Y-27632 enhances differentiation of blastocyst like cystic human embryoid bodies to endocrinologically active trophoblast cells on a biomimetic platform

Journal of Biomedical Science

Volumn 16, Issue 1, 2009, Pages

  Sivasubramaiyan, Kavitha ^a   Totey, Swapnil ^b   Bhat, Vijay ^c   Totey, Satish M ^b   Deb, Kaushik ^a  

^a Stempeutics Research Pvt Ltd   (India)

^b Stempeutics Research Malaysia Sdn Bhd   (Malaysia)

^c MANIPAL HOSPITAL   (India)

Author keywords

[No Author keywords available]

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; AGAROSE; CHORIONIC GONADOTROPIN; FIBRONECTIN; GELATIN; PROGESTERONE; SOMATOMEDIN B; ALPHA FETOPROTEIN; AMIDE; PROTEIN KINASE INHIBITOR; PYRIDINE DERIVATIVE; RHO KINASE; SEPHAROSE;

ARTICLE; BIOMIMETICS; BLASTOCYST; CELL DIFFERENTIATION; CELL LINEAGE; CONTROLLED STUDY; ENDODERM; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TROPHOBLAST; BIOLOGICAL MODEL; CELL LINE; CELL POLARITY; CULTURE MEDIUM; CYTOLOGY; CYTOPLASM; DRUG ANTAGONISM; DRUG EFFECT; EMBRYO DEVELOPMENT; EMBRYONIC STEM CELL; SECRETION;

ALPHA-FETOPROTEINS; AMIDES; BLASTOCYST; CELL DIFFERENTIATION; CELL LINE; CELL LINEAGE; CELL POLARITY; CHORIONIC GONADOTROPIN; CULTURE MEDIA; EMBRYONIC DEVELOPMENT; EMBRYONIC STEM CELLS; ENDODERM; FIBRONECTINS; GELATIN; HUMANS; MODELS, BIOLOGICAL; ORGANOIDS; PROGESTERONE; PROTEIN KINASE INHIBITORS; PYRIDINES; RHO-ASSOCIATED KINASES; SEPHAROSE; TROPHOBLASTS;

EID: 70349685316     PISSN: 10217770     EISSN: 14230127     Source Type: Journal    
DOI: 10.1186/1423-0127-16-88     Document Type: Article

References (14)

1. Methodologies to study implantation in mice. K Deb J Reese BC Paria, Methods Mol Med 2006 121 7 32
2. Endotoxin induced silencing of mesoderm induction and functional differentiation: Role of the DNA-binding cytokine HMGB1 in pluripotency and infection. K Sivasubramaniyan RR Atluri K Sarda M Arvind V Balaji K Deb, Regenerative Medicine 2008 3 1 23 31 10.2217/17460751.3.1.23 18154460
3. BMP4 initiates human embryonic stem cell differentiation to trophoblast. RH Xu X Chen DS Li R Li GC Addicks C Glennon TP Zwaka JA Thomson, Nat Biotech 2002 284 143 147
4. Thophoblast differentiation in embryoid bodies derived from human embryonic stem cells. B Gerami-Naini OV Dovzhenko N Durning FH Wegner JA Thompson TG Golos, Endocrinology 2004 145 1517 1524 10.1210/en.2003-1241 14684604
5. A role for Rho-like GTPases in the polarization of mouse eight-cell blastomeres. L Clayton A Hall MH Johnson, Dev Biol 1999 205 322 331 10.1006/dbio.1998.9117 9917367
6. Regulation of cadherin mediated cell-cell adhesion by the Rho family GTPases. K Kaibuchi S Kuroda M Fukata M Nakagawa, Curr Opin Cell Biol 1999 11 591 596 10.1016/S0955-0674(99)00014-9 10508646 (Pubitemid 29460400)
7. ROCK inhibitor permits survival of dissociated human embryonic stem cells. K Watanabe M Ueno D Kamiya A Nishiyama M Matsumura T Wataya JB Takahashi S Nishikawa S Nishikawa K Muguruma Y Sasai, Nat Biotechnol 2007 25 6 681 6 10.1038/nbt1310 17529971
8. Fibronectin and Cell Shape In Vivo: Studies on the Endometrium during Pregnancy. F Grinnell JR Head J Hoffpauir, J Cell Biol 1982 94 597 606 10.1083/jcb.94.3.597 7130273
9. FGF2 secreting human fibroblast feeder cells: a novel culture system for human embryonic stem cells. S Saxena M Hanwate K Deb V Sharma S Totey, Mol Reprod Dev 2008 75 10 1523 32 10.1002/mrd.20895 18318041
10. Blastocyst implantation precedes induction of insulin-like growth factor II gene expression in human trophoblasts. R Ohlsson E Larsson O Nilsson T Wahlström P Sundström, Development 1989 106 3 555 9 2598825
11. Testing of nine different xeno-free culture media for human embryonic stem cell cultures. K Rajala H Hakala S Panula S Aivio H Pihlajamäki R Suuronen O Hovatta H Skottman, Human Reproduction 2007 22 5 1231 1238 10.1093/humrep/del523 17251355
12. The Rho-Rock-Myosin signaling axis determines cell-cell integrity of self-renewing pluripotent stem cells. N Harb TK Archer N Sato, PLoS ONE 2008 3 8 3001 10.1371/journal.pone.0003001 18714354
13. Small guanosine triphosphate RhoA and Rho associated kinase as regulators of trophoblast migration. S Shiokawa M Iwashita Y Akimoto S Nagamatsu K Sakai H Hanashi M Kabir-Salmani Y Nakamura M Uehata Y Yoshimura, J Clin Endocrinol Metab 2002 87 12 5808 16 10.1210/jc.2002-020376 12466390
14. A role for Rho-like GTPases in the polarisation of mouse eight-cell blastomeres. L Clayton A Hall MH Johnson, Dev Biol 1999 205 2 322 31 10.1006/dbio.1998.9117 9917367