An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells

Nature Protocols

Volumn 5, Issue 3, 2010, Pages 588-594

  Baharvand, Hossein ^a,b   Salekdeh, Ghasem Hosseini ^a,c   Taei, Adeleh ^a   Mollamohammadi, Sepideh ^a  

^a ROYAN INSTITUTE FOR REPRODUCTIVE BIOMEDICINE   (Iran)

^b UNIVERSITY OF SCIENCE AND CULTURE   (Iran)

^c AGRICULTURAL BIOTECHNOLOGY RESEARCH INSTITUTE OF IRAN   (Iran)

Author keywords

[No Author keywords available]

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; MATRIGEL; RHO KINASE INHIBITOR;

ARTICLE; CELL CLONING; CELL CULTURE; CELL EXPANSION; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; EMBRYONIC STEM CELL; FREEZE THAWING; HUMAN; HUMAN CELL; INCUBATION TEMPERATURE; INCUBATION TIME; METHODOLOGY; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; THAWING;

AMIDES; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; COLLAGEN; CRYOPRESERVATION; CULTURE MEDIA; DRUG COMBINATIONS; EMBRYONIC STEM CELLS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; LAMININ; PROTEIN KINASE INHIBITORS; PROTEOGLYCANS; PYRIDINES; RHO-ASSOCIATED KINASES;

IPS;

EID: 77749324902     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.247     Document Type: Article

References (28)

1. Thomson, J.A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147 (1998).
2. Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872 (2007).
3. Yu, J. et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920 (2007).
4. Nishikawa, S., Goldstein, R.A. & Nierras, C.R. The promise of human induced pluripotent stem cells for research and therapy. Nat. Rev. Mol. Cell Biol. 9, 725-729 (2008).
5. Fujioka, T., Yasuchika, K., Nakamura, Y., Nakatsuji, N. & Suemori, H. A simple and efficient cryopreservation method for primate embryonic stem cells. Int. J. Dev. Biol. 48, 1149-1154 (2004).
6. Ji, L., de Pablo, J.J. & Palecek, S.P. Cryopreservation of adherent human embryonic stem cells. Biotechnol. Bioeng. 88, 299-312 (2004).
7. Heng, B.C., Kuleshova, L.L., Bested, S.M., Liu, H. & Cao, T. The cryopreservation of human embryonic stem cells. Biotechnol. Appl. Biochem. 41, 97-104 (2005).
8. Ware, C.B., Nelson, A.M. & Blau, C.A. Controlled-rate freezing of human ES cells. Biotechniques 38, 879-880, 882-883 (2005).
9. Reubinoff, B.E., Pera, M.F., Vajta, G. & Trounson, A.O. Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrifcation method. Hum. Reprod. 16, 2187-2194 (2001).
10. Richards, M., Fong, C.Y., Tan, S., Chan, W.K. & Bongso, A. An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells. Stem Cells 22, 779-789 (2004).
11. Zhou, C.Q., Mai, Q.Y., Li, T. & Zhuang, G.L. Cryopreservation of human embryonic stem cells by vitrification. Chin. Med. J. (Engl.) 117, 1050-1055 (2004).
12. Li, T., Zhou, C., Liu, C., Mai, Q. & Zhuang, G. Bulk vitrification of human embryonic stem cells. Hum. Reprod. 23, 358-364 (2008).
13. Watanabe, K. et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25, 681-686 (2007).
14. Heng, B.C. et al. Loss of viability during freeze-thaw of intact and adherent human embryonic stem cells with conventional slow-cooling protocols is predominantly due to apoptosis rather than cellular necrosis. J. Biomed. Sci. 13, 433-445 (2006).
15. Shi, J. & Wei, L. Rho kinase in the regulation of cell death and survival. Arch. Immunol. Ther. Exp. (Warsz.) 55, 61-75 (2007).
16. Koyanagi, M. et al. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J. Neurosci. Res. 86, 270-280 (2008).
17. Li, X., Krawetz, R., Liu, S., Meng, G. & Rancourt, D.E. ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Hum. Reprod. 24, 580-589 (2009).
18. Li, X., Meng, G., Krawetz, R., Liu, S. & Rancourt, D.E. The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation. Stem Cells Dev. 17, 1079-1085 (2008).
19. Lingor, P. et al. ROCK inhibition and CNTF interact on intrinsic signaling pathways and differentially regulate survival and regeneration in retinal ganglion cells. Brain 131, 250-263 (2008).
20. Martin-Ibañez, R. et al. Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor. Hum. Reprod. 23, 2744-2754 (2008).
21. Claassen, D.A., Desler, M.M. & Rizzino, A. ROCK inhibition enhances the recovery and growth of cryopreserved human embryonic stem cells and human induced pluripotent stem cells. Mol. Reprod. Dev. 76, 722-732 (2009).
22. Mollamohammadi, S. et al. A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells. Hum. Reprod. 24, 2468-2476 (2009).
23. Krawetz, R.J., Li, X. & Rancourt, D.E. Human embryonic stem cells: caught between a ROCK inhibitor and a hard place. Bioessays 31, 336-343 (2009).
24. Klimanskaya, I., Chung, Y., Becker, S., Lu, S.J. & Lanza, R. Derivation of human embryonic stem cells from single blastomeres. Nat. Protoc. 2, 1963-1972 (2007).
25. Suemori, H. & Nakatsuji, N. Growth and differentiation of cynomolgus monkey ES cells. Methods Enzymol. 365, 419-429 (2003).
26. Pakzad, M. et al. Presence of a ROCK inhibitor in extracellular matrix supports more undifferentiated growth of feeder-free human embryonic and induced pluripotent stem cells upon passaging. Stem Cell Rev. published online, doi:10.1007/s12015-009-9103-z (12 December 2009).
27. Bajpai, R., Lesperance, J., Kim, M. & Terskikh, A.V. Efficient propagation of single cells Accutase-dissociated human embryonic stem cells. Mol. Reprod. Dev. 75, 818-827 (2008).
28. Masters, J.R. & Stacey, G.N. Changing medium and passaging cell lines. Nat. Protoc. 2, 2276-2284 (2007).