs) in vitro: its benefits in hESC expansion. Stem Cell Rev. 2010, 6:86-95.
34. Sivasubramaiyan K., Totey S., Bhat V., Totey S.M., Deb K. Y-27632 enhances differentiation of blastocyst like cystic human embryoid bodies to endocrinologically active trophoblast cells on a biomimetic platform. J. Biomed. Sci. 2009, 16:88.
35. Chang T., Chen Y., Yang M., Chen C., Hsing E., Ko B., Liou J., Wu K.K. Rho kinases regulate the renewal and neural differentiation of embryonic stem cells in a cell plating density-dependent manner. PLoS One 2010, 5:e9187.
36. Frisch S.M., Screaton R.A. Anoikis mechanisms. Curr. Opin. Cell Biol. 2001, 13:555-562.
37. Nichols J., Smith A. Naive and primed pluripotent states. Cell Stem Cell 2009, 4:487-492.
38. Ohgushi M., Sasai Y. Lonely death dance of human pluripotent stem cells: ROCKing between metastable cell states. Trends Cell Biol. 2011, 21:274-282.
39. Krawetz R.J., Li X., Rancourt D.E. Human embryonic stem cells: caught between a ROCK inhibitor and a hard place. BioEssays 2009, 31:336-343.
40. Ohgushi M., Matsumura M., Eiraku M., Murakami K., Aramaki T., Nishiyama A., Muguruma K., Nakano T., Suga H., Ueno M. Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells. Cell Stem Cell 2010, 7:225-239. other 5 authors.
41. Chen G., Hou Z., Gulbranson D.R., Thomson J.A. Actin-myosin contractility is responsible for the reduced viability of dissociated human embryonic stem cells. Cell Stem Cell 2010, 7:240-248.
42. Yoshikawa H., Yoshioka K., Nakase T., Itoh K. Stimulation of ectopic bone formation in response to BMP-2 by Rho kinase inhibitor. Clin. Orthop. Relat. Res. 2009, 467:3087-3095.